사례연구: 16S ribosomal RNA 유전자의 초가변영역(V1-V4)을 활용한 미생물 동정 사례
Case Study : Microbial Identification using V1-V4 Hypervariable Region of 16S ribosomal RNA gene
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초록
미생물 종 동정을 위한 분자학적 방법으로는 16S ribosomal RNA (rRNA) 유전자의 염기서열 분석이 대표적으로 이용된다 . 본 연구에서는 16S rRNA 유전자의 초가변영역 중 V1-V3 영역의 염기서열 분석을 통하여 종 (species) 을 동정하는 상용화된 미생물 종 동정 키트인 Fast MicroSEQ TM 500 16S rDNA Bacterial Identification System 의 사용으로 인해 빈번히 발생하는 종 동정의 어려움을 개선하고자 하였다 . 이를 위해 분석하는 염기서열의 길이를 V1-V4 영역으로 확장하고 , EzBioCloud 16S-based ID 를 데이터베 이스로 추가 활용하여 미생물 종 동정능이 향상된 사례를 소개하고자 한다 .
Abstract
16S ribosomal RNA (rRNA) gene has been used as a conventional method for microbial identification in molecular biology. In this study, it has tried to solve some limitations in microbial identification in the species level when using a commercial kit, Fast MicroSEQ TM 500 16S rDNA Bacterial Identification System, which covers V1-V3 region. We made the sequences of the samples lengthened to cover V1-V4 region, and applied an additional database named EzBioCloud 16S-based ID to increase the discriminating power.
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