Degranulation of human eosinophils induced by Paragonimus westermani-secreted protease
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Abstract
Eosinophil degranulation is considered to be a key effector function for the killing of helminthic worms and tissue inflammation at worm-infected lesion sites. However, relatively little data is available with regard to eosinophil response after stimulation with worm-secreted products which contain a large quantity of cysteine proteases. In this study, we attempted to determine whether the degranulation of human eosinophils could be induced by the direct stimulation of the excretory-secretory products (ESP) of Paragonimus westermani, which causes pulmonary paragonimiasis in human beings. Incubation of eosinophils for 3 h with Paragonimussecreted products resulted in marked degranulation, as evidenced by the release of eosinophil-derived neurotoxin (EDN) in the culture supernatants. Moreover, superoxide anion was produced by eosinophils in response to the ESP. The ESP-induced EDN release was found to be significantly inhibited when the ESP was pretreated with protease inhibitor cocktail, or with the cysteine protease inhibitor, E-64. These findings suggest that human eosinophils become degranulated in response to P. westermani-secreted proteases, which may contribute to in vivo tissue inflammation around the worms.
참고문헌 (20)
- "House dust mite allergens induce proinflammatory cytokines from respiratory epithelial cells", 169, pp.4572~4578 (2002)
- "Excystment of Paragonimus westermani metacercariae by endogenous cysteine protease", 81, pp.137~142 (1995)
- "Cysteine protease activities during maturation stages of Paragonimus westermani", 83, pp.902~907 (1997)
- "Activities of different cysteine proteases of Paragonimus westermani in cleaving human IgG", 35, pp.139~142 (1997)
- "novel mechanisms of signaling by serine proteases" (1998)
- "Cytokines directly induce degranulation and superoxide production from human eosinophils", 98, pp.371~381 (1996)
- "Kinetics of thrombin receptor cleavage on intact cells Relation to signaling", pp.9780~9786 (1993)
- "Protease-dependent activation of epithelial cells by fungal allergens leads to morphologic changes and cytokine production", 105, pp.1185~1193 (2000)
- "Dust mite proteolytic allergens induce cytokine release from cultured airway epithelium", 161, pp.3645~3651 (1998)
- "Biology of eosinophils", pp.242~260 (1998), Principles & Practice Mosby
- "Stimulation of degranulation from human eosinophils by platelet activating factor", 142, pp.3518~3526 (1989)
- "Arginine-specific protease from Porphyromonas gingivalis activates protease-activated receptors on human oral epithelial cells and induces interleukin-6 secretion", 69, pp.5121~5130 (2001)
- "Activation of protease-activated receptors by gingipains from Porphyromonas gingivalis leads to platelet aggregation a new trait in microbial pathogenecity", 97, pp.3790~3797 (2001)
- "Human eosinophils are activated by cysteine proteases and release inflammatory mediators", 111, pp.704~713 (2003)
- "Trypsin induces activation and inflammatory mediator release from human eosinophils through protease-activated receptor-2", j167, pp.6615~6622 (2001)
- "Ultrastructural evidence for eosinophil-parasite adherence reaction in human onchocercal lymphadenitis in the early period following diethylcarbamazine treatment", 33, pp.213~218 (1982)
- "Cysteine protease secreted by Paragonimus westermani attenuates effector functions of human eosinophils stimulated with immunoglobulin G", 69, pp.1599~1604 (2001)
- "Proteolytic activity of cysteine protease in excretory-secretory product of Paragonimus westermani newly excysted metacercariae pivotally regulates IL-8 production of human eosinophils", 22, pp.529~533 (2000)
- "Interaction of mite allergens Der p3 and Der p9 with protease-activated receptor-2 expressed by lung epithelial cells", 167, pp.1014~1021 (2001)
- "Mechanisms of human eosinophil activation by complement protein C5a and platelet-activating factor similar functional responses are accompanied by different morphologic alterations", 50, pp.34~50 47 (1995)