소음자극 후 마우스 내이에서 TNF-Alpha의 발현과 분포 양상
Expression and Distribution of Tumor Necrosis Factor-Alpha in Mice Cochlea Exposed to Noise
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Background and Objectives Recent studies have shown that inflammatory responses occur in the inner ear under various damaging conditions including noise-overstimulation. Identification of time-dependent expression patterns of pro-inflammatory cytokines during the response initiation should lead to rational therapeutic strategies that block the response and reduce the damaging sequelae. Materials and Method We evaluated the time-dependent expression pattern of pro-inflammatory cytokines in noise-exposed mouse cochlea (white noise, 120 dB SPL, 3 hours) using immunohistochemistry and reverse transcriptase-polymerase chain reaction (RT-PCR). Results The most potent pro-inflammatory cytokine, the tumor necrosis factor-alpha (TNF-α), was up-regulated after noise exposure. Immunohistochemical analyses showed that the TNF-α expression was distinctively induced within the spiral ganglion and stria vascularis. RT-PCR showed that TNF-α was induced shortly after noise exposure and persisted upto seven days following noise exposure. Conclusion Taken together, acoustic trauma induces cochlear inflammation and the data suggest that TNF-α may have some role in cochlea damage that occur following noise exposure. Korean J Otorhinolaryngol-Head Neck Surg 2010;53:527-33
Abstract
Background and Objectives Recent studies have shown that inflammatory responses occur in the inner ear under various damaging conditions including noise-overstimulation. Identification of time-dependent expression patterns of pro-inflammatory cytokines during the response initiation should lead to rational therapeutic strategies that block the response and reduce the damaging sequelae. Materials and Method We evaluated the time-dependent expression pattern of pro-inflammatory cytokines in noise-exposed mouse cochlea (white noise, 120 dB SPL, 3 hours) using immunohistochemistry and reverse transcriptase-polymerase chain reaction (RT-PCR). Results The most potent pro-inflammatory cytokine, the tumor necrosis factor-alpha (TNF-α), was up-regulated after noise exposure. Immunohistochemical analyses showed that the TNF-α expression was distinctively induced within the spiral ganglion and stria vascularis. RT-PCR showed that TNF-α was induced shortly after noise exposure and persisted upto seven days following noise exposure. Conclusion Taken together, acoustic trauma induces cochlear inflammation and the data suggest that TNF-α may have some role in cochlea damage that occur following noise exposure. Korean J Otorhinolaryngol-Head Neck Surg 2010;53:527-33
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