전북지역 소 설사유래 병원성대장균 감염실태 조사
Prevalence of enterovirulent Escherichia coli from diarrhea of cattles in Jeonbuk, Korea
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Enterovirulent Escherichia coli are among the most important causes of diarrhea in cattles. Between January and December, 2017, a total of 150 stool specimens from cattles were investigated for entero-haemorrhagic E. coli (EHEC), enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), en-terotoxigenic E. coli (ETEC) and enteroinvasive E. coli (EIEC) using real-time PCR. 131 E. coli were isolated from feces. The most frequently isolated pathotype in feces was EHEC (37 isolates). EPEC, ETEC and EAEC were detected in feces with 14, 7 and 3 respectively. EIEC was not detected. Antimicrobial resistance test was performed by agar disc diffusion method with 14 antimicrobials. Enterovirulent E. coli isolates showed the highest antimicrobial resistance to ampicillin 61.3%, followed by tetracycline 54.5% and streptomycin 45.5%. They had low resistance to amikacin 11.4%. Of 44 isolates, 37 (84.1%) were resistant to more than 2 antimicrobials. futher study a highest antimicrobial susceptibility to trime-thoprim/sulfamethoxazole 50.0% and florofenicol 47.7%.
Abstract
Enterovirulent Escherichia coli are among the most important causes of diarrhea in cattles. Between January and December, 2017, a total of 150 stool specimens from cattles were investigated for entero-haemorrhagic E. coli (EHEC), enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), en-terotoxigenic E. coli (ETEC) and enteroinvasive E. coli (EIEC) using real-time PCR. 131 E. coli were isolated from feces. The most frequently isolated pathotype in feces was EHEC (37 isolates). EPEC, ETEC and EAEC were detected in feces with 14, 7 and 3 respectively. EIEC was not detected. Antimicrobial resistance test was performed by agar disc diffusion method with 14 antimicrobials. Enterovirulent E. coli isolates showed the highest antimicrobial resistance to ampicillin 61.3%, followed by tetracycline 54.5% and streptomycin 45.5%. They had low resistance to amikacin 11.4%. Of 44 isolates, 37 (84.1%) were resistant to more than 2 antimicrobials. futher study a highest antimicrobial susceptibility to trime-thoprim/sulfamethoxazole 50.0% and florofenicol 47.7%.
참고문헌 (19)
- 김봉환, "자돈의 하리성 질병의 예방과 치료(상)", 대한수의사회지, 17, pp.16~22 (1981)
- 도 국가항생제 사용 및 내성 모니터링-가축, 축산물-, "임숙경", 농림축산검역본부, 식품의약품안전평가원. 마마디자인, ~ (2015)
- 채희선, "소의 분변과 도체에서 shiga toxin-producing Escherichia coli의 분리와 특성", 한국동물위생학회지, 32(3), pp.241~249 (2009), 한국동물위생학회
- 김미나, "다중 PCR법으로 진단한 Verotoxin 생성 Escherichia coli O157:H7 출혈성 장염인 영아 1예", Annals of Clinical Microbiology, 13(2), pp.85~89 (2010), 대한임상미생물학회
- Cho JK, "Antimicrobial drug resistance of Escherichia coli isolated from cattle, swine and chicken", Kor J Vet Publ Hlth, 30, pp.9~18 (2006)
- 최창용, "신속 진단 킷트를 활용한 한우 송아지의 설사증 원인체 검사", 한국동물위생학회지, 40(1), pp.61~66 (2017), 한국동물위생학회
- Clarke SC, "Virulence of enteropathogenic Escherichia coli, a global pathogen", Clin Microbiol Rev, 16, pp.365~378 (2003)
- Furuhata K, "Prevalence and serotypes of verocytotoxinproducing Escherichia coli(VTEC)isolates from diary cattle", Kansenshogaku Zasshi, 73, pp.445~450 (1999)
- Honda T, "Factors influencing the development of hemolytic uremic syndrome caused by enterohemorrhagic Escherichia coli infection : from a questionaire survey to in vitro experiment", Pediatr Int, 41, pp.209~212 (1999)
- Kaper JB, "Escherichia coli O157: H7 and other Shiga toxin-producing E. coli strains", pp.163~182 (1998), American Society for Microbiology
- Kaper JB, "Defining EPEC. In : Proceedings of the International Symposium on Enteropathogenic Escherichia coli(EPEC)", Rev Microbiol, 27, pp.130~133 (1996)
- 김홍태, "유통되는 쇠고기에서 분리한 대장균의 항생제 내성 조사.연구 (2)", 한국동물위생학회지, 32(1), pp.93~102 (2009), 한국동물위생학회
- Kim HY, "Domestic outbreaks of calves’ diarrhea in Korea", Korean J Buiat, 21, pp.61~81 (2016)
- 이봉주, "송아지 설사분변으로부터 Shiga toxin-producingEscherichia coli 의 분리 및 특성규명", 대한수의학회지, 46(2), pp.135~142 (2006), 대한수의학회
- Nataro JP, "Diarrheagenic Escherichia coli", Clin Microbiol Rev, 11, pp.142~201 (1998)
- Okhuysen PC, "Enteroaggregative Escherichia coli(EAEC) : a cause of acute and persistent diarrhea of worldwide importance", J Infect Dis, 202, pp.503~505 (2010)
- So M, "Characterization of an Escherichia coli plasmid encoding for synthesis of heat-labile toxin : molecular cloning of the toxin determinant", Infect Immun, 21, pp.405~411 (1978)
- 전무형, "도축돈 장분변으로부터 Shiga Toxin-Producing Escherichia coli의 분리와 성상", 대한수의학회지, 44(4), pp.551~559 (2004), 대한수의학회
- Tarr PI, "Escherichia coli O157 : H7 and the hemolytic uremic syndrome : importance of early cultures in establishing the etiology", J Infect Dis, 162, pp.553~556 (1990)