다아체 형성에 의한 Philodendron wend-imbe의 대량번식
Microropagation of Philodendron wend-imbe through Adventitious Multi-bud Cluster Formation
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참고문헌
초록
본 실험은 Philodendron wend-imbe를 기내배양하여 일시에 균일한 식물체를 대량생산하기 위하여 실시하였다. Philodendron의 경정에서 다아체 형성은 BA 5.0～10.0 mg/L 또는 TDZ 0.05～0.1 mg/L가 첨가된 MS 배지에서 양호하였다. 형성된 다아체 절편체 (5～7 mm)를 BA 5.0 mg/L와 sucrose 20 g/L가 첨가된 MS 배지에서 배양한 결과, 신초 및 다아체의 증식이 매우 양호하였으며, 신초기부에서 callus 발생이 억제되었다. 다아체 절편체에서 신초의 발생 및 발근은 IBA 1.0～2.0 mg/L 또는 NAA 0.1 mg/L가 첨가된 배지가 효과적이었으며, 발근된 신초의 순화는 perlite와 peat moss의 1:1 혼합용토 또는 peat moss가 적합하였다.
Abstract
In order to micropropagate uniform plantlets of Philodendron wend-imbe, the shoot tips were cultured on media supplemented with 0.5∼10.0 mg/L BA or 0.01∼1.0 mg/L thidiazuron (TDZ). The multi-bud clusters from basal part of shoots formed vigorously on media containing 5.0∼10.0 mg/L BA or 0.05∼0.1 mg/L TDZ. Shoot formation from the bud cluster sections (5∼7 mm) was achieved favorably on medium with 5.0 mg/L BA and 20 g/L sucrose. Lowering of sucrose in medium to 20 g/L was effective for the inhibition of callus growth from basal part of shoots. Growth of shoots and their rooting were favorable on media containing 1.0∼2.0 mg/L IBA or 0.1 mg/L NAA. The rooted plantlets were acclimatizated effectively in soil mixed with perlite 1 : peat moss 1 or peat moss alone.
참고문헌 (14)
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