무산소성 세균 동정을 위한 Vitek 2와 API 20A,16s rRNA 유전자 염기서열 분석법의 비교
Comparison of the Vitek 2, API 20A, and 16s rRNA Gene Sequencing for the Identification of Anaerobic Bacteria
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배경: 최근 무산소성 균주를 정확하고 빠르게 동정하기 위해 기존의 표현형 검사방법을 대체하는 유전자형 검사방법의 사용이 증가하는 추세이다. 본 연구에서는 Vitek 2, API 20A와 16s rRNA 유전자 염기서열 분석법의 무산소성 균주에 대한 동정결과를 평가해보았다. 방법: Vitek 2, API 20A, 16s rRNA 유전자 염기서열 분석법을 사용하여 무산소성 표준균주 35주를 동정하였다. 3가지 검사법의 정확한 동정률을 비교하여 각 검사법의 동정능을 평가하였다. 결과: Vitek 2, API 20A, 16s rRNA 유전자 염기서열 분석법은 종수준에서 각각 54.3%, 15.4%, 94.3%를 정확하게 동정하였으며, 속수준에서 77.1%, 42.3%, 100%를 정확하게 동정하였다. McNemar's test 결과, 속수준에서의 동정결과에서 3가지 검사법 간에 통계적으로 유의한 차이를 보였다(P value＜0.05). 결론: 16s rRNA 유전자 염기서열 분석법은 무산소성 세균의 동정에 대하여 Vitek 2, API 20A보다 더 정확한 동정결과를 나타냈다. 표현형적 동정 시스템으로 정확한 무산소성 세균 동정이 어려운 경우 16s rRNA 유전자 염기서열 분석법이 정확한 동정에 도움이 될 것으로 보인다. [Ann Clin Microbiol 2015;18:20-26]
Abstract
Background: Recently, genotypic identification of anaerobes is emerging as an alternative to the phenotypic method. In this study, we evaluated the performance of Vitek 2, API 20A and 16s rRNA gene sequencing for the identification of anaerobic bacteria. Methods: A total of 35 anaerobe reference strains were identified using Vitek 2, API 20A and 16s rRNA gene sequencing. We evaluated the performance of three methods on the basis of the accurate identification rates.Results: The Vitek 2, API 20A and 16s rRNA gene sequencing identified 54.3, 15.4, and 94.3% of test strains correctly at the species level and identified 77.1, 42.3, and 100% at the genus level, respectively. Results of the McNemar's test showed that there was a significant difference between each of the three identification methods in species level identification (P value＜0.05).Conclusion: 16s rRNA gene sequencing showed better performance than Vitek 2 or API 20A for anaerobic bacteria. Considering its excellent performance, 16s rRNA gene sequencing may be useful for accurate identification of anaerobic bacteria that cannot be correctly identified by phenotypic methods. (Ann Clin Microbiol 2015;18:-26)
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