자두 탄저병균에 대한 살균제의 활성
In vitro Antifungal Activities of Fungicides against Japanese Plum Fruit Anthracnose Fungi
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BACKGROUND: In order to select a fungicide that can effectively control anthracnose disease in Japanese plum fruit, mycelial growth inhibition effect and spore germination inhibition effect of six fungicides were tested in vitro against six isolates of Colletotrichum acutatum and five isolates of C. gloeosporioides that were isolated from diseased Japanese plum fruit. METHODS AND RESULTS: Inhibitory effects of fungicides on mycelial growth were investigated after inoculating each isolate on potato dextrose agar amended with four discriminatory concentrations of each fungicide for 7 days at 25℃. For spore germination inhibitory effect, each isolate of the Colletotrichum spp. was cultured in potato dextrose agar for 7-14 days at 25°C. After adjusting the concentration of spores of each isolate to 1×106 mL-1 by diluting with 0.025% PDB, the spore suspension was mixed with each fungicide (1:4, v/v), and 60 μL aliquots were dispensed to sterile hole slide glass. Hole slide glasses were placed in a humidified box and incubated for 15 hours at 25°C. Then, spore germination was observed under an optical microscope. At recommended concentration of fungicide prochloraz manganese showed the highest mycelial growth inhibitory effect and dithianon showed the lowest mycelial growth inhibition. The EC50 values for the inhibition of spore germination by dithianon and pyraclostrobin were 0.069-0.126 μg/mL and 0.37-1.59 μ g/mL, respectively. Although benomyl, prochloraz manganese, azoxystrobin, and tebuconazole did not inhibit the spore germination, they appeared to restrain mycelial growth by abnormal growth of germ tube and mycelium after germination. CONCLUSION: Dithianon seemed to have preventive effect. Prochloraz manganese, azoxystrobin, and tebuconazole were likely to have control effect. Pyraclostrobin is considered to have both preventive and control effect against anthracnose disease of Japanese plum fruit.
Abstract
BACKGROUND: In order to select a fungicide that can effectively control anthracnose disease in Japanese plum fruit, mycelial growth inhibition effect and spore germination inhibition effect of six fungicides were tested in vitro against six isolates of Colletotrichum acutatum and five isolates of C. gloeosporioides that were isolated from diseased Japanese plum fruit. METHODS AND RESULTS: Inhibitory effects of fungicides on mycelial growth were investigated after inoculating each isolate on potato dextrose agar amended with four discriminatory concentrations of each fungicide for 7 days at 25℃. For spore germination inhibitory effect, each isolate of the Colletotrichum spp. was cultured in potato dextrose agar for 7-14 days at 25°C. After adjusting the concentration of spores of each isolate to 1×106 mL-1 by diluting with 0.025% PDB, the spore suspension was mixed with each fungicide (1:4, v/v), and 60 μL aliquots were dispensed to sterile hole slide glass. Hole slide glasses were placed in a humidified box and incubated for 15 hours at 25°C. Then, spore germination was observed under an optical microscope. At recommended concentration of fungicide prochloraz manganese showed the highest mycelial growth inhibitory effect and dithianon showed the lowest mycelial growth inhibition. The EC50 values for the inhibition of spore germination by dithianon and pyraclostrobin were 0.069-0.126 μg/mL and 0.37-1.59 μ g/mL, respectively. Although benomyl, prochloraz manganese, azoxystrobin, and tebuconazole did not inhibit the spore germination, they appeared to restrain mycelial growth by abnormal growth of germ tube and mycelium after germination. CONCLUSION: Dithianon seemed to have preventive effect. Prochloraz manganese, azoxystrobin, and tebuconazole were likely to have control effect. Pyraclostrobin is considered to have both preventive and control effect against anthracnose disease of Japanese plum fruit.
참고문헌 (21)
- Bernstein, B., "Characteristics of Colletotrichum from peach, apple, pecan, and other hosts", Plant Disease, 79(5), pp.478~482 (1995)[DOI]
- Biggs, A. R., "Relative susceptibility of selected apple cultivars to Colletotrichum acutatum", Plant Disease, 85(6), pp.657~660 (2001)[DOI]
- Cao, X., "Distribution and fungicide sensitivity of Colletotrichum species complexes from rubber tree in Hainan, China", Plant Disease, 101(10), pp.1774~1780 (2017)
- Choi, J. E., "Shot hole of peach and Japanese plum caused by Xanthomonas campestris pv. pruni and Erwinia nigrifluens in Korea", Research in Plant Disease, 6(1), pp.10~14 (2000)
- Freeman, S., "Management, survival strategies, and host range of Colletotrichum acutatum on Strawberry", HortScience, 43(1), pp.66~68 (2008)
- Freeman, S., "Characterization of Colletotrichum species responsible for anthracnose diseases of various fruits", Plant Disease, 82(6), pp.596~605 (1998)[DOI]
- 전창욱, "단감 탄저병 방제법 개선을 위한 살균제 선발 및 효과", 농약과학회지, 19(3), pp.312~316 (2015), 한국농약과학회[DOI]
- Jeon, J. Y., "Anthracnose of persimmon (Diospyros kaki) caused by Colletotrichum horii in Sangju, Korea", Plant Disease, 101(6), pp.1035~ (2017)
- Kenny, M. K., "Effect of fungicides in vitro and on detached berries on control of coffee berry anthracnose caused by Colletotrichum acutatum and C. gloeosporioides", Plant Protection Quarterly, 27(2), pp.59~63 (2012)
- 김완규, "Occurrence of Anthracnose on Peach Tree Caused by Colletotrichum Species", The Plant Pathology Journal, 24(1), pp.80~83 (2008), 한국식물병리학회
- Kurbetli, I., "First report of crown and root rot of plum caused by Phytophthora megasperma in Turkey", Plant Disease, 101(1), pp.260~ (2017)
- Dong Hyuk Lee, "Molecular and Cultural Characterization of Colletotrichum spp. Causing Bitter Rot of Apples in Korea", The Plant Pathology Journal, 23(2), pp.37~44 (2007), 한국식물병리학회
- 이용세, "자두 탄저병균의 분리 및 동정", 한국환경농학회지, 36(4), pp.299~305 (2017), 한국환경농학회[DOI]
- 임태헌, "2013년 경북 상주지역 감나무로부터 분리한 Colletotrichum gloeosporioides 탄저병균의 스테롤 생합성 저해 살균제에 대한 감수성", 농약과학회지, 19(3), pp.272~278 (2015), 한국농약과학회[DOI]
- 남명현, "딸기 탄저병 방제를 위한 정식 전 살균제 침지처리 효과", 식물병 연구, 20(1), pp.54~58 (2014), 한국식물병리학회[DOI]
- Peres, N. A. R., "Benomyl sensitivity of isolates of Colletotrichum acutatum and C. gloeosporioides from citrus", Plant Disease, 88(2), pp.125~130 (2004)[DOI]
- 류영현, "약제별 및 살포시기별 자두 검은점무늬병 방제효과", 식물병 연구, 18(4), pp.349~353 (2012), 한국식물병리학회[DOI]
- Sharma, M., "Colletotrichum gloeosporioides: An anthracnose causing pathogen of fruits and vegetables", Biosciences Biotechnology Research Asia, 12(2), pp.1233~1246 (2015)[DOI]
- Stevic, M., "Efficacy of fungicides with different modes of action in raspberry spur blight (Didymella applanata) control", Pesticide Phytomedicine (Belgrade), 32(1), pp.25~32 (2017)[DOI]
- Than, P. P., "Chilli anthracnose disease caused by Colletotrichum species", Journal of Zhejiang University Science B, 9(10), pp.764~778 (2008)[DOI]
- Yin, L. F., "Identification and characterization of three Monilinia species from plum in China", Plant Disease, 99(12), pp.1775~1783 (2015)[DOI]