Viable Bacterial Cell Patterning Using a Pulsed Jet Electrospray System
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
In the present study, drop-on-demand two-dimensional patterning of unstained and stained bacterial cells on untreated clean wafers was newly conducted using an electrospray pulsed jet. We produced various spotted patterns of the cells on a silicon wafer by varying the experimental conditions, such as the frequency, flow rate, and translational speed of the electrospray system in a two-dimensional manner. Specifically, the electrospray’s pulsed jet of cell solutions produced alphabetical patterns consisting of spots with a diameter of approximately 10 μm, each of which contained a single or a small number of viable bacteria. We tested the viability of the patterned cells using two visualization methods. This pattering technique is newly tested here and it has the potential to be applied in a variety of cell biology experiments.
Abstract
In the present study, drop-on-demand two-dimensional patterning of unstained and stained bacterial cells on untreated clean wafers was newly conducted using an electrospray pulsed jet. We produced various spotted patterns of the cells on a silicon wafer by varying the experimental conditions, such as the frequency, flow rate, and translational speed of the electrospray system in a two-dimensional manner. Specifically, the electrospray’s pulsed jet of cell solutions produced alphabetical patterns consisting of spots with a diameter of approximately 10 μm, each of which contained a single or a small number of viable bacteria. We tested the viability of the patterned cells using two visualization methods. This pattering technique is newly tested here and it has the potential to be applied in a variety of cell biology experiments.
참고문헌 (10)
- Gittens JE, "Biotic sol-gel coating for the inhibition of corrosion in seawater", ECS Trans, 24, pp.211~229 (2010)
- Hayati I, "Mechanism of stable jet formation in electrohydrodynamic atomization", Nature, 319, pp.41~43 (1986)
- Kim K, "Use of an electrospray for the generation of bacterial bioaerosols", J. Aerosol Sci, 39, pp.365~372 (2008)
- Kim K, "Drop-ondemand patterning of bacterial cells using pulsed jet electrospraying", Anal. Chem, 82, pp.2109~2112 (2010)
- Liu VA, "Engineering protein and cell adhesivity using PEO-terminated triblock polymers", J. Biomed. Mater. Res. A, 60, pp.126~134 (2002)
- Mrksich M, "Using microcontact printing to pattern the attachment of mammalian cells to self-assembled monolayers of alkanethiolates on transparent films of gold and silver", Exp. Cell Res, 235, pp.305~313 (1997)
- Park J, "High-resolution electrohydrodynamic jet printing", Nat. Mater, 6, pp.782~789 (2007)
- 이상엽, "Microcontact Printing of Biotin for Selective Immobilization of Streptavidin-fused Proteins and SPR Analysis", Biotechnology and Bioprocess Engineering, 9(2), pp.137~142 (2004), 한국생물공학회
- Park TJ, "Protein nanopatterns and biosensors using gold binding polypeptide as a fusion partner", Anal. Chem, 78, pp.7197~7205 (2006)
- Xu L, "Microcontact printing of living bacteria arrays with cellular resolution", Nano Lett, 7, pp.2068~2072 (2007)