싸리(Lespedeza bicolor) 추출물의 항산화성에 관한 연구
Antioxidant Activity and Inhibitive Effects on Human Leukemia Cells of Edible Mushrooms Extracts
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일상생활에서 식용 또는 약용으로 이용되고 있는 12종의 버섯 추출물을 제조하여 이들 추출물의 항산화능 검색과 함께 인간유래의 혈액암 세포주 및 정상 임파구세포의 성장에 미치는 영향에 대하여 조사하였다. 그 결과, 향버섯, 번데기동충하초, 만가닥버섯, 아가리쿠스, 영지버섯, 표고버섯 메탄올 추출물에서 30~60% 정도의 DPPH radical 소거 활성을 보였고, 목이버섯, 그물버섯, 새송이버섯에서 10~30%의 활성을 보였다. 또한 chinese hamster V79 cells에서 만가닥버섯, 번데기동충하초, 향버섯 메탄올추출물의 경우 H2O2로 유도된 세포 독성에 대한 39~53% 정도의 유의적인 생존율을 나타내었다. 그리고 인간유래 혈액암세포인 HL60과 U937에 대해 향버섯, 만가닥버섯, 번데기동충하초, 아가리쿠스, 영지버섯 추출물들이 높은 증식 억제 활성이 보였다. 특히 버섯 메탄올추출물 1.0 mg/mL 처리시 HL 60에 대해 향버섯이 70.5%로 가장 높은 저해율을, U937에 대해서는 번데기동충하초가 81.5%의 가장 높은 저해율을 나타내었다. 그리고 버섯 열수추출물들은 메탄올추출물보다 낮은 저해 활성을 보였다. 그러나 모든 버섯추출물들은 인간의 정상 임파구세포에 대해 95% 이상의 높은 생존율을 나타내어, 정상 세포에 대한 성장 저해 효과가 없음을 보여주었다. 번데기동충하초, 아가리쿠스, 만가닥버섯, 영지버섯, 향버섯 추출물은 항산화 할성 및 혈액암 세포주에 대한 증식 억제 활성이 높음을 확인할 수 있었다.
Abstract
The effect of 12 edible mushroom species on the antioxidant and cytotoxicity on cancer cells were studied. Methanol extracts of Lyophyllum ulmarium, Cordyceps militaris and Sarcodon aspratus showed 30~60% DPPH radical scavenging activity and 39~53% protective effects against the cytotoxicity of H2O2. Methanol extracts of Sarcodon aspratus, Lyophyllum ulmarium, Cordyceps militaris, Agaricus blazei and Ganoderma lucidum revealed high inhibitive activities in cytotoxicity on human leukemia cells such as promyelocytic leukemia cell (HL60) and histiocytic lymphoma cell (U937). Highest toxicity was observed against HL60 cells in Sarcodon aspratus methanol extracts showing 70.5% inhibition at 1mg/mL, whereas Cordyceps militaris methanol extracts showed 81.5% inhibition against U937 cells. Most water extracts of edible mushrooms exhibited the lowest effect against HL60 and U937 cells compared to methanol extracts. These extracts did not show cytotoxic effects against human lymphocyte. Results revealed 5 kinds of edible murshroom (Cordyceps militaris, Agaricus blazei, Lyophyllum ulmarium, Ganoderma lucidum and Sarcodon aspratus) have strong antioxidative and in vitro anticancer effects.
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