Complement-mediated tail degradation of Neodiplostomum seoulense cercariae
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Abstract
The furcocercus cercariae of Neodiplostomum seoulense (Digenea: Neodiplostomidae) penetrate theskins of tadpoles and shed their tails. The speculated mechanism of this tail loss was physical efforts required to pro-duce a vigorous zigzag motion during skin penetration; no other mechanism has been proposed. We examined therelationship between the host serum and cercarial tail loss. Cercariae of N. seoulense were collected from experimen-tally infected Segmentina hemisphaerula, and lots of 300 cercariae were cultured in medium 199 contained severaltypes of sera. Cercarial tail degradation was induced in all media, but all the cercariae cultured except those culturedin media containing fetal bovine serum (FBS) died within 48 hr. After 72 hr cultivation in media containing FBS, cer-carial tail degradation was induced in 67.0%; in continuous cultivation 13.3% of larvae survived for 7 days. Tail degra-dation did not occur in the absence of serum and when serum was heat inactivated at 56°C for 30 min. The additionof 20 mM ethylenediaminetetraacetic acid (EDTA) blocked cercarial tail degradation completely. Moreover, the addi-tion of 20 mM MgCl2 restored tail degradation blocked by EDTA. These results suggest that the alternative comple-ment pathway is related with the N. seoulense cercarial tail degradation induced by serum.
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