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화순 광산배수에 서식하는 토착 호산성 박테리아를 이용한 황철석의 용출 특성

Bioleaching of Pyrite from the Abandoned Hwasun Coal Mine Drainage using Indigenous Acidophilic Bacteria

홍영의|조강희|김계민|정경훈|박천영
한국자원공학회 (The Korean Society of Mineral and Energy Resources Engineers)|2009.10|한국자원공학회지 (Journal of the Korean Society of Mineral and Energy Resources Engineers)|제46권 제5호|pp. 521-535

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참고문헌

키워드

Extracellular polymeric substance (EPS)광산배수황철석Pyrite세포외중합체물질Indigenous acidophilic bacteria토착 호산성 박테리아Mine drainage water
주제분류자원공학

초록

화순 폐탄광 배수에 서식하는 토착 호산성 박테리아를 ATCC125 배양액에 접종하여 배양한 결과 막대모양의 호산성박테리아들이 배양되었다. SEM을 이용하여 토착박테리아의 특징과 부식되는 황철석 표면을 조사하였다. 배양액의 초기 pH는 3.89이었으나 토착 호산성 박테리아들이 성장함에 따라 점점 감소하여 배양 28일에는 2.3으로 나타났다. 배양 시간에 따른 토착호산성 박테리아의 개체수는 대략적으로 적응기, 성장기 및 사멸기로 나타났으며, ATCC125 배양액에서 토착 호산성 박테리아의 적응기는 약 15일 이상으로 나타났다. 화순 토착 호산성 박테리아를 ATCC125에서 배양하여 황철석 입자가 들어 있는 ATCC 배양액에 접종한 결과 101일 만에 수많은 박테리아 개체수가 황철석 표면에 부착하였다. 규칙적인 육각형의 공동이나 직선상의 파쇄대가 원래 황철석 표면에 형성되어 있었으며, 토착 호산성 박테리아들이 이들 장소를 선택적으로 공격하여 확장시키는 것으로 나타났다. 토착 호산성 박테리아가 황철석 표면을 산화시킬 때 박테리아로부터 생성되는 EPS물질이 황철석 표면을 피복하는 것으로 나타났으며 EPS 물질이 토착 박테리아가 황철석 표면에 부착하는 것을 도와주는 것으로 나타났다. 토착 호산성 박테리아를 황철석에 111일 동안 접종시킨 결과 대조 시료에 비해 박테리아 시료에서 Fe, Zn 및 Cu 함량이 각각 1.3배, 1.4배, 2배로 높게 용출되었다.

Abstract

The rod-shaped indigenous bacteria from the abandoned Hwasun coal mine drainage were cultivated in an ATCC125 medium. SEM was used to identify the morphology of the indigenous acidophilic bacteria and the corroded pyrite surface. On the indigenous bacteria growing in ATCC solution, the initial pH value (3.89) of ATCC solution was gradually decreased growth time and 28 days after in the pH value of ATCC solution was 2.3. The growth curve of indigenous bacteria in ATCC solution can be divided into lag, exponential and death period. The lag period in ATCC solution was up to 15 days. The indigenous bacteria inoculated in ATCC solution with supplement of pyrite grains, and then numerous indigenous bacteria were attached to the pyrite surface at 101 days after inoculation. The regular patterns of hexagonal cavities and the straight line of fractures were originally formed in the pyrite surface, and the indigenous acidophilic bacteria were selectively attacked and extensively widen these structures. When the indigenous bacteria oxidized the pyrite, the extracellular polymeric substance (EPS) was formed and covered with pyrite grains. The formation of EPS was probably secreted by the indigenous bacteria, and the EPS contributed to the indigenous bacteria’s ability to adhere to the pyrite surface. After 111 days inoculation with pyrite, the content of Fe, Zn and Cu enriched 1.3, 1.4 and 2 times more in the bacterial sample than the control sample.

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