국내 밀 품종의 글리아딘 및 Spa 유전자 평가
Evaluation of Gliadin and Storage Protein Activator (Spa) in Korean Wheat Cultivars
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
국내 밀 품종의 가공적성 향상을 위하여 A-PAGE와 PCR을이용한 γ-와 ω-글리아딘과 밀 저장 단백질 발현에 관련 있는Spa 유전자를 평가하였다. 국내 40개 밀 품종들을 A-PAGE로분석한 결과, Gli-1 조성에 따라 13개 패턴으로 구분되었으며, Gli-A1, Gli-B1과 Gli-D1에서는 각각 7개, 5개와 6개 대립유전자들이 확인되었다. A-PAGE 분석 결과와 γ-글리아딘에 특이적으로 반응하는 마커들로 검정한 결과를 비교 분석한 결과, A-PAGE 분석에서 Gli-A1f, Gli-A1h와 Gli-A1l을 지닌 품종은GliA1.2마커에서만 반응하였고, Gli-B1h과 Gli-B1f 지닌 품종은GliB1.2마커에서만 반응하였으며, Gli-B1f, Gli-B1m과 Gli-B1o 를 지닌 품종은 모두 GliD1.1마커에서만 반응하였다. 나머지 대립유전자들은 GliD1.1과 GliD1.2마커에 반응하였다. γ-45와γ-42은 올밀과 중모2003을 제외하고 A-PAGE의 γ-글리아딘패턴이 일치하였다. 그러나 국내 밀 품종의 ω5-글리아딘을 분석한 결과 품종간에 차이가 없는 것으로 확인되었다. Spa 유전자를분석한 결과, 국내 밀 품종은 Spa-A1에서만 변이가 확인되었고, Spa-B1과 Spa-D1에서는 변이가 없는 것으로 확인되었다.
Abstract
Genetic variations of γ-/ω-gliadin and Spa (storage protein activator) in 40 Korean wheat cultivars were evaluated to provide genetic information for improving end-use quality in wheat breeding programs. Korean wheat cultivars were classified into 13 patterns at the Gli-1 locus based on the allelic variation using A-PAGE (acidic-polyacrylamide gel electrophoresis). Seven, five, and six alleles were identified at Gli-A1, Gli-B1, and Gli-D1 loci, respectively. Allele-specific PCR markers for γ-gliadin corresponded to specific allele at Gli-1 loci on A-PAGE, which Gli-A1f, Gli-A1h and Gli-A1l alleles corresponded to GliA1.2, Gli-B1h and Gli-B1f alleles corresponded to GliB1.2 and Gli-D1f, Gli-D1m and Gli-D1o alleles corresponded to GliD1.1. DNA markers for γ-45 and γ-42 also corresponded to the γ-gliadin patterns around 40kDa on A-PAGE, except in Sukang, Ol and Joongmo2003. However, allelic specific PCR markers for ω5-gliadin did not correspond to that of A-PAGE. Three alleles were identified at Spa-A1 locus, whereas there was no variation at Spa-B1 and Spa-D1 loci.
참고문헌 (31)
- Albani D, "The wheat transcriptional activator SPA : a seed-specific bZIP protein that recognizes the GCN4-like motif in the bifactorial endosperm box of prolamin genes", Plant Cell, 9, pp.171~184 (1997)
- Anderson O, "The gene space in wheat: the complete γ-gliadin gene family from the wheat cultivar Chinese spring", Funct. Integr. Genomics, 13, pp.261~273 (2013)
- Anderson OD, "The wheat ω-gliadin genes: structure and EST analysis", Fuct. Integr. Genomics, 9, pp.397~410 (2009)
- Barak S, "Biochemical and functional properties of wheat gliadins: A review", Crit. Rev. Food Nutr, 55, pp.357~368 (2015)
- Battais F, , Eur. Ann. Allergy Cln. Immunol, 40, pp.67~76 (2008)
- Branlard G, "Allelic diversity of HMW and LMW glutenin subunits and omega-gliadin in French bread wheat (Triticum aestivum L.)", Genet. Res. Crop Evol, 50, pp.669~679 (2003)
- Branlard G, "A null Gli-D1 allele with a positive effect on bread wheat quality", J. Cereal Sci, 20, pp.235~244 (1994)
- Büren M, "A spelt-specific γ-gliadin gene: discovery and detection", Theor. Appl. Genet, 100, pp.271~279 (2000)
- idio R, "PCR-based assay for detecting 1B-genes for low molecular weight glutenin subunits related to gluten quality properties in durum wheat", Plant Breed, 115, pp.413~415 (1996)
- Dupont FM, "Molecular and biochemical impacts of environmental factors on wheat grain development and protein synthesis", J. Cereal Sci, 38, pp.133~146 (2003)
- Eagle HA, "The storage protein activator gene Spa-B1 and grain quality traits in southern Australian wheat breeding programs", Crop Pasture Sci, 63, pp.311~318 (2012)
- Evrard A, "The promoter of the wheat puroindoline-a gene(PinA)exhibits a more complex pattern of activity than that of the PinB gene and is induced by wounding and pathogen attack in rice", Planta, 225, pp.287~300 (2007)
- Flaete NES, "Association between allelic variation at the combined Gli-1, Glu-3 loci and protein quality in common wheat (Triticum aestivum L.)", J. Cereal Sci, 37, pp.129~137 (2003)
- Guillaumie S, "Co-location between a gene encoding for the bZip factor SPA and an eQTL for a high-molecular-weight glutenin subunit in wheat(Triticum aestivum)", Genome, 47, pp.705~713 (2004)
- Gupta RB, "Allelic variation at glutenin subunit and gliadin loci, Glu-1, Glu-3, and Gli-1, of common wheats. II. Biochemical basis of the allelic effects on dough properties", J Cereal Sci, 19, pp.19~29 (1994)
- Matsuo H, "Molecular cloning, recombinant expression and IgE-binding epitope of ω-5 gliadin, a major allergen in wheat-dependent exercise-induced anaphylaxis", FEBS J, 272, pp.4431~4438 (2005)
- Metakovsky EV, "Relationship between gliadin allele and dough strength in Italian bread wheat cultivars", J. Cereal Sci, 25, pp.229~236 (1997)
- Metakovsky EV, "High genetic diversity of Spanish common wheats as judged from gliadin alleles", Plant Breed, 119, pp.37~42 (2000)
- Metakovsky EV, "Gliadin allele identifcation in common wheat. II. Catalogue of gliadin alleles in common wheat", J. Genet. Breed, 34, pp.325~344 (1991)
- Park CS, "Influence of allelic variations in glutenin on the quality of pan bread and white salted noodles made from Korean wheat cultivars", Euphytica, 180, pp.235~250 (2011)
- Qi PF, "Biochemical and molecular characterization of gliadins", Mol. Biol, 40, pp.713~723 (2006)
- Rasheed A, "Wheat seed storage proteins: Advances in molecular genetics, diversity and breeding applications", J. Cereal Sci, 60, pp.11~24 (2014)
- Ravel C, "Nucleotide polymorphism in the wheat transcriptional activator Spa influences its pattern of expression and has pleiotropic effects on grain protein composition, dough Spa-B1 gene effects in wheat viscoelasticity, and grain hardness", Plant Physiol, 151, pp.2133~2144 (2009)
- Ruiz M, "Polymorphism, variation and genetic identity of Spanish common wheat germplasm based on gliadin alleles", Field Crops Res, 79, pp.185~196 (2002)
- Salse J, "New insights into the origin of the B genome of hexaploid wheat : Evolutionary relationships at the SPA genomic region with the S genome of the diploid relative Aegilops speltoides", BMC Genomics, 9, pp.555~ (2008)
- Shewry PR, "Cereal seed storage proteins: structures, properties and role in grain utilization", J. Exp. Bot, 53, pp.947~958 (2002)
- Tatham AS, "The S-poor prolamins of wheat, barley and rye: revisited", J. Cereal Sci, 55, pp.79~99 (2014)
- Wang A, "Characterization of two 1D-encoded ω-gliadin subunits closely related to dough strength and pan bread-making quality in common wheat (Triticum aestivum L.)", J. Cereal Sci, 47, pp.528~535 (2008)
- Wu F, "Unique gliadin patterns in Chinese winter wheat cultivars", Plant Breed, 126, pp.498~502 (2007)
- Yildirim A, "Marker-assisted breeding of a durum wheat cultivar for γ-gliadin and LMW-glutenin proteins affecting pasta quality", Turk. J. Agric. For, 37, pp.527~533 (2013)
- Zhang W, "Identification of SNPs and development of allele-specific PCR markers for γ-gliadin alleles in Triticum aestivum", Theor. Appl. Genet, 107, pp.130~138 (2003)