본문 바로가기
KERD
논문KCI 등재

배추 완전장 유전자 과발현 애기장대 형질전환체 활용 내염성 유전자 선발 및 유채 형질전환

Functional Screening of Salt Stress Tolerance Genes Using Transgenic Arabidopsis thaliana Lines Overexpressing Brassica rapa Full-length Genes and Brassica napus Transformation

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류농학

초록

Given that soil salinity significantly limits plant growth and production in agricultural land, research on salt stress is of particular agricultural relevance. In this study, for the purposes of functional screening of genes involved in salt stress responses, we selected approximately 651 transgenic Arabidopsis lines (157 independent full-length) from a transgenic Arabidopsis population overexpressing full-length Brassica rapa cDNAs. Initial screening indicated that the transgenic lines of 12 genes showed apparent salt tolerance phenotypes when exposed to NaCl at a concentration of 125 mM, among which, two genes (BrATL30 and BrZHD10) were selected for detailed characterization. The T3 progeny of these transgenic lines exhibited accelerated seed germination, often accompanied by faster root growth and higher survival rate, compared with wild-type plants under salt stress. Additionally, in order to examine the agricultural potential of the two selected B. rapa genes, we constructed BrATL30- and BrZHD10-overexpressing Brassica napus transgenic plants (BrATL30-OX and BrZHD10-OX), which showed apparent high salt stress-tolerant phenotypes compared with wild-type plants. Furthermore, we found that the basal expression of several saltand abiotic stress-responsive genes was higher in transgenic plants than in wild-type plants. Taken together, this study will provide two valuable functional genes related to salt stress tolerance.

Abstract

Given that soil salinity significantly limits plant growth and production in agricultural land, research on salt stress is of particular agricultural relevance. In this study, for the purposes of functional screening of genes involved in salt stress responses, we selected approximately 651 transgenic Arabidopsis lines (157 independent full-length) from a transgenic Arabidopsis population overexpressing full-length Brassica rapa cDNAs. Initial screening indicated that the transgenic lines of 12 genes showed apparent salt tolerance phenotypes when exposed to NaCl at a concentration of 125 mM, among which, two genes (BrATL30 and BrZHD10) were selected for detailed characterization. The T3 progeny of these transgenic lines exhibited accelerated seed germination, often accompanied by faster root growth and higher survival rate, compared with wild-type plants under salt stress. Additionally, in order to examine the agricultural potential of the two selected B. rapa genes, we constructed BrATL30- and BrZHD10-overexpressing Brassica napus transgenic plants (BrATL30-OX and BrZHD10-OX), which showed apparent high salt stress-tolerant phenotypes compared with wild-type plants. Furthermore, we found that the basal expression of several saltand abiotic stress-responsive genes was higher in transgenic plants than in wild-type plants. Taken together, this study will provide two valuable functional genes related to salt stress tolerance.

참고문헌 (52)

  1. Hugo Duarte Alves Horta, "Science and Technology in Portugal: From Late Awakening to the Challenge of Knowledge Integrated Communities. In Neave, G., and Amaral, A., (Eds.). Higher Education in Portugal 1974-2009: A Nation, A Generation", pp.1~33 (2012), Springer
  2. Bahmani K, "Molecular mechanisms of plant salinity tolerance : a review", Aust J Crop Sci, 9, pp.321~336 (2015)
  3. 백동원, "Salt cress 유전자의 형질전환을 통한 내염성 식물체 선별", Journal of Plant Biotechnology, 41(2), pp.81~88 (2014), 한국식물생명공학회
  4. Bartels D, "Drought and slat tolerance in plants", Crit Rev Plant Sci, 24, pp.1~36 (2005)
  5. Dassanayake M, "The genome of extremophile crucifer Thellungiella parvula", Nat Genet, 43, pp.913~918 (2013)
  6. Duan L, "Endodermal ABA signaling promotes lateral root quiescence during salt stress in Arabidopsis seedlings", Plant Cell, 25, pp.324~341 (2013)
  7. dos Reis SP, "Molecular cloning and characterization of a novel RING zinc-finger protein gene up-regulated under in vitro salt stress in cassava", Mol Biol Rep, 39, pp.1~7 (2012)
  8. Du QL, "GmRFP1encodes a previously unknown RING-type E3 ubiquitin ligase in Soybean(Glycine max)", Mol Biol Rep, 37, pp.685~693 (2010)
  9. Guzmán P, "The prolific ATL family of RING-H2ubiquitin ligases", Plant Signaling Behavior, 7, pp.1014~1021 (2012)
  10. He XJ, "AtNAC2, a transcription factor downstream of ethylene and auxin signaling pathways, is involved in salt stress response and lateral root development", Plant J, 44, pp.903~916 (2005)
  11. Hong JK, "Overexpression of Brassica rapa SHI-RELATEDSEQUENCE genes suppresses growth and development in Arabidopsis thaliana", Biotechnol Lett, 34, pp.561~156 (2012)
  12. 홍준기, "Identification and characterization of SHI family genes from Brassica rapa L. ssp. pekinensis", Genes & Genomics, 32(4), pp.309~317 (2010), 한국유전학회
  13. 홍준기, "Overexpression of a Brassica rapa MADS-box gene, BrAGL20, induces early flowering time phenotypes in Brassica napus", Plant Biotechnology Reports, 7(3), pp.231~237 (2013), 한국식물생명공학회
  14. 홍준기, "Overexpression of Brassica rapa GROWTH-REGULATING FACTOR genes in Arabidopsis thaliana increases organ growth by enhancing cell proliferation", Journal of Plant Biotechnology, 44(3), pp.271~286 (2017), 한국식물생명공학회
  15. 홍준기, "배추 GROWTH-REGULATING FACTOR 유전자 발현이 유채 기관크기에 미치는 영향", 한국육종학회지, 50(4), pp.378~386 (2018), 한국육종학회[DOI]
  16. Hu J, "Genomewide identification and expression pattern analysis of zinc-finger homeodomain transcription factors in tomato under abiotic stress", J Amer Soc Hort Sci, 143, pp.14~22 (2018)
  17. Ichikawa T, "The FOX hunting system : An alternative gain-of-function gene hunting technique", Plant J, 48, pp.974~985 (2006)
  18. Jiang Y, "Comparative proeomic analysis of NaCl stress-responsive proteins in Arabidopsis roots", J Exp Bot, 58, pp.3591~3607 (2007)
  19. Jiménez-López D, "Repertoire of plant RING E3 ubiquitin ligases revisited: New groups counting gene families and single genes", PLoS ONE, 13, pp.e0203442~ (2018)
  20. Jung HJ, "Genome-wide transcriptome analysis of two contrasting Brassica rapa doubled haploid lines under cold-stresses using Br135K oligomeric chip", PLoS ONE, 9, pp.e106069~ (2014)
  21. Khatun K, "Genome-wide analysis and expression profiling of zinc finger homeodomain(ZHD)family genes revels likely roles in organ development and stress responses in tomato", BMC Genomics, 18, pp.165~ (2017)
  22. Lata C, "Role of DREBs in regulation of abiotic stress responses in plants", J Exp Bot, 62, pp.4731~4748 (2011)
  23. 이강섭, "Ac", Ds 삽입 변이체를 이용한 벼 유전자 기능 연구, 2010, pp.37(2), 125~132[DOI]
  24. 이인호, "FOX hunting system을 이용한 배추 기능유전자 탐색", Journal of Plant Biotechnology, 37(2), pp.174~185 (2010), 한국식물생명공학회
  25. Leung J, "Arabidopsis ABA-response gene ABI1 : Features of a calcium-modulated protein phosphatase", Science, 264, pp.1448~1452 (1994)
  26. Liu J, "The Arabidopsis thaliana SOS2 gene encodes a protein kinase that is required for salt tolerance", Proc Natl Acad Sci USA, 97, pp.3730~3734 (2000)
  27. Munns R, "Mechanisms of salinity tolerance", Ann Review Plant Biology, 59, pp.651~681 (2008)
  28. Nakashima K, "Organization and expression of two Arabidopsis DREB2 genes encoding DRE-binding proteins involved in dehydration-and highsalinity-responsive gene expression", Plant Mol Biol, 42, pp.657~665 (2000)
  29. Parvaiz A, "Salt stress and phytobiochemical responses of plants-A review", Plant Soil Environ, 54, pp.88~99 (2008)
  30. Qiu QS, "Regulation of vacuolar Na+", H+ exchange in Arabidopsis thaliana by the salt-overly-sensitive(SOS)pathway, 2004, pp.279, 207~215
  31. Quintero FJ, "The SAL1 gene of Arabidopsis, encoding an enzyme with 3'(2'), 5'-bisphosphate nucleotide and inositol polyphosphate 1-phosphatase activities, increases salt tolerance in yeast", Plant Cell, 8, pp.529~537 (1996)
  32. Rus A, "Natural variants of AtHKT1enhance Na+ accumulation in two wild populations of Arabidopsis", PloS Gen, 2, pp.1964~1973 (2006)
  33. Rus A, "AtHKT1 is a salt tolerance determinant that controls Na entry into plant roots", Proc Natl Acad Sci USA, 98, pp.14150~14155 (2001)
  34. Sakuma Y, "Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expression", Proc Natl Acad Sci USA, 103, pp.18822~18827 (2006)
  35. Sakurai T, "RiceFOX : A database of Arabidopsis mutant lines overexpressing rice full-length cDNA that contains a wide range of trait information to facilitate analysis of gene function", Plant Cell Physiol, 52, pp.265~273 (2011)
  36. Seki M, "Functional genomics using RIKEN Arabidopsis thaliana full-length cDNAs", J Plant Res, 122, pp.355~366 (2009)
  37. Shalmani A, "Zinc finger-homeodomain genes : Evolution, functional differentiation, and expression profiling under flowering-related treatments and abiotic stresses in plants", Evol Bioinform, 15, pp.1~16 (2019)
  38. Shi H, "The putative plasma membrane Na+", H+antiporter SOS1 controls long-distance Na+ transporter in plants, 2002, pp.14, 465~477
  39. Song C, "Ectopic expression of an Arabidopsis dehydration-responsive element-binding factor DREB2C improves salt stress tolerance in crucifers", Plant Cell Rep, 33, pp.1239~1254 (2014)
  40. Song J, "An ATL78-like RING-H2 finger protein confers abiotic stress tolerance through interacting with RAV2 and CSN5B in tomato", Front Plant Sci, 7, pp.1305~ (2016)
  41. Tran LSP, "Co-expression of the stressinducible zinc finger homeodomain ZFHD1 and NAC transcription factors enhances expression of the ERD1 gene in Arabidopsis", Plant J, 49, pp.46~63 (2007)
  42. Vallejo AJ, "Germination variation in Arabidopsis thaliana accessions under moderate osmotic and salt stresses", Ann Bot, 106, pp.833~842 (2010)
  43. Wang H, "Genome-wide identification, evolution and expression analysis of the grape(Vitis vinifera L. )zinc finger-homeodomain gene family", Int J Mol Sci, 15, pp.5730~5748 (2014)
  44. Wang X, "The genome of the mesopolyploid crop species Brassica rapa", Nat Genet, 43, pp.1035~1039 (2011)
  45. Windhovel A, "Characterization of a novel class of plant homeodomain proteins that bind to the C4 phosphoenolpyruvate carboxylase gene of Flaveria trinervia", Plant Mol Biol, 45, pp.201~214 (2001)
  46. Xiong L, "Arabidopsis Book", pp.1~24 (2002), The American Society of Plant Biology
  47. Xiong L, "FIERY1 encoding an inositol polyphosphate 1-phosphatase is a negative regulator of abscisic acid and stress signaling in Arabidopsis", Genes Dev, 15, pp.1971~1984 (2001)
  48. Yang Q, "Overexpression of SOS(Salt Overly Sensitive)genes increases salt tolerance in transgenic Arabidopsis", Mol Plant, 2, pp.22~31 (2009)
  49. Yao K, "Cloning of dehydrin coding sequences from Brassica juncea and Brassica napus and their low temperature-inducible expression in germinating seeds", Plant Physiol Biochem, 43, pp.83~89 (2005)
  50. 윤대진, "식물의 고염 스트레스에 대한 반응 및 적응기작(총설)", Journal of Plant Biotechnology, 32(1), pp.1~14 (2005), 한국식물생명공학회
  51. Zhu J-K, "Genetic analysis of salt tolerance in Arabidopsis : Evidence for a critical role of Potassium nutrition", Plant Cell, 10, pp.1181~1191 (1998)
  52. Zhu J-K, "Plant salt tolerance", Trends Plant Sci, 6, pp.66~71 (2001)

관련 논문AI추천

이 간행물의 다른 논문 (25건)