PCR 마커를 이용한 국내 밀 품종의 고분자 글루테닌 Glu-B1 대립유전자 조성 평가
Assessment of Allele Composition of HMW-GS Glu-B1 Locus in Domestic Wheat Cultivars Using PCR-based Markers
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초록
In common wheat (Triticum aestivum L.), the protein content and glutenin proteincomposition are the key quality-determining parameters. Allelic variations, especially inhigh-molecular-weight glutenin subunits (HMW-GSs), affect bread quality significantly. The HMW-GS Glu-1 locus consists of two tightly linked genes encoding x- and y-typesubunits that exhibit highly variable frequencies. In this study, we evaluated Glu-B1 allelesusing allele-specific PCR markers in 44 domestic wheat cultivars. The composition ofGlu-1Bx7+Glu-1By8 in the 24 cultivars was either Glu-1Bx7+Glu-1By8, Glu-1Bx7*+Glu-1By8, or Glu-1Bx7*+Glu-1Bx8*. In addition, the two cultivars initially identified Glu-1Bx7+Glu-1By8* were corrected to Glu-1Bx7*+Glu-1By8*. Seven cultivars previouslyclassified as having Glu-1Bx7+Glu-1By9 composition contained Glu-1Bx7*+ Glu-1Bx9. The allele composition of the cultivar was identified as Glu-1Bx20+Glu-1By20 instead ofGlu-1By20. The HMW-GSs of 21 wheat varieties were analyzed using ultra-performanceliquid chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These results will be helpful for evaluating the composition of Glu-B1 alleles in domesticwheat and accurately assessing the quality of domestic wheat flour.
Abstract
In common wheat (Triticum aestivum L.), the protein content and glutenin proteincomposition are the key quality-determining parameters. Allelic variations, especially inhigh-molecular-weight glutenin subunits (HMW-GSs), affect bread quality significantly. The HMW-GS Glu-1 locus consists of two tightly linked genes encoding x- and y-typesubunits that exhibit highly variable frequencies. In this study, we evaluated Glu-B1 allelesusing allele-specific PCR markers in 44 domestic wheat cultivars. The composition ofGlu-1Bx7+Glu-1By8 in the 24 cultivars was either Glu-1Bx7+Glu-1By8, Glu-1Bx7*+Glu-1By8, or Glu-1Bx7*+Glu-1Bx8*. In addition, the two cultivars initially identified Glu-1Bx7+Glu-1By8* were corrected to Glu-1Bx7*+Glu-1By8*. Seven cultivars previouslyclassified as having Glu-1Bx7+Glu-1By9 composition contained Glu-1Bx7*+ Glu-1Bx9. The allele composition of the cultivar was identified as Glu-1Bx20+Glu-1By20 instead ofGlu-1By20. The HMW-GSs of 21 wheat varieties were analyzed using ultra-performanceliquid chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These results will be helpful for evaluating the composition of Glu-B1 alleles in domesticwheat and accurately assessing the quality of domestic wheat flour.
참고문헌 (34)
- Ahmad M, "Molecular marker-assisted selection of HMW glutenin alleles related to wheat bread quality by PCR generated DNA markers", Theor Appl Genet, 101, pp.892~889 (2000)
- Brankovic G, "Genetic parameters of Triticum aestivum and Triticum durum for technological quality properties in Serbia", Zemdirbyste, 105, pp.39~48 (2018)
- Butow BJ, "Molecular discrimination of Bx7 alleles demonstrates that a highly expressed high molecular-weight glutenin allele has a major impact on wheat flour dough strength", Theor Appl Genet, 107, pp.1524~1532 (2003)
- Espi A, "A PCR-based method for discriminating between high molecular weight glutenin subunits Bx7 and Bx7* in Triticum aestivum L", Plant breed, 131, pp.571~573 (2012)
- Frank K, "Development of a PCR-based DNA marker for Glu-1By alleles in the old Hungarian Bankuti wheat", Mol Breeding, 37, pp.1~5 (2017)
- Gao L, "Characterization and comparative analysis of wheat high molecular weight glutenin subunits by SDS-PAGE, RP-HPLC, HPCE, and MALDI-TOF-MS", J Agric Food Chem, 58, pp.2777~2786 (2010)
- Geng Y, "Expression of wheat high molecular weight glutenin subunit 1Bx is affected by large insertions and deletions located in the upstream flanking sequences", PLoS One, 9, pp.e105363~ (2014)
- Gianibelli MC, "Polymorphism of high Mr glutenin subunits in Triticum tauschii : characterisation by chromatography and electrophoretic methods", J Cereal Sci, 33, pp.39~52 (2001)
- Heo H, "Identification of QTL for grain protein content and grain hardness from winter wheat for genetic improvement of spring wheat", Plant Breed Biotech, 1, pp.347~353 (2013)
- Jang YR, "Improved method for reliable HMW-GS identification by RP-HPLC and SDS-PAGE in common wheat cultivars", Molecules, 22, pp.1055~ (2017)
- Jang YR, "Development of an optimized MALDI-TOF-MS method for high-throughput identification of high-molecular-weight glutenin subunits in wheat", Molecules, 25, pp.4347~ (2020)
- Lee JY, "Two-dimensional electrophoresis of high molecular weight glutenin subunits in Korean wheat cultivars", Korean J Breed Sci, 45, pp.240~252 (2013)
- Lee YG, "Genomics approach to identify the cause of the missing omega-5gliadin protein in O-free wheat", Plant Breed Biotech, 6, pp.413~425 (2018)
- Lee MH, "Analysis of protein properties and gluten protein composition evaluation of wheat genetic resources", Korean J Breed Sci, 54, pp.245~259 (2022)
- Lee MH, "Generation of wheat near-isogenic lines overexpressing 1Bx7 glutenin with increased Protein contents and SDS-sedimentation values", Plants, 12, pp.1244~ (2023)
- Lee MH, "Development of PCR-based markers for the identification of wheat HMW glutenin Glu-1Bx and Glu-1By alleles", BMC Plant Biol, 24, pp.395~ (2024)
- Lei ZS, "Y-type gene specific markers for enhanced discrimination of high molecular weight glutenin alleles at the Glu-B1 locus in hexaploid wheat", J Cereal Sci, 43, pp.94~101 (2006)
- Liu S, "New DNA markers for high molecular weight glutenin subunits in wheat", Theor Appl Genet, 118, pp.177~183 (2008)
- Ma W, "Multiplex-PCR typing of high molecular weight glutenin alleles in wheat", Euphytica, 134, pp.51~60 (2003)
- Park CS, "Influence of allelic variations in glutenin on the quality of pan bread and white salted noodles made from Korean wheat cultivars", Euphtica, 180, pp.235~250 (2011)
- Ragupathy R, "Evolutionary origin of the segmental duplication encompassing the wheat Glu-B1 locus encoding the overexpressed Bx7(Bx7OE)high molecular weight glutenin subunit", Theor Appl Genet, 116, pp.283~296 (2008)
- Agricultural science and technology of analysis based on research(Ⅰ), "RDA(Rural Development Administration)", 315~374 (2012)
- Schwarz G, "Development and validation of a PCR-based marker assay for negative selection of the HMW GluB1-1d(Bx-6)in wheat", Theor Appl Genet, 109, pp.1064~1069 (2004)
- Shewry PR, "High molecular weight subunits of wheat glutenin", J Cereal Sci, 15, pp.105~120 (1992)
- Shin D, "Validation and selection of functional allele-specific molecular markers to analyze High-Molecular-Weight glutenin subunit composition in wheat", Korean S Breed Sci, 52, pp.235~243 (2020)
- Skeritt JH, "Gluten proteins : genetics, structure and dough quality-a review", AgBiotech News an Information, 10, pp.247N~270N (1998)
- Smith RL, "Identification of glutenin alleles in wheat and triticale using PCR-generated DNA markers", Crop Sci, 34, pp.1373~1378 (1994)
- Vyhnanek T, "Glu-1 alleles and prediction of bread-making quality traits in triticale", Cereal Res Commun, 41, pp.562~572 (2013)
- Wan Y, "High-molecular-weight glutenin subunits in the Cylindropyrum and Vertebrata section of the Aegilops genus and identification of subunits related to those encoded by the Dx alleles of common wheat", Theor Appl Genet, 101, pp.879~884 (2000)
- Wang S, "Molecular mechanisms of HMW glutenin subunits from 1Sl genome of Aegilops longissima positively affecting wheat bread making quality", PLoS One, 8, pp.e58947~ (2013)
- Xu Q, "PCR-based markers for identification of HMW-GS at Glu-B1x loci in common wheat", J Cereal Sci, 47, pp.394~398 (2008)
- Yan Y, "Allelic variation of the HMW glutenin subunits in Aegilops tauschii accessions detected by sodium dodecyl sulphate(SDS-PAGE), acid polyacrylamide gel(A-PAGE)and capillary electrophoresis", Euphytica, 130, pp.377~385 (2003)
- Yu Z, "Rapid characterization of wheat low molecular weight glutenin subunits by ultraperformance liquid chromatography(UPLC)", J Agric Food chem, 61, pp.4026~4034 (2013)
- Zhang Q, "Characterization of HMW glutenin subunits in common wheat and related species by matrix-assisted laser desorption", ionization time-of-flight mass spectrometry(MALDI-TOF-MS), 2008, pp.47, 252~261
