백진주의 저아밀로스 유전분석 및 분자지도 작성
Genetic Analysis and Molecular Mapping of Low Amylose Gene in Rice Cultivar ‘Baegjinju’ (Oryza sativa L.)
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초록
쌀을 식량생산 위주의 1차 산업으로만 간주하던 시각을 가공, 소비, 연관 산업을 포함하는 통합적인 관점에서 인식의 전환 및 새로운 가치 창출이 절실히 요구되고 있다. 쌀에서 다양한 전분특성을 가진 품종 육성을 위해서 ‘백진주’의 dull 유전자에 대한 유전학적인 연구를 수행하였다. ‘새일미’와 ‘백진주’의 F2 집단에서 dull형과 translucent형은 각각 62개, 218개로서 1 : 3으로 분리하였으며 1개의 열성유전자에 의해 유전됨을 확인하였다. ‘백진주’의 저아밀로스 유전자는 10번 염색체의 장완에 RM6704와 RM25676사이에 존재하며, 물리적인 위치는 17.5Mb ～ 18.8Mb 사이에 존재하였다. 본 연구에서는 ‘백진주’의 저아밀로스 유전자와 가장 밀접히 연관된 SSR markerRM25648를 선발하였으며, 금후 MAS를 이용한 ‘백진주’의 저아밀로스 유전자 선발에 활용될 것으로 기대된다
Abstract
The amylose contents of rice determine eating quality which is one of the major traits in rice breeding program. To identify the low-amylose gene of the japonica rice cultivar Baegjinju, genetic analysis was conducted using 200 F2 population derived from a cross between the japonica cultivars, Saeilmi and Baegjinju. Individual F2 plants were classified as wild type (translucent grain) and mutant type (dull grain) based on the grain appearance of brown rice. Two hundred F2 plants were segregated into 155 wild type plants and 45 mutant type plants, which fit the 3:1 ratio (χ2 = 0.667, df = 1, p = 0.414) and this result indicated the low-amylose gene of Baegjinju is a single recessive gene which controls the amylose contents. Linkage analysis was conducted to localize the low-amylose gene of Baegjinju and fine mapped within an 800-kb interval between 17.5 to 18.8Mb on short arm of chromosome 10. Co-segregated SSR marker, RM25648 was developed and it could be useful for marker-assisted selection and determination of the genetic resource related with amylose contents in rice breeding.
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