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겉보리에서 배양한 영지버섯 추출물의 항산화 및 항염증 효능 평가

Evaluation of Anti-Oxidant and Anti-Inflammatory Activities of Ganoderma lucidum Cultured on Hulled Barley

이영민|김연화|김진성|박동현|임병우|서경희|Mithun Ghosh|박강민
한국약용작물학회|2017.02|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제25권 제1호|pp. 29-36|DOI http://dx.doi.org/10.7783/KJMCS.2017.25.1.29

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키워드

Anti-OxidantLipopolysaccharideGanoderma lucidumAnti-InflammatoryHulled Barley

초록

Ganoderma lucidum cultured on hulled barley was investigated as a potential natural source of antioxidants and anti- inflammatory agents. Methods and Results: The yields from Ganoderma lucidum cultured on hulled barley water and ethanol extract were 17.69% and 25.77%, respectively. The antioxidant activity of Ganoderma lucidum cultured on hulled barley extracts was confirmed by various methods including assayss of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzo thiazoline-6-sulfonic acid) (ABTS), nitrite radical scavenging, and Fe3+ to Fe2+ reducing power activity. The ethanol extract ofGanoderma lucidum cultured on hulled barley showed improved DPPH, ABTS and nitrite radical scavenging activity compared with the water extract. After treat- ment of RAW264.7 cells with Ganoderma lucidum cultured on hulled barley ethanol extracts, the cell viability compared with the control was 92.82%,even at aconcentration of 3,000 ㎍/㎖.Theethanol extract inhibited reactiveoxygenspecies(ROS) generation inRAW264.7cellsstimulatedwithH2O2,even atlowconcentrations.Inaddition,theethanolextractshowedaninhibitoryeffectson the production of lipopolysaccharide-induced nitric oxide (NO) in RAW264.7 cells. Conclusions: This study suggests that the extract of Ganoderma lucidum cultured on hulled barley is a potential source of natural antioxidants and anti-inflammatory agents.

Abstract

Ganoderma lucidum cultured on hulled barley was investigated as a potential natural source of antioxidants and anti- inflammatory agents. Methods and Results: The yields from Ganoderma lucidum cultured on hulled barley water and ethanol extract were 17.69% and 25.77%, respectively. The antioxidant activity of Ganoderma lucidum cultured on hulled barley extracts was confirmed by various methods including assayss of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzo thiazoline-6-sulfonic acid) (ABTS), nitrite radical scavenging, and Fe3+ to Fe2+ reducing power activity. The ethanol extract ofGanoderma lucidum cultured on hulled barley showed improved DPPH, ABTS and nitrite radical scavenging activity compared with the water extract. After treat- ment of RAW264.7 cells with Ganoderma lucidum cultured on hulled barley ethanol extracts, the cell viability compared with the control was 92.82%,even at aconcentration of 3,000 ㎍/㎖.Theethanol extract inhibited reactiveoxygenspecies(ROS) generation inRAW264.7cellsstimulatedwithH2O2,even atlowconcentrations.Inaddition,theethanolextractshowedaninhibitoryeffectson the production of lipopolysaccharide-induced nitric oxide (NO) in RAW264.7 cells. Conclusions: This study suggests that the extract of Ganoderma lucidum cultured on hulled barley is a potential source of natural antioxidants and anti-inflammatory agents.

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