Investigation of Phenotypic and Genetic Variation in Colletotrichum acutatum Isolates in South Korea
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Colletotrichum acutatum J. H. Simmonds is an important fungal pathogen that causesanthracnose disease in crops. In this study, 59 C. acutatum isolates from regions in SouthKorea were collected and phenotypically compared. The average colony diameters of isolatesfrom Gyeongsangbuk-do, Chungcheongbuk-do, and Gangwon-do were 4.36 cm, 4.24 cm, and4.38 cm, respectively, following 6 days of growth on V8 juice agar. The average conidiumconcentrations of the isolates from each province were 14.43, 12.83, and 10.17 × 104/mL after 7days of growth on V8A. Amplified fragment length polymorphism(AFLP) analysis using threeprimer pairs identified 5 polymorphic bands and 56 monomorphic bands. Two polymorphicbands were identified using the E-ACC/M-CTT primer pair, one polymorphic band wasidentified using the E-ACG/M-CTG primer pair, and two polymorphic bands were identifiedusing the E-ACG/M-CTT primer pair. A dendrogram was constructed using the AFLP data, andfour clusters were identified.
Abstract
Colletotrichum acutatum J. H. Simmonds is an important fungal pathogen that causesanthracnose disease in crops. In this study, 59 C. acutatum isolates from regions in SouthKorea were collected and phenotypically compared. The average colony diameters of isolatesfrom Gyeongsangbuk-do, Chungcheongbuk-do, and Gangwon-do were 4.36 cm, 4.24 cm, and4.38 cm, respectively, following 6 days of growth on V8 juice agar. The average conidiumconcentrations of the isolates from each province were 14.43, 12.83, and 10.17 × 104/mL after 7days of growth on V8A. Amplified fragment length polymorphism(AFLP) analysis using threeprimer pairs identified 5 polymorphic bands and 56 monomorphic bands. Two polymorphicbands were identified using the E-ACC/M-CTT primer pair, one polymorphic band wasidentified using the E-ACG/M-CTG primer pair, and two polymorphic bands were identifiedusing the E-ACG/M-CTT primer pair. A dendrogram was constructed using the AFLP data, andfour clusters were identified.
참고문헌 (29)
- Ansari KI, "Pathogenic and genetic variability among Colletotrichum lindemuthianum isolates of different geographic origins", Plant Pathol, pp.635~642 (2004)
- Bailey JA, "Colletotrichum: biology, pathology and control", British Society for Plant Pathology, pp.388~ (1992)
- Chala A, "Genetic differentiation and gene flow in Colletotrichum sublineolum in Ethiopia, the centre of origin and diversity of sorghum, as revealed by AFLP analysis", Plant Pathol, 60, pp.474~482 (2011)
- Chen H, "Molecular phylogeny of Colletotrichum (Sordariomycetes: Glomerellaceae) inferred from multiple gene sequences", Genet. Mol. Res, 14, pp.13649~13662 (2015)
- 쩐티펑지, "Streptomyces padanus for the Biological Control of Phytophthora capsici on Pepper Plants", 농업생명과학연구, 47(4), pp.1~9 (2013), 농업생명과학연구원
- 최용호, "고추 탄저병균에 대한 13종 살균제의 살균활성", 농약과학회지, 10(1), pp.36~42 (2006), 한국농약과학회
- Davidse LC., "Benzimidazole fungicides: mechanism of action and biological impact", Ann. Rev. Phytopathol, 24, pp.43~65 (1986)
- Dias JS., "Nutritional quality and health benefits of vegetables: a review", Food Nutr. Sci, 3, pp.1354~1374 (2012)
- Dice LR., "Measures of the amount of ecologic association between species", Ecology, 26, pp.297~302 (1945)
- Garcia-Vallvé S, "Horizontal gene transfer in glycosyl hydrolases inferred from codon usage in Escherichia coli and Bacillus subtilis", Mol. Biol. Evol, 16, pp.1125~1134 (1999)
- Guerber JC, "Characterization of diversity in Colletotrichum acutatum sensu lato by sequence analysis of two gene introns, mtDNA and intron RFLPs, and mating compatibility", Mycologia, 95, pp.872~895 (2003)
- 황수경, "Sensitivity of Colletotrichum spp. Isolated from Grapes in Korea to Carbendazim and the Mixture of Carbendazim Plus Diethofencarb", The Plant Pathology Journal, 26(1), pp.49~56 (2010), 한국식물병리학회
- 임진현, "고추 탄저병균의 배양형 변이 그리고 병원성 차이", 식물병 연구, 10(3), pp.203~208 (2004), 한국식물병리학회
- 장진성, "SSR 분자마커를 이용한 튀김옥수수 자식계통 집단의 구조 및 association 분석", 한국육종학회지, 45(4), pp.358~368 (2013), 한국육종학회[DOI]
- Kelemu S, "Genetic diversity in South American Colletotrichum gloeosporioides isolates from Stylosanthes guianensis, a tropical forage legume", Eur. J. Plant Pathol, 105, pp.261~272 (1999)
- 김대현, "고추 흰가루병균(Leveillula taurica)의 발아특성 및 접종법", 식물병 연구, 15(3), pp.187~192 (2009), 한국식물병리학회
- 김가혜, "청양 지역 고추와 구기자에서 분리한 탄저병균의 동정, 균학적 특징 및 살균제 저항성", 농약과학회지, 19(3), pp.335~344 (2015), 한국농약과학회[DOI]
- 김준태, "Characterization of Colletotrichum Isolates Causing Anthracnose of Pepper inKorea", The Plant Pathology Journal, 24(1), pp.17~23 (2008), 한국식물병리학회
- 김흥태, "살균제 chlorothalonil에 대한 고추 탄저병균의 감수성 변화", 농약과학회지, 8(3), pp.231~237 (2004), 한국농약과학회
- "Production of chili pepper and sesame" (2016)[URL]
- 이영기, "고추에서 분리된 Ralstonia solanacearum 계통의 생리, 생화학 및 유전적 특성", 식물병 연구, 19(4), pp.265~272 (2013), 한국식물병리학회[DOI]
- Mari M, "First report of Colletotrichum acutatum causing bitter rot on apple in Italy", Plant Dis, 96, pp.144~ (2012)
- Moral J, "Elucidation of the disease cycle of olive anthracnose caused by Colletotrichum acutatum", Phytopathology, 99, pp.548~556 (2008)
- Peres NA, "Life styles of Colletotrichum acutatum", Plant Dis, 89, pp.784~796 (2005)
- Prihastuti H, "Characterization of Colletotrichum species associated with coffee berries in northern Thailand", Plant Dis, 39, pp.89~109 (2009)
- 신종환, "Characterization of the Maize Stalk Rot Pathogens Fusarium subglutinans and F. temperatum and the Effect of Fungicides on Their Mycelial Growth and Colony Formation", The Plant Pathology Journal, 30(4), pp.397~406 (2014), 한국식물병리학회[DOI]
- Sicard D, "Genetic variability of Colletotrichum lindemuthianum in wild populations of common bean", Plant Pathol, 46, pp.355~365 (1997)
- Than PP, "Chili anthracnose disease caused by Colletotrichum species", J. Zhejiang Univ. Sci. B, 9, pp.764~778 (2008)
- Yang C, "Fungicide: Modes of action and possible impact on nontarget microorganisms", ISRN Ecol (2011)[DOI]