본문 바로가기
KERD
논문KCI 등재

槐花散의 NF-κB 기전을 통한 항염증 효과 연구

Anti-inflammatory Effects of Goihwa-san Water Extract via NF-κB Inhibition

최지영|지선영|황보민|조현희
대한한방안이비인후피부과학회 (The Korean Medicine Opthalmology&Otolaryngology&Dermatology Society)|2023.02|한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology&Otorhinolaryngology&Dermatology)|제36권 제1호|pp. 21-39

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

NF-κBAnti-inflammatory effectGoihwa-san
주제분류한의학

초록

Objectives : The purpose of this study was to investigate the anti-inflammatory effect of Goihwa-san water extract(GHS) in vitro & in vivo. Methods : In vitro, we evaluated the anti-inflammatory effect of GHS by comparing the Raw 264.7 cells with 10, 30, 100, 300㎍/㎖ of GHS for 1 hour before Lipopolysaccharide(LPS) to the single LPS treated group. We examined the relative cell viability by MTT assay and the relative level of LPS, Loxoribine(LOX), Peptidoglycan(PGN), Flagellin(FLA)-induced NO production by using Griess reagent and measured relative iNOS protein level and COX-2 protein level by using western blot and Image analyzing system. We measured the production of TNF-α, IL-1β, and IL-6 by each ELISA kits and then measured the relative levels of IκBα, p-IκBα in whole-cell lysate fraction and NF-κB in nuclear fraction by using western blot and Image analyzing system. In vivo, we induced the paw edema by subcutaneous injection of 100㎕/rat CA and measured the swelling volume of paw by using a plethysmometer and then measured the relative iNOS protein level by using western blot. Results : As a result, in vitro, LPS, PGN-induced NO production was significantly inhibited by pretreatment with GHS. GHS reduced LPS, PGN-induced iNOS expression, PGN-induced COX-2 expression and LPS-induced production of cytokine(TNF-α, IL-1β, IL-6). Expression of IκBα was increased by pretreatment with GHS 100㎍/㎖. And the expression of p-IκBα and NF-κB were decreased by pretreatment with GHS 100㎍/㎖. In vivo, CA-induced inflammation rat model was used for the evaluation of the anti-inflammatory effect of GHS. 0.3 or 1.0g/㎏ of GHS significantly reduced the increases of paw swelling and iNOS expression in paw tissues. Conclusions : These results show that GHS can decrease inflammatory response via inhibition of the NF-κB pathway in vitro. And in vivo, the anti-inflammatory effect suggest the clinical basis of GHS for the treatment of inflammatory diseases.

Abstract

Objectives : The purpose of this study was to investigate the anti-inflammatory effect of Goihwa-san water extract(GHS) in vitro & in vivo. Methods : In vitro, we evaluated the anti-inflammatory effect of GHS by comparing the Raw 264.7 cells with 10, 30, 100, 300㎍/㎖ of GHS for 1 hour before Lipopolysaccharide(LPS) to the single LPS treated group. We examined the relative cell viability by MTT assay and the relative level of LPS, Loxoribine(LOX), Peptidoglycan(PGN), Flagellin(FLA)-induced NO production by using Griess reagent and measured relative iNOS protein level and COX-2 protein level by using western blot and Image analyzing system. We measured the production of TNF-α, IL-1β, and IL-6 by each ELISA kits and then measured the relative levels of IκBα, p-IκBα in whole-cell lysate fraction and NF-κB in nuclear fraction by using western blot and Image analyzing system. In vivo, we induced the paw edema by subcutaneous injection of 100㎕/rat CA and measured the swelling volume of paw by using a plethysmometer and then measured the relative iNOS protein level by using western blot. Results : As a result, in vitro, LPS, PGN-induced NO production was significantly inhibited by pretreatment with GHS. GHS reduced LPS, PGN-induced iNOS expression, PGN-induced COX-2 expression and LPS-induced production of cytokine(TNF-α, IL-1β, IL-6). Expression of IκBα was increased by pretreatment with GHS 100㎍/㎖. And the expression of p-IκBα and NF-κB were decreased by pretreatment with GHS 100㎍/㎖. In vivo, CA-induced inflammation rat model was used for the evaluation of the anti-inflammatory effect of GHS. 0.3 or 1.0g/㎏ of GHS significantly reduced the increases of paw swelling and iNOS expression in paw tissues. Conclusions : These results show that GHS can decrease inflammatory response via inhibition of the NF-κB pathway in vitro. And in vivo, the anti-inflammatory effect suggest the clinical basis of GHS for the treatment of inflammatory diseases.

참고문헌 (38)

  1. Jeon DH., "Silyoung Junguidaeyakgang", pp.630~ (2000), People’s Medical Publshing House Co
  2. Kim IR, "Bonchohak", pp.434~ (2007), Young Lim Publishing Co
  3. Sin MK, "Jeonghwa Imsangbonchohak", pp.499~ (2010), Young Lim Publishing Co
  4. Heo J., "Donguibogam", pp.138~140 (2005), Dongibogam-publishes
  5. The Whole Korean medicine Bonchohak professor Coedition, "Bonchohak", pp.434~ (2008), Yeonglimsa
  6. Kim CM, "Jungyakdaesajeon", pp.3983~ (1999), Jeongdam
  7. Moon GS, "Ingredients and Uses of Herbs", pp.362~ (1999), Ilwolbooks
  8. Seo BI, "The comprehensible herbology", pp.26~ (2004), Daegu Haany University Press
  9. 김정미, "Anti-platelet Effects of Flavonoids and Flavonoid-glycosides from Sophora japonica", Archives of Pharmacal Research, 31(7), pp.886~890 (2008), 대한약학회
  10. 배기상, "槐花 물 추출물의 항염증 효과", 동의생리병리학회지, 23(6), pp.1392~1398 (2009), 한의병리학회
  11. 김정미, "유방암세포에서 괴화 에탄올 추출물의 NF-kB 의존적인 MMP-9 발현의 조절 규명을 위한 연구", 동의생리병리학회지, 28(1), pp.22~28 (2014), 한의병리학회
  12. 박은영, "5종 단미제의 Staphylococcus aureus에 대한 in vitro 항균력 평가", 대한한방부인과학회지, 26(1), pp.25~40 (2013), 대한한방부인과학회
  13. Seo GE., "Isolation of Antioxidant from Roots of Sanguisorba officinalis", Department of Oriental Medicine Materials, pp.2015
  14. 박근혜, "지유(地楡) 부탄올 분획물의 Streptococcus mutans 항균활성 및 항산화 효과", 대한본초학회지, 27(3), pp.23~29 (2012), 대한본초학회
  15. 박진규, "담관암세포주에서 地楡 에탄올 추출물의 항암 효과", 대한한방내과학회지, 33(4), pp.465~475 (2012), 대한한방내과학회
  16. 황석연, "면역억제능을 보유한 地楡의 인슐린 분비능 연구", 동의생리병리학회지, 28(5), pp.499~505 (2014), 한의병리학회
  17. 이재준, "지유가 흰쥐의 급성위염 및 소화성 궤양에 미치는 영향", 한국식품영양과학회지, 34(10), pp.1545~1552 (2005), 한국식품영양과학회
  18. Lee SY, "Anxiolytic Activities of Sanguisorbae Radix", Yakhak Hoeji, 38(6), pp.733~741 (1994)
  19. 안봉전, "地楡(Sanguisorbae officinalis L.)의 항암 및 항균효과", Applied Biological Chemistry, 47(1), pp.141~145 (2004), 한국응용생명화학회
  20. Jeong HS, "Hemostatic effects of Sanguisorbae Radix and Nepetae Herba aqua-acupuncture", The journal of oriental Obstetrics & Gynecology, 13(1), pp.18~34 (2000)
  21. 배광호, "槐花散이 Dextran Sulfate Sodium으로 유도된 생쥐의 궤양성 대장염에 미치는 영향", 대한한방내과학회지, 31(3), pp.513~525 (2010), 대한한방내과학회
  22. Kang JC, "A Study of Goiwhasan’s Antigastric ulcer and Blood Hemostasis", Korea Oriental Medical Society, 19(1), pp.179~204 (1998)
  23. Song IS, "Inflammatory bowel disease in Korean", The Korean Association of Internal Medicine, 57(4), pp.661~674 (1999)
  24. Tiemey LM Jr, "Current Medical Diagnosis & Treatment", pp.686~695 (1999), Hanuri
  25. Cho YH., "Concepts of Human Physiology", pp.202~ (2011), Bummoon Education
  26. The Whole Korean medicine Gastroenterology professor Coedition, "Gastroenterology", pp.374~ (2008), Koonja publishing
  27. 박상미, "모려로부터 추출된 conchiolin의 LPS로 유도된 RAW 264.7 세포에서의 항염증 효과", 동의생리병리학회지, 22(4), pp.878~883 (2008), 한의병리학회
  28. Youm JH, "Role of Nitric Oxide in the Nickel and Cobalt Induced Cytotoxicity in RAW 264. 7 cell", Korean J Occup Environ Med, 13(3), pp.274~285 (2001)
  29. 김경효, "선천성 면역과 Toll Like Receptors", Clinical and Experimental Pediatrics, 47(1), pp.6~11 (2004), 대한소아청소년과학회
  30. Cari E, "Barrier-protective function of intestinal epithelial Toll-like receptor 2", Mucosal Immunology, 1, pp.62~66 (2008)
  31. Takeda K, "Toll receptors and pathogen resistance", Cell Microbiology, 5, pp.143~153 (2003)
  32. Lee BG, "Suppression of inducible nitric oxide synthase expression in Raw 264. 7 macrophages by two-carboline alkaloids extracted from Melia azedarach", Eur J Pharmacol, 406(3), pp.301~309 (2000)
  33. Wang MT, "Cycloxygenases, prostanoids and tumor progression", Cancer Metastasis, 26, pp.525~534 (2007)
  34. Hseu YC, "Anti-inflammatory potential of Antrodia Camphorata through inhibition of iNOS, COX-2 and cytokines via the NF-kappaB pathway", Intmmunopharmacol, 5(13, 14), pp.1914~1925 (2005)
  35. Min HS, "Human Physiology", pp.277~279 (2015), Yangseowon
  36. 신경민, "Amygdalin의 Murine Macrophage Raw 264.7 세포에서 in vitro 항염효과", 생약학회지, 34(3), pp.223~227 (2003), 한국생약학회
  37. Baeuerle PA, "IκB-NF-κB structures : at the interface of inflammation control", Cell, 95, pp.729~731 (1998)
  38. Dong C, "MAPK kinases in the immune response", Annual Review of Immunology, 20, pp.55~72 (2002)

관련 논문AI추천

金銀花散의 LPS로 유도된 Raw 264.7 세포에서의 항염증 효과김수진, 김연수, 지선영, 황보민대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2021 · 제34권 제3호NF-κB억제를 통한 淸心凉膈散의 항염증 효과김난이, 김연수, 지선영, 황보민대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2019 · 제32권 제2호LPS로 유도된 Raw 264.7 세포에서 銀翹散 열수 추출물의 항염증 효과이영은, 임지원, 하태인, 황보민대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2025 · 제38권 제2호Raw 264.7 세포에서 化毒丹의 항염증 효능에 대한 연구신기석, 지선영, 황보민대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2022 · 제35권 제3호甘露飮의 항염증 효과에 대한 실험적 연구김상찬, 임진호, 정호준, 지선영대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2010 · 제23권 제2호湯火止痛散의 NF-κB 및 MAPK 억제를 통한 항염증 효과고민정, 지선영, 황보민대한한방안이비인후피부과학회 · 한방안이비인후피부과학회지2023 · 제36권 제1호

이 간행물의 다른 논문 (8건)

湯火止痛散의 NF-κB 및 MAPK 억제를 통한 항염증 효과황보민, 고민정, 지선영pp. 1-20淸肺瀉肝湯의 피부 항노화 효과에 대한 연구김희택, 전보희, 송영채, 김용민pp. 40-49네트워크 약리학을 활용한 메니에르병에 대한 二陳湯의 활성 성분과 치료 기전 연구(Ⅰ)진선경, 남혜정pp. 50-63건성안 증후군에 대한 전자뜸과 침의 효과 비교 임상연구박수연, 송지훈, 최정화, 정민영, 박지식pp. 64-77Efficacy and Safety of a Biodegradable Hyaluronic Acid Microneedle Patch for Dry Skin in Atopic Dermatitis: A Single-blinded, Split-body, Randomized Controlled Trial이기현, 정민영, 박수연, 최정화, 송지훈pp. 78-95
원문 보기