흰쥐에서 소음자극 후 Hypoxia-Inducible Factor의 내이내의 발현
Expression of Hypoxia-Inducible Factor in the Inner Ear of Noise-Exposed Mouse
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Abstract
：Investigations regarding the mechanisms of noise-induced hearing los have sugested that noise exposure induces tissue ischemia, and damages the inner ear. The hypoxia-inducible factor (HIF), a helix-lop-helix PAS transcription factor, is known to prevent hypoxic cel damage in the brain in cases of ischemic attacks. Like the role it plays in the brain, there is a posibility that HIF could be found in the inner ear playing the similar role. Materials and Method：We used 23 (46 ears)threshold was measured by ABR before and after exposing mice to 120 dB SPL broad band white noise 3 hours daily for 5 consecutive days. And after 1 month, the hearing threshold was rechecked to assure the permanent threshold shift. Cochlea were collected for the histological study and the expression of HIF was observed. Results：Control mice showed hearing level of 27.5± 1.7 dB (mean± SD), which was changed to 80.5± 2.1 dB immediately after 5-day of exposure to noise, and 79.5±s noticeable. HIF was expresed in the spiral limbus, few suporting cels, stria vascularis and spiral ganglion cells of the noise exposed mouse. Conclusion：Noise, a form of acoustic trauma to the inner ear, induced damage to the outer hair cels in mice. At the same time, HIF were expresed in all of the studied animals. The posibility of HIF playing a role of protecting the inner ear from the acoustic trauma as it does in the brain should be investigated further.
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