강원도지역 블루베리 잎에서 분리한 Diaporthe spp.의 동정과 병원성 검정
Identification and Pathogenicity of Diaporthe spp. from Blueberry Leaf in Gangwon Province
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Diaporthe속은 전세계적으로 중요한 곰팡이 병원균 중 하나이다. 블루베리에 발생하는 병에 관한연구 결과 강원도 춘천시와 고성시의 블루베리농가에서 5균주의 Diaporthe속을 분리하였다. 이들의 형태적 특징과 분자생물학적 특징을 조사한결과 Diaporthe amygdali, D. eres, D. nobilis, D. phaseolorum, Diaporthe sp.로 동정되었다. 병원성 검정결과 Diaporthe amygdali, D. nobilis, D. phaseolorum 은 상처를 낸 잎에서 5~10mm의병반을 형성하였다. Diaporthe eres는 상처낸 잎에서 10mm 이상의 병반을 형성하였고 Diaporthe sp.는 상처를 낸 잎에서 5mm 이하의 병반을 형성하였다. 하지만 상처를 내지 않은 잎과 과실에서는 병반을 확인할 수 없었다. 온도 생장특성은 D. eres, D. nobilis, D. phaseolorum, Diaporthe sp.는 25℃에서D. amygdali는 30℃에서 가장 높은 생장을 보였다. 모든 분리균들은 oxine-copper, benomyl, fluzinam, prochloraz manganese complex에서 80%이상의 생장저해율을 보였고 captan과 mancozeb는 250ppm, 500ppm에서 dithianon는 500ppm에서 80%이상의 생장저해율을 보였다.
Abstract
The genus Diaporthe is one of the important fungal pathogens in the world. 5 isolates were obtained from blueberry leaves in Chuncheon and Gosung during a survey on blueberry disease. From the results identification using morphology and molecular characteristics, they were identified as Diaporthe amygdali, D. eres, D. nobilis, D. phaseolorum and Diaporthe sp. Diaporthe amygdali, D. nobilis and D. phaseolorum developed lesion of 5~10mm in diameter when inoculated onto leaves with wounds. And D. eres and Diaporthe sp. developed over than 10mm and less than 5mm lesion, repectively. But didn’t develop onto leaves without wounds and fruit with/without wounds. Fungal growth at various temperatures showed that D. eres, D. nobilis, D. phaseolorum, and Diaporthe sp. had the highest growth rates at 25℃, while D. amygdali grew better at 30℃. All strains showed 80% or higher inhibited growth in all concentrations of oxine-copper, benomyl, fluzinam, and prochloraz manganese complex. They also showed 80% or higher inhibited growth in 250 and 500 ppm of captan, 250 and 500 ppm of mancozeb, and 500ppm of dithianon.
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