Fabrication of Poly(diallyldimethylammonium chloride) - Patterned Substrates for Patterning of Single Strand DNA Using Ion Implantation
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In this study, a convenient method for the selective immobilization of single strand DNA(ssDNA) on a polymer surface was described. A positively charged polyelectrolyte, poly(diallyldimethylammoniumchloride) (PDDA), was spin-coated on a tissue culture petridish and the micropatternsof the PDDA were formed by selective ion implantation through a pattern mask. The surfaceproperty of the implanted PDDA was investigated by using a surface profiler and FT-IRspectrometer. Cy3-labeled ssDNA was selectively immobilized on the PDDA patterns throughionic interaction and thus, well-defined ssDNA patterns were obtained.
Abstract
In this study, a convenient method for the selective immobilization of single strand DNA(ssDNA) on a polymer surface was described. A positively charged polyelectrolyte, poly(diallyldimethylammoniumchloride) (PDDA), was spin-coated on a tissue culture petridish and the micropatternsof the PDDA were formed by selective ion implantation through a pattern mask. The surfaceproperty of the implanted PDDA was investigated by using a surface profiler and FT-IRspectrometer. Cy3-labeled ssDNA was selectively immobilized on the PDDA patterns throughionic interaction and thus, well-defined ssDNA patterns were obtained.
참고문헌 (14)
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