국내 밀 품종의 2차원 전기영동 분석을 통한 고분자 글루테닌 분석
Two-dimensional Electrophoresis of High Molecular Weight Glutenin Subunits in Korean Wheat Cultivars
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
To evaluate qualitative and quantitative expression level of HMW-GS protein, we separated glutenin fractions and conducted two-dimensional electrophoresis (2DE) in 32 Korean wheat cultivars. The average spot numbers of HMW-GS in Korean wheat cultivars were 11.78 which included 1.31, 5.53 and 4.94 spots at Glu-A1, Glu-B1 and Glu-D1 loci, respectively. Cultivars harboring 1, 2* subunits had more spots than ones with null allele in Glu-A1 locus because Glu-B1 and Glu-D1 loci did not show difference in spot number. A large variation in the total spot number of HMW-GS was observed ranging from 7 (Dahong) to 18 (Jokyung). Relative expression level of Korean wheat cultivars when compared with the check accession Chinese Spring ranged from 24% (Eunpa) to 111% (Gobun) with an average of 44%. Korean wheat accessions could be classified into six groups based on the number of HMW-GS spots and quantification value of each spot.
Abstract
To evaluate qualitative and quantitative expression level of HMW-GS protein, we separated glutenin fractions and conducted two-dimensional electrophoresis (2DE) in 32 Korean wheat cultivars. The average spot numbers of HMW-GS in Korean wheat cultivars were 11.78 which included 1.31, 5.53 and 4.94 spots at Glu-A1, Glu-B1 and Glu-D1 loci, respectively. Cultivars harboring 1, 2* subunits had more spots than ones with null allele in Glu-A1 locus because Glu-B1 and Glu-D1 loci did not show difference in spot number. A large variation in the total spot number of HMW-GS was observed ranging from 7 (Dahong) to 18 (Jokyung). Relative expression level of Korean wheat cultivars when compared with the check accession Chinese Spring ranged from 24% (Eunpa) to 111% (Gobun) with an average of 44%. Korean wheat accessions could be classified into six groups based on the number of HMW-GS spots and quantification value of each spot.
참고문헌 (40)
- AlberGhina G, "Proteomics of gluten: mapping of the 1Bx7 glutenin subunit in Chinese Spring", Theor. Rapid Commun. Mass Spectrom, 19, pp.2069~2074 (2005)
- An X, "Cloning and molecular characterization of three novel LMW-i glutenin subunit genes from cultivated einkorn", Theor. Appl. Genet, 113, pp.383~395 (2006)
- Bean SR, "High-permormance capillary electrophoresis of cereal proteins", J. Chromatogr, 814, pp.25~41 (1998)
- Belton PS, "On the elasticity of wheat gluten", J. Cereal Sci, 29, pp.103~107 (1999)
- Belton PS, "FTIR and NMR studies on the hydration of a high Mr subunit of glutenin", Int. J. Biol. Macromol, 17, pp.74~ (1995)
- Bietz JA, "Separation of cereal proteins by reversedphase high-performance liquid chromatography", J. Chromatogr, 255, pp.219~238 (1983)
- Branlard G, "Allelic diversity of HMW and LMW glutenin subunits and omega-gliadins in French bread wheat", Genet. Res. Crop Evol, 50, pp.669~679 (2003)
- Bradford MM, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", Anal. Biochem, 72, pp.248~254 (1976)
- Chen J, "Matrix-assisted laser desorption", ionization timeof- flight based wheat gliadin protein peaks are useful molecular markers for wheat genetic study, 2007, pp.21, 2913~2917
- Cozzolino R, "Matrix-assisted laser desorption", ionization mass spectrometric peptide mapping of high molecular weight glutenin subunits 1Bx7 and 1Dy10 in Cheyenne cultivar. Rapid Commun. Mass Spectrom, 2001, pp.15, 778~787
- Cozzolino R, "Proteomics of gluten: mapping of subunit 1 Ax2* in Cheyenne cultivar by matrix-assisted laser desorption", ionization, 2001, pp.15, 1129~1135
- Cunsolo V, "Structural studies of glutenin subunits 1Dy10 and 1Dy12 by matrix-assisted laser desorption", ionisation mass spectrometry and high-performance liquid chromatography, Rapid Commun. Mass Spectrom, pp.2003, 17[DOI]
- Courcoux P, "Characterization and identification of wheat cultivars by multi-dimensional analysis of reversed-phase high-performance liquid chromatograms", J. Chromatogr, 596, pp.225~235 (1992)
- Feeney KA, "Molecular structures and interactions of repetitive peptides based on wheat glutenin subunits depend on chain length", Biopolym. Biospectrosc, 72, pp.123~131 (2003)
- Foti S, "Verification of the cDNA deduced sequence of glutenin subunit 1Dx5 and an Mr 58000 repetitive peptide by matrix-assisted laser desorption", ionisation mass spectrometry, 2000, pp.31, 173~183
- Garozzo D, "Use of hydroxyacetophenones as matrices for the analysis of high molecular weight glutenin mixtures by matrix-assisted laser desorption", ionization mass spectrometry, 1999, pp.13, 2084~2089
- Gianibelli MC, "Biochemical, genetic, and molecular characterization of wheat glutenin and its component subunits", Cereal Chem, 78, pp.635~646 (2001)
- Graybosch RA, "High molecular weight glutenin subunit composition of cultivars, germplasm, and parents of US red winter wheat", Crop Sci, 32, pp.1151~1155 (1992)
- Hong BH, "Genetic variation of high molecular weight glutenin subunit in Korea wheat", Korean J. Crop Sci, 43, pp.259~263 (1998)
- Liu K, "PowerMarker: an integrated analysis environment for genetic marker analysis", Bioinformatics, 21, pp.2128~ (2005)
- Liu L, "MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding", J. Cereal Sci, 50, pp.295~301 (2009)
- Nei M., "The theory and estimation of genetic distance, In Genetic Structure of Populations", pp.45~54 (1973), University Press
- Oda S, "Relation between starch granule protein and endosperm softness in Japanese wheat cultivars", J. Breed, 42, pp.161~165 (1992)
- Payne PI, "Genetics of wheat storage proteins and the effect of allelic variation on readmaking quality", Ann. Rev. Plant Physiol, 38, pp.141~153 (1987)
- Payne PI, "Catalogue of alleles for the complex gene loci Glu-A1, Glu-B1 and Glu-D1 which code for high-molecular weight subunits of glutenin in hexaploid wheat", Cereal Res. Commun, 11, pp.29~35 (1983)
- Payne PI, "Detection of a novel high molecular weight subunit of glutenin in some Japanese wheats", J. Cereal Sci, 1, pp.3~8 (1983)
- Payne PI, "Allelic variation of glutenin subunits and gliadins and its effect on bread making quality in wheat: analysis of F5 progeny from Chinese Spring", J. Cereal Sci, 6, pp.103~118 (1987)
- 박철수, "Allelic Variation of Glutenin, Granule-Bound Starch Synthase I and Puroindoline in Korean Wheat Cultivar", 한국작물학회지, 54(2), pp.181~191 (2009), 한국작물학회
- Jung-Dae Lim, "Flour Characteristics and End-Use Quality of Korean Wheats with 1Dx2.2+1Dy12 Subunits in High Molecular Weight Glutenin", Preventive Nutrition and Food Science, 11(3), pp.243~252 (2006), 한국식품영양과학회
- Park CS, "Influence of allelic variations in glutenin on the quality of pan bread and white salted noodles made from Korean wheat cultivars", EUPHYTICA, 180(2), pp.235~250 (2011), SPRINGER
- Payne PI, "Identification of a high-molecular-weight subunit of glutenin whose presence correlates with bread-making quality in wheats of related pedigree", Theor. Appl. Genet, 55, pp.53~159 (1979)
- Popineau Y, "Influence of high Mr glutenin subunits on glutenin polymers and rheological properties of gluten and gluten subfractions of near-isogenic lines of wheat Sicco", J. Cereal Sci, 19, pp.231~241 (1994)
- Redaelli R, "Allelic variation at the storage protein loci of 55 US-grown white wheats", Plant Breed, 116, pp.429~436 (1997)
- Roels SP, "Evidence for the nonglycoprotein nature of high molecular weight glutenin subunits of wheat", J. Cereal Sci, 24, pp.227~239 (1996)
- Shewry PR, "High molecular weight subunits of wheat glutenin", J. Cereal Sci, 15, pp.105~120 (1992)
- Singh NK, "A simplified SDS-PAGE procedure for separating LMW subunits of glutenin", J. Cereal Sci, 14, pp.203~208 (1990)
- Tamura K, "MEGA4: molecular evolutionary genetics analysis software version 4.0", Mol. Biol. Evol, 24, pp.1596~ (2007)
- Yahata E, "Relationship between the dough quality and content of specific glutenin proteins inwheatmill streams,andits application tomakingflour suitable forinstant Chinese noodles", Biosci Biotechnol. Biochem, 70, pp.788~797 (2006)
- Yeboah NA, "Fluorescence studies of two γ-gliadin tractions from bread wheat", J. Cereal Sci, 19, pp.141~148 (1994)
- Zhang Q, "Characterization of HMW glutenin subunits in common wheat and related species by matrix- assisted laser desorption", ionisation time-of-flight mass spectrometry, 2008, pp.47, 252~261
