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調經種玉湯이 Immobilization Stress를 負荷한 C57BL/6J 생쥐의 免疫系에 미치는 影響

Effects of Chokyungjongoktang on Immune System by ImmobilizationStress in C57BL/6J Mice

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Abstract

The purpose of this research was to investigate the effects of Chokyungjongoktang water extract(CJT) on immune system by immobilization stress in C57BL/6J mice. CJT decreased the serum level of histamine and corticosterone increased by immobilization stress. In addition, CJT did not affect the viability of thymocytes and enhanced the viability of splenocytes decreased by immobilization. Also, CJT increased the viability of thymocytes and decreased the viability of splenocytes in vitro. CJT did not affect DNA fragmentation of thymocytes and splenocytes increased by immobilization stress. CJT increased the population of thymic CD4+ cells increased by immobolization stress, and decreased the population of Thy1+ cells increased by immobilization stress and increased the population of splenic CD8+ cells increased by immobilization stress. CJT enhenced the production of -interferon and IL-2 decreased by immobilization stress. Also, CJT decreased the phagocytic activity decreased by immobilization stress and decreased the level of nitric oxide decreased by immobilization stress. These results indicate that CJT may be useful for the prevention and treatment of stress via suppression of serum histamine and corticosterone level, and enhancement of viability of splenocytes and cytokines production from Th1 cells in splenocytes.

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