자두 탄저병균의 분리 및 동정
Isolation and Characterization of Colletotrichum Isolates Causing Anthracnose of Japanese Plum Fruit
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참고문헌
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초록
탄저병이 발생한 자두에서 11개 탄저병균을 순수 분리하여 병원성을 검정한 후 PDA에 접종하여 25℃에서 7-10일동안 배양하면서 각 균주의 균사 생장속도, colony의 특징과색, 포자 형태 및 크기를 관찰하였다. 각 균주의 genomic DNA를 추출하여 rDNA-ITS 영역을 증폭한 다음, PCR을하여 염기서열을 해독하였다. 각 균주의 배양적 특성, 포자의 형태와 크기 및 염기서열을 NCBI GenBank의 염기서열과 상동성을 비교하여 동정한 결과 6개 균주는Colletotrichum acutatum으로, 5개 균주는 C. gloeosporioides로 동정되었다.
Abstract
BACKGROUND:Although the filamentous fungal pathogen Colletotrichum species causing anthracnose disease on various fruits including peach, apple, persimmon and grape, there is no report on Japanese plum in Korea. METHODS AND RESULTS: In 2016, diseased fruits showing typical anthracnose symptoms of Japanese plum were collected in market and ochards. Diseased tissue was cut off and disinfected subsequentlywith 70%ethanol for 1 min, and in 1% sodium hypochloride solution for 1 min, followed by three washes with sterile distilled water. The disinfected tissues were placed onto potato dextrose agar (PDA), and incubated at 25 ℃in the dark for 5 to 7 days. For single-spore isolation, conidia were scraped off the plate using a loop, and suspended with 10 mL sterile distilled water. One hundred microliter of the conidial suspension was spread on PDA plates and incubated at 25℃. Finally, one germinated conidiumwas transferred onto PDAplates. Morphological and cultural characteries of colonies and spores of isolated Colletotrichum were observed after 7 to 10 days incubation on PDA. Molecular identification of isolates were analyzed by comparing rDNA-ITS gene sequences with NCBI GeneBank. CONCLUSION: Of eleven isolates of Colletotrichum isolated from anthracnose diseased Japanese plum fruits, six were identified as C. acutatum, and five as C. gloeosporioides based on diagnostic characteristics such as colony growth rate, shape and size of conidia, and rDNAITS sequences. This is the first report of Colletotrichum causing the anthracnose on Japanese plum in Korea.
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