소음 노출후 와우측벽에서 KCNJ10 K+채널의 발현양상
Expression Patterns of KCNJ10 K+ Channel in the Cochlear Lateral Wall After Acoustic Trauma
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
It is well known that noise exposure leads to the sensory hair cell loss and other neuronal damage in the cochlea. But recently it has been reported that noise exposure could also damage lateral wall of cochlea such as stria vascularis and spiral ligament. K+ is the major cation in endolymph and important to maintain homeostasis within the cochlea. We have investigated the expression patterns of KCNJ10 K+ channel in noise induced cochlear damage. Materials and Method： Twenty adult male guinea pigs (300-350 g) were included in this study. In experimental group (n=16), acoustic trauma was induced by continuous broad band noise for 2 hr to 115 dB SPL and broad band noise for 6 hr to 120 dB SPL with 3 consecutive days. After noise exposure, auditory brainstem response threshold shift and hair cell loss were evaluated. A study for KCNJ10 K+ channel expression was examined by immunohistochemical staining. Results：After noise exposure, auditory brainstem response showed transient threshold shift (TTS) and permanent threshold shift (PTS) in accordance with noise exposure. The expression patterns of CKNJ10 K+ channel were changeable in TTS group. But there were no change of expression patterns in PTS group. Conclusion：In the cochlear lateral wall, KCNJ10 K+ channel expressions were affected with noise exposure and these changes might be associated with the regulation of homeostasis in the cochlea lateral wall. (Korean J Otorhinolaryngol- Head Neck Surg 2008;51:312-8)
Abstract
It is well known that noise exposure leads to the sensory hair cell loss and other neuronal damage in the cochlea. But recently it has been reported that noise exposure could also damage lateral wall of cochlea such as stria vascularis and spiral ligament. K+ is the major cation in endolymph and important to maintain homeostasis within the cochlea. We have investigated the expression patterns of KCNJ10 K+ channel in noise induced cochlear damage. Materials and Method： Twenty adult male guinea pigs (300-350 g) were included in this study. In experimental group (n=16), acoustic trauma was induced by continuous broad band noise for 2 hr to 115 dB SPL and broad band noise for 6 hr to 120 dB SPL with 3 consecutive days. After noise exposure, auditory brainstem response threshold shift and hair cell loss were evaluated. A study for KCNJ10 K+ channel expression was examined by immunohistochemical staining. Results：After noise exposure, auditory brainstem response showed transient threshold shift (TTS) and permanent threshold shift (PTS) in accordance with noise exposure. The expression patterns of CKNJ10 K+ channel were changeable in TTS group. But there were no change of expression patterns in PTS group. Conclusion：In the cochlear lateral wall, KCNJ10 K+ channel expressions were affected with noise exposure and these changes might be associated with the regulation of homeostasis in the cochlea lateral wall. (Korean J Otorhinolaryngol- Head Neck Surg 2008;51:312-8)
참고문헌 (15)
- Hirose K, "Lateral wall histopathology and endocochlear potential in the noise-damaged mouse cochlea", J Assoc Res Otolaryngol, 4(3), pp.339~352 (2003)
- Wangemann P, "K+ cycling and the endocochlear potential", Hear Res, 165(1), pp.1~9 (2002)
- Hibino H, "Expression of an inwardly rectifying K+ channel, Kir5.1, in specific types of fibrocytes in the cochlear lateral wall suggests its functional importance in the establishment of endocochlear potential", Eur J Neurosci, 19(1), pp.76~84 (2004)
- Salt AN, "Accumulation of potassium in scala vestibule perilymph of the mammalian cochlea", Ann Otol Rhinol Laryngol, 102(1), pp.64~70 (1993)
- Konishi T, "Ion transport in guinea pig cochlea. I. Potassium and sodium transport", Acta Otolaryngol, 86(1), pp.22~34 (1978)
- Jin Z, "Developmental expression and localization of KCNJ10 K+ channels in the guinea pig inner ear", Neuroreport, 17(5), pp.475~479 (2006)
- Raphael Y, "Prenatal maturation of endocochlear potential and electrolyte composition of inner ear fluids in guinea pigs", Arch Otorhinolaryngol, 237(2), pp.147~152 (1983)
- Crouch JJ, "Immunohistochemical localization of the Na-K-Cl cotrnaspoter(NKCC1) in the gerbil inner ear", J Histochem Cytochem, 45(6), pp.773~778 (1997)
- Delpire E, "Deafness and imbalance associated with inactivation of the secretory Na-K-2Cl cotransforter", Nat Genet, 22(2), pp.192~195 (1999)
- Schulte BA, "Distribution of immunoreactive Na+, K+-ATPase in gerbil cochlea", J Histochem Cytochem, 37(2), pp.127~134 (1989)
- Boettcher FA, "Distortion-product otoacoustic emissions in Mongolian gervils with resistance to noise-induced hearing loss", J Acoust Soc Am, 98(6), pp.3215~3222 (1995)
- Benitez LD, "Temporary threshold shifts in chinchilla: Electophysiological correlates", J Acoust Soc Am, 52(4), pp.1115~1123 (1972)
- Ma YL, "Combined effects of adrenalectomy and noise exposure on compound action potentials, endocochlear potentials and endolympnatic potassium concentrations", Hear Res, 91(1), pp.79~86 (1995)
- Ide M, "Long term effects of intense sound on endocochlear DC potential", Auris Nasus Larynx, 17(1), pp.1~10 (1990)
- Wang JA, "Changes in endocochlear potential during anoxia after intense noise exposure", Hear Res, 44(2), pp.143~149 (1990)