LPS로 유도된 Raw 264.7 세포에서 銀翹散 열수 추출물의 항염증 효과

Abstract

Objectives : This research sought to examine the anti-inflammatory properties of Eungyo-san (EGS) water extract in vitro using RAW 264.7 macrophage cells. Methods : RAW 264.7 cells were pretreated with various concentrations of EGS (30–1000 μg/mL) for 1 hour and then stimulated with lipopolysaccharide (LPS, 1 μg/mL). Cell viability was measured via MTT assay. The synthesis of pro-inflammatory cytokines(TNF-α, IL-6, IL-1β) and nitric oxide(NO) was assessed. Protein expression levels of iNOS, NF-κB, IκBα, and MAPKs were analyzed by immunoblotting. Results : EGS exhibited no cytotoxicity up to 1000 μg/mL. Pretreatment with EGS significantly inhibited LPS-induced NO production and reduced the expression of iNOS. Moreover, EGS decreased the levels of pro-inflammatory cytokines and PGE2. EGS also suppressed LPS-induced degradation of IκBα and nuclear translocation of NF-κB. Among MAPKs, only JNK phosphorylation was attenuated at the highest EGS concentration. Conclusions : These findings suggest that EGS exerts anti-inflammatory effects by modulating the NF-κB signaling pathway, thereby suppressing the production of inflammatory mediators. This study provides experimental evidence supporting the potential clinical use of EGS for inflammatory diseases.