Antibody Responses in Sera of Different Mouse Strains Experimentally Infected with Neodiplostomum seoulense
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
To examine humoral immune responses in the host, we measured serum antibody levels in different strains of mice (ICR, BALB/c, and C3H) experimentally infected with Neodiplostomum seoulense. Specific IgG antibody levels were increased remarkably with little difference among 3 strains of mice infected with N. seoulense from day 7 to 35 post-infection. More target proteins of adult parasites reacted with IgG at the time when the worm recovery decreased compared with other times. More than 20 protein bands, from 14 kDa to 94 kDa in size, were separated from the crude antigen of N. seoulense adults by SDS-PAGE, and among them 26, 30, 35, 43, 54, 67, and 94 kDa proteins were the major antigenic proteins. The results suggest that significant IgG antibody responses occur against N. seoulense in mice and this may be related with expulsion of worms.
Abstract
To examine humoral immune responses in the host, we measured serum antibody levels in different strains of mice (ICR, BALB/c, and C3H) experimentally infected with Neodiplostomum seoulense. Specific IgG antibody levels were increased remarkably with little difference among 3 strains of mice infected with N. seoulense from day 7 to 35 post-infection. More target proteins of adult parasites reacted with IgG at the time when the worm recovery decreased compared with other times. More than 20 protein bands, from 14 kDa to 94 kDa in size, were separated from the crude antigen of N. seoulense adults by SDS-PAGE, and among them 26, 30, 35, 43, 54, 67, and 94 kDa proteins were the major antigenic proteins. The results suggest that significant IgG antibody responses occur against N. seoulense in mice and this may be related with expulsion of worms.
참고문헌 (23)
- Seo BS, "Fibricola seoulensis Seo, Rim and Lee, 1964 (trematoda) and fibricoliasis in man", Seoul Journal of Medicine, 31, pp.61~96 (1990)
- Hong ST, "Neodiplostomum seoulensis n. comb. (Trematoda: Neodiplostomidae)", JOURNAL OF PARASITOLOGY, 80, pp.660~663 (1994)
- Seo BS, "Studies on intestinal trematodes in Korea. V. A human case infected by Fibricola seoulensis (Trematoda: Diplostomatidae)", The Korean Journal of Parasitology, 20, pp.93~99 (1982), 대한기생충학회
- Hong ST, "Fifteen human cases of Fibricola seoulensis infection in Korea", The Korean Journal of Parasitology, 22, pp.61~65 (1984), 대한기생충학회
- Hong ST, "Ten human cases of Fibricola seoulensis infection and mixed one with Stellantchasmus and Metagonimus", The Korean Journal of Parasitology, 24, pp.95~97 (1986), 대한기생충학회
- Hong SJ, "Studies on intestinal trematodes in Korea: IX. Recovery rate and development of Fibricola seoulensis in experimental animals", The Korean Journal of Parasitology, 21, pp.224~233 (1983), 대한기생충학회
- Kook J, "Pathogenicity and lethality of a minute intestinal fluke, Neodiplostomum seoulense, to various strains of mice", JOURNAL OF PARASITOLOGY, 84, pp.1178~1183 (1998)
- Chai JY, "Food-borne intestinal trematode infections in the Republic of Korea", PARASITOLOGY INTERNATIONAL, 51, pp.129~154 (2002)
- Lee SH, "A histopathological study on the intestine of mice and rats experimentally infected by Fibricola seoulensis", The Korean Journal of Parasitology, 23, pp.58~72 (1985), 대한기생충학회
- Chai JY, "Intestinal mastocytosis and goblet cell hyperplasia in BALB", c and C3H mice infected with Neodiplostomum seoulense, 1998, pp.36, 109~119[DOI]
- Agger MK, "The antibody response in serum, intestinal wall and intestinal lumen of NMRI mice infected with Echinostoma caproni", JOURNAL OF HELMINTHOLOGY, 67, pp.169~178 (1993)
- Graczyk TK, "ELISA method for detecting anti-Echinostoma caproni (Trematoda: Echinostomatidae) immunoglobulins in experimentally infected ICR mice", JOURNAL OF PARASITOLOGY, 80, pp.544~549 (1994)
- Huh S, "IgA response in mice infected with Neodiplostomum seoulensis", The Korean Journal of Parasitology, 33, pp.55~60 (1995), 대한기생충학회
- Chai JY, "Genetic difference in susceptibility and fatality of three strains of mice experimentally infected with Neodiplostomum seoulense", JOURNAL OF PARASITOLOGY, 86, pp.1140~1144 (2000)
- Lowry OH, "Protein measurement with the folin phenol reagent", JOURNAL OF BIOLOGICAL CHEMISTRY, 193, pp.265~275 (1951)
- Brunet LR, "Immune responses during the acute stages of infection with the intestinal trematode Echinostoma caproni", Parasitology, 120, pp.565~571 (2000)
- Toledo R, "Kinetics of antibodies and antigens in serum of mice experimentally infected with Echinostoma caproni (Trematoda: Echinostomatidae)", JOURNAL OF PARASITOLOGY, 91, pp.978~980 (2005)
- Sotillo J, "Echinostoma caproni: Kinetics of IgM, IgA and IgG subclasses in the serum and intestine of experimentally infected rats and mice", EXPERIMENTAL PARASITOLOGY, 116, pp.390~398 (2007)
- Toledo R, "Echinostoma caproni: intestinal pathology in the golden hamster, a highly compatible host, and the Wistar rat, a less compatible host", EXPERIMENTAL PARASITOLOGY, 112, pp.164~1671 (2006)
- Ben-smithBen-Smith A, "The relationship between circulating and intestinal Heligmosomoides polygyrus-specific IgG1 and IgA and resistance to primary infection", PARASITE IMMUNOLOGY, 21, pp.383~395 (1999)
- Langford TD, "Central importance of immunoglobulin A in host defense against Giardia spp", INFECTION AND IMMUNITY, 70, pp.11~18 (2002)
- Simonsen PE, "Antibodies in the serum of golden hamsters experimentally infected with the intestinal trematode Echinostoma caproni", JOURNAL OF HELMINTHOLOGY, 65, pp.239~247 (1991)
- Choi MH, "A 54 kDa cysteine protease purified from the crude extract of Neodiplostomum seoulense adult worms", The Korean Journal of Parasitology, 37, pp.39~46 (1999), 대한기생충학회