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애기장대 H+-PPase(AVP1) 과발현 배추에서 바이오매스 증가와 내염성 향상

Increased biomass and enhanced tolerance to salt stress in Chinese cabbage overexpressing Arabidopsis H+-PPase (AVP1)

원희연|김창길|한증술|박미희
한국식물생명공학회 (Korean Society for Plant Biotechnology)|2012.12|Journal of Plant Biotechnology|제39권 제4호|pp. 253-260

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참고문헌

키워드

transformationChinese cabbagesalt toleranceAVP1photosystem II quantum yieldDAB staining
주제분류농학

초록

애기장대 액포 소재 H+-PPase(AVP1)의 과발현이 농업적으로 가치 있는 표현형을 나타낸다는 기 보고에 기초하여, AVP1 발현이 다른 종에서도 일관되게 바이오매스를증가시키고 염에 대한 내성을 향상시키는지를 확인하기위하여 본 연구에서는 AVP1 형질전환 배추 식물체를 획득한 후 고정계통을 육성하여 생리검정 재료로 사용하였다. 형질전환 배추 유식물체는 비형질전환 유식물체에비해 생장이 왕성하였으며 염스트레스에 대한 내성도 강하였다. 정상 재배조건에서 생장시킨 유식물체의 생체중과 건물중을 비교함으로써 형질전환에 의한 바이오매스증가 표현형을 확인하였으며 MS 염과 NaCl로 점차 염스트레스를 강화시키는 조건에서 광계II 양자수율을 추적,DAB 염색 실시 및 최종적으로 용토 탈염 후 회복 실험을수행함으로써 내염성 향상 표현형을 확인하였다.

Abstract

On the basis of the reported agriculturally valuable phenotypes resulted from ectopic overexpression of Arabidopsis vacuolar H+-PPase (AVP1), we generated the Chinese cabbage lines expressing AVP1 which then subjected to salt stress to determine the AVP1 expression if it consistently confers the capability for increasing biomass and enhancing tolerance to salinity in other species. Collectively, here we demonstrate that the transgenic young plants show more vigorous growth and higher tolerance to salt stress than wild-type ones. Increased biomass phenotype by AVP1expression was supported by comparing fresh and dry weights of transgenic and wild type plants grown under normal condition, while higher salt tolerance trait was confirmed by tracing the kinetics of photosystem II quantum yield and DAB-staining under gradually intensified salt stress induced by MS salt or NaCl, followed by normal condition.

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