Alocasia cadieri Chantrier의 기내번식
In Vitro Propagation of Alocasia cadieri Chantrier
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초록
본 실험은 Alocasia cadieri Chantrier를 기내배양하여 일시에 균일한 식물체를 대량생산하기 위하여 실시하였다. Alocasia 의 경정을 TDZ 0.1 mg/L가 첨가된 배지에 배양하였을 때 신초증식이 가장 높았으며, 형성된 신초에서 신초의 증식은 TDZ과 NAA가 각각 0.5 mg/L 첨가된 배지에서 효과적이었 다. 형성된 신초의 발근은 IBA 또는 NAA가 첨가된 배지보다 는 활성탄 2.0 g/L가 첨가된 배지에서 양호하였으며, 소식물체 의 순화는 perlite : vermiculite가 1:1로 혼합된 용토 또는 vermiculite가 적합하였다.
Abstract
In order to micropropagate uniform plantlets of Alocasia cadieri Chantrier in vitro, the shoot tips werecultured on media containing various concentrations of BA and thidiazuron (TDZ). Multiple shoot formation fromshoot tips was very effective on medium containing 0.1 mg/L TDZ. The formed shoots from shoot tips wereseparated into a shoot, and cultured on media with BA, TDZ, and NAA combination for proliferation. The shootswere multiplied very vigorously on medium with 0.5 mg/L TDZ and 0.5 mg/L NAA. The rooting and growth ofmultiplied shoots were more effective on medium with 2.0 g/L activated charcoal, rather than those with IBA andNAA. Rooted plantlets show high survival in soil mixed with perlite 1 : vermiculite 1 or vermiculite alone.
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