Lipopolysaccharide을 이용한 생쥐 중이염모델에서의 Interleukin-10의 역할
Role of Interleukin-10 on Mouse Otitis Media Model Using Lipopolysaccharide
2
조회
0
다운로드
4
인용
0
참고문헌
초록
Background and Objectives Interleukin (IL)-10 is a major regulator of innate immunity. It interferes with the production of inflammatory mediators by reacting with polymorphonuclear neutrophils, monocytes and macrophages. In addition, it also up-regulates the ex-pression of molecules that amplify the anti-inflammatory effect of IL-10. This study tested the hypothesis that injection of various concentrations of exogenous IL-10 in the middle ear cleft can inhibit the acute stage of otitis media induced by lipopolysaccharide (LPS). And if so, the level of concentration for inhibition can be determined. Materials and Method IL-10 and/or LPS were injected transtympanically into the middle ear cavity of normal mice. Histopathological measurement of submucosal thickness and num-ber of inflammatory cell infiltration was performed at 24 hours after inoculation of IL-10 and/or LPS. Results At 24 hours after inoculation, every group of IL-10 inoculation reduced both thickening of the mucous and submucosal infiltration of inflammatory cells in a dose-dependent manner. As the concentrations of IL-10 inoculated increased, mucosal thickness and submucosal infiltration of inflammatory cells were more reduced. Conclusion This study demonstrated that exogenous IL-10 injection into the middle ear can directly reduce LPS-induced otitis media in acute stage in a dose-dependent manner. These findings also indicate that IL-10 may be a candidate for local modulator of acute otitis media.
Abstract
Background and Objectives Interleukin (IL)-10 is a major regulator of innate immunity. It interferes with the production of inflammatory mediators by reacting with polymorphonuclear neutrophils, monocytes and macrophages. In addition, it also up-regulates the ex-pression of molecules that amplify the anti-inflammatory effect of IL-10. This study tested the hypothesis that injection of various concentrations of exogenous IL-10 in the middle ear cleft can inhibit the acute stage of otitis media induced by lipopolysaccharide (LPS). And if so, the level of concentration for inhibition can be determined. Materials and Method IL-10 and/or LPS were injected transtympanically into the middle ear cavity of normal mice. Histopathological measurement of submucosal thickness and num-ber of inflammatory cell infiltration was performed at 24 hours after inoculation of IL-10 and/or LPS. Results At 24 hours after inoculation, every group of IL-10 inoculation reduced both thickening of the mucous and submucosal infiltration of inflammatory cells in a dose-dependent manner. As the concentrations of IL-10 inoculated increased, mucosal thickness and submucosal infiltration of inflammatory cells were more reduced. Conclusion This study demonstrated that exogenous IL-10 injection into the middle ear can directly reduce LPS-induced otitis media in acute stage in a dose-dependent manner. These findings also indicate that IL-10 may be a candidate for local modulator of acute otitis media.
참고문헌 (19)
- Gates, "Acute otitis media and otitis media with effusion. In Otolaryngology Head and Neck Surgery. 3rd ed", pp.461~477 (1998), Mosby Year Book
- Yellon RF, "Characterization of cytokines present in middle ear effusions", Laryngoscope, 101(2), pp.165~169 (1991)
- 정민교, "중이염의 발병기전에 있어 염증매개체들의 역할", 대한이비인후과학회지 두경부외과학, 50(1), pp.2~13 (2007), 대한이비인후과학회
- Smirnova MG, "The immunoregulatory and allergy-associated cytokines in the aetiology of the otitis media with effusion", Mediators Inflamm, 13(2), pp.75~88 (2004)
- Barzilai A, "Dynamics of interleukin-1 production in middle ear fluid during acute otitis media treated with antibiotics", Infection, 27(3), pp.173~176 (1999)
- Fergie JE, "The role of inflammatory mediators and antiinflammatory drugs in otitis media", Pediatr Ann, 27(2), pp.76~81 (1998)
- Dinarello CA, "An overview on interleukin-1 as a therapeutic agent", Biotherapy, 1(4), pp.245~254 (1989)
- Ovesen T, "Bacteria and endotoxin in middle ear fluid and the course of secretory otitis media", Clin Otolaryngol Allied Sci, 17(6), pp.531~534 (1992)
- Willett DN, "Relationship of endotoxin to tumor necrosis factor-alpha and interleukin-1 beta in children with otitis media with effusion", Ann Otol Rhinol Laryngol, 107(1), pp.28~33 (1998)
- Moore KW, "Interleukin-10 and the interleukin-10 receptor", Annu Rev Immunol, 19, pp.683~765 (2001)
- Kuhn R, "Interleukin-10-deficient mice develop chronic enterocolitis", Cell, 75(2), pp.263~274 (1993)
- Ozturk K, "Dose-related effects of recombinant human interleukin-10 on hypoxia-induced skeletal muscle injury in immature rats", J Orthop Sci, 11(6), pp.620~625 (2006)
- Qian L, "Interleukin-10 protects lipopolysaccharide-induced neurotoxicity in primary midbrain cultures by inhibiting the function of NADPH oxidase", J Pharmacol Exp Ther, 319(1), pp.44~52 (2006)
- Zhang X, "Suppression of early experimental osteoarthritis by gene transfer of interleukin-1 receptor antagonist and interleukin-10", J Orthop Res, 22(4), pp.742~750 (2004)
- Hebda PA, "Upregulation of messenger RNA for inflammatory cytokines in middle ear mucosa in a rat model of acute otitis media", Ann Otol Rhinol Laryngol, 107(6), pp.501~507 (1998)
- Hebda PA, "Cytokine profiles in a rat model of otitis media with effusion caused by eustachian tube obstruction with and without Streptococcus pneumoniae infection", Laryngoscope, 112(9), pp.1657~1662 (2002)
- Melhus A, "Effects of amoxicillin on the expression of cytokines during experimental acute otitis media caused by non-typeable Haemophilus influenzae", J Antimicrob Chemother, 48(3), pp.397~402 (2001)
- Melhus A, "Expression of cytokine genes during pneumococcal and nontypeable Haemophilus influenzae acute otitis media in the rat", Infect Immun, 68(7), pp.4024~4031 (2000)
- Skotnicka B, "Cytokines in children with otitis media with effusion", Eur Arch Otorhinolaryngol, 257(6), pp.323~326 (2000)