배추(Brassica rapa)에서 분리한 스트레스 관련 후보 유전자 BrTSR53의 특성 분석
Molecular Characterization of a Putatively Stress-related Gene BrTSR53 Isolated from Chinese Cabbage (Brassica rapa)
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Crops are exposed to various environmental stresses. These have been affecting the growth of crops, resulting in thesevere loss of agronomic production in many countries. Therefore, development of new varieties of resistant crops is requiredto assure the desired productivity of crops in stress conditions. In this study, a putatively stress-related gene BrTSR53 was isolatedfrom Brassica rapa. The BrTSR53 is 481 bp long and contains ORF region of 234 bp. This ORF showed strong sequencesimilarities to the uncharacterized genes from Arabidopsis. The expression of BrTSR53 was determined by quantitative real-timePCR analysis. After 3 hr, the highest quantities of mRNA were revealed in cold and salt stress treatments. In drought stresstreatments, there was the highest expression after 36 hr. Therefore, it was confirmed that the ORF in BrTSR53 should be a genethat confer increased resistance to B. rapa growing in different stress conditions. The ORF region of BrTSR53 gene was clonedinto an expression vector, pYES-DEST52, and a new protein with molecular weight of 13 kDa was detected by western blotanalysis. Also, stress tolerance tests showed that BrTSR53-ORF transgenic yeast exhibited increased resistance to the salt stressescompared with the control. In conclusion, the present data predicts that novel ORF in BrTSR53 can serve as an important geneticresource for abiotic stress resistance.
Abstract
Crops are exposed to various environmental stresses. These have been affecting the growth of crops, resulting in thesevere loss of agronomic production in many countries. Therefore, development of new varieties of resistant crops is requiredto assure the desired productivity of crops in stress conditions. In this study, a putatively stress-related gene BrTSR53 was isolatedfrom Brassica rapa. The BrTSR53 is 481 bp long and contains ORF region of 234 bp. This ORF showed strong sequencesimilarities to the uncharacterized genes from Arabidopsis. The expression of BrTSR53 was determined by quantitative real-timePCR analysis. After 3 hr, the highest quantities of mRNA were revealed in cold and salt stress treatments. In drought stresstreatments, there was the highest expression after 36 hr. Therefore, it was confirmed that the ORF in BrTSR53 should be a genethat confer increased resistance to B. rapa growing in different stress conditions. The ORF region of BrTSR53 gene was clonedinto an expression vector, pYES-DEST52, and a new protein with molecular weight of 13 kDa was detected by western blotanalysis. Also, stress tolerance tests showed that BrTSR53-ORF transgenic yeast exhibited increased resistance to the salt stressescompared with the control. In conclusion, the present data predicts that novel ORF in BrTSR53 can serve as an important geneticresource for abiotic stress resistance.
참고문헌 (26)
- Bhatnagar-Mathur P, "Transgenic approaches for abiotic stress tolerance in plants : retrospect and prospects", Plant Cell Reports, 27, pp.411~424 (2008)
- Boyer JS, "Plant productivity and environment", Science, 218, pp.443~448 (1982)
- Chandna R, "Evaluation of candidate reference genes for gene expression normalization in Brassica juncea using real time quantitative RT-PCR", PLoS One, 7, pp.e36918~ (2012)
- Chen L, "A novel cold-regulated gene, COR25, of Brassica napus is involved in plant response and tolerance to cold stress", Plant Cell Rep, 30, pp.463~471 (2011)
- Cushman JC, "Genomic approaches to plant stress tolerance", Curr. Opin. Plant Biol, 3, pp.117~124 (2000)
- Dai X, "Overexpression of an R1R2R3 MYB gene, OsMYB3R-2, increases tolerance to freezing, drought, and salt stress in transgenic Arabidopsis", Plant Physiol, 143, pp.1739~1751 (2007)
- Diao G, "Functional characterization of a gluthathione S-transferase gene from Limonium bicolor in response to several abiotic stresses", Afr. J. Biotechnol, 9, pp.5060~5065 (2010)
- Hirashi H, "Enhancement of stress tolerance in Saccharomyces cerevisiae by overexpression of ubiquitin ligase Rsp5 and ubiquitin-conjugating enzymes", Biosci. Biotechnol. Biochem, 70, pp.2762~2765 (2006)
- Jellouli N, "Proteomic and transcriptomic analysis of grapevine PR10 expression during salt stress and functional characterization in yeast", Plant Mol. Biol. Rep, 28, pp.1~8 (2010)
- Jeong MJ, "Isolation and characterization of the gene encoding glyceraldehyde-3-phosphate dehydrogenase", Biochem. Biophys. Res. Commun, 278, pp.192~196 (2000)
- Jung YJ, "BrRZFP1 a Brassica rapa C3HC4-type RING zinc finger protein involved in cold, salt and dehydration stress", J. Plant Biol, 15, pp.274~283 (2013)
- 김효진, "콩의 Pathogenesis-Related 10 유전자를 이용한 내염성 벼 형질전환 계통 개발", 한국육종학회지, 42(5), pp.540~546 (2010), 한국육종학회
- Kushnirov VV, "Rapid and reliable protein extraction from yeast", Yeast, 16, pp.857~860 (2000)
- Lee J, "Molecular characterization of the Brassica rapa auxin-repressed, superfamily genes, BrARP1 and BrDRM1", Mol. Biol. Rep, 40, pp.197~209 (2013)
- 이상춘, "Transcriptome Analysis in Brassica rapa under the Abiotic Stresses Using Brassica 24K Oligo Microarray", Molecules and Cells, 26(6), pp.595~605 (2008), 한국분자세포생물학회
- Livak KJ, "Analysis of relative gene expression data using real-time quantitative PCR and the 2-△△C T method", Methods, 25, pp.402~408 (2001)
- Lyons JM, "Chilling injury in plants", Ann. Rev. Plant Physiol, 24, pp.445~466 (1973)
- Patterson BD, "Tomato pollen development: stages sensitive to chilling and a natural environment for the selection of resistant genotypes", Plant Cell Environ, 10, pp.363~368 (1987)
- Sataka T, "Sterility caused by cooling treatment at the flowering stage in rice plants. I. The stage and organ susceptible to cool temperature", Jpn. J. Crop Sci, 52, pp.207~214 (1983)
- Yean Joo Seo, "Overexpression of the Ethylene-Responsive Factor Gene BrERF4 from Brassica rapa Increases Tolerance to Salt and Drought in Arabidopsis Plants", Molecules and Cells, 30(3), pp.271~277 (2010), 한국분자세포생물학회
- Shinozaki K, "Gene networks involved in drought stress response and tolerance", J. Exp. Bot, 58, pp.221~227 (2007)
- Wang F, "A novel cold-inducible gene from Pak-choi (Brassica campestris ssp. chinensis), BcWRKY46, enhances the cold, salt and dehydration stress tolerance in transgenic tobacco", Mol. Biol. Rep, 39, pp.4553~4564 (2012)
- Xue T, "Cotton metallothionein GhMT3a, a reactive oxygen species scavenger, increased tolerance against abiotic stress in transgenic tobacco and yeast", J. Exp. Bot, 60, pp.339~349 (2009)
- Yadav NS, "A novel salt-inducible gene SbSI-1 from Salicornia brachiata confers salt and desiccation tolerance in E.coli", Mol. Biol. Rep, 39, pp.1943~1948 (2012)
- Yang R, "The reference genome of the halophytic plant Eutrema salsugineum", Front Plant Sci, 46, pp.1~14 (2013)
- 윤대진, "식물의 고염 스트레스에 대한 반응 및 적응기작(총설)", Journal of Plant Biotechnology, 32(1), pp.1~14 (2005), 한국식물생명공학회