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內托千金散이 RAW 264.7 대식세포주에서 항염증 활성에 미치는 영향

Effects of Naetakcheongeum-san on Anti-inflammatory Activities in RAW 264.7 cells

김희택|김용민|김태준
대한한방안이비인후피부과학회 (The Korean Medicine Opthalmology&Otolaryngology&Dermatology Society)|2018.02|한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology&Otorhinolaryngology&Dermatology)|제31권 제1호|pp. 12-21|DOI http://dx.doi.org/10.6114/jkood.2018.31.1.012

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키워드

PGE2Anti-inflammationiNOSCOX-2Naetakchunkeum-sanERK1/2
주제분류한의학

초록

Objectives : Inflammation is one of the self-protective abilities against tissue injury, and it has clinical symptoms like redness, heat, swelling, pain, and loss of function. The purpose of this study is to examine inhibitory effects of Naetakchunkeum-san (NTCKS) on nitric oxide (NO), Prostaglandin E2 (PGE2), inducible NOS (iNOS), cyclooxygenase-2 (COX-2), and phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2), which play a major role in inflammatory response. Methods : The experiment was performed using Raw 264.7 cells pretreated with NTCKS extracts. Cell viability was determined by MTT assay. To evaluate anti-inflammatory effects of NTCKS, we examined NO and PGE2 production in LPS-induced macrophages. We also investigated effects of NTCKS on iNOS, Cox-2, and ERK1/2 expression using western blot. Results : In MTT assay, no cytotoxicity of NTCKS (50, 100, 150, 200㎍/㎖) on RAW 264.7 cell was found. LPS-induced NO production was decreased after treatment with NTCKS (150, 200㎍/㎖)(p<0.05). PGE2 was decreased after treatment with NTCKS (150, 200㎍/㎖)(p<0.05). NTCKS inhibited LPS-induced expressions of iNOS and COX-2 in a dose-dependent manner. Increased phosphorylation of ERK1/2 by LPS was decreased by NTCKS in a dose-dependent manner. Conclusions : According to above experiments, NTCKS may be applied to inflammatory diseases such as atopic dermatitis, rheumatoid arthritis, and inflammatory bowel disease.

Abstract

Objectives : Inflammation is one of the self-protective abilities against tissue injury, and it has clinical symptoms like redness, heat, swelling, pain, and loss of function. The purpose of this study is to examine inhibitory effects of Naetakchunkeum-san (NTCKS) on nitric oxide (NO), Prostaglandin E2 (PGE2), inducible NOS (iNOS), cyclooxygenase-2 (COX-2), and phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2), which play a major role in inflammatory response. Methods : The experiment was performed using Raw 264.7 cells pretreated with NTCKS extracts. Cell viability was determined by MTT assay. To evaluate anti-inflammatory effects of NTCKS, we examined NO and PGE2 production in LPS-induced macrophages. We also investigated effects of NTCKS on iNOS, Cox-2, and ERK1/2 expression using western blot. Results : In MTT assay, no cytotoxicity of NTCKS (50, 100, 150, 200㎍/㎖) on RAW 264.7 cell was found. LPS-induced NO production was decreased after treatment with NTCKS (150, 200㎍/㎖)(p<0.05). PGE2 was decreased after treatment with NTCKS (150, 200㎍/㎖)(p<0.05). NTCKS inhibited LPS-induced expressions of iNOS and COX-2 in a dose-dependent manner. Increased phosphorylation of ERK1/2 by LPS was decreased by NTCKS in a dose-dependent manner. Conclusions : According to above experiments, NTCKS may be applied to inflammatory diseases such as atopic dermatitis, rheumatoid arthritis, and inflammatory bowel disease.

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