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해수 및 시판 수산물에서 분리한 장염비브리오균(Vibrio parahaemolyticus) 의 항균제 내성 및 최소발육억제농도의 규명

Antimicrobial Resistance and Minimum Inhibitory Concentrations of Vibrio parahaemolyticus Strains Isolated from Seawater and Commercial Fisheries

김희대|박권삼|조의동
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)|2019.12|한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)|제52권 제6호|pp. 587-595|DOI https://doi.org/10.5657/KFAS.2019.0587

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참고문헌

키워드

Minimum inhibitory concentrationAntimicrobial resistanceVirulence genesVibrio parahaemolyticus
주제분류수산학

초록

Eighty-three Vibrio parahaemolyticus isolates from surface seawater in Gomso Bay on the west coast of Korea, and commercial fisheries from Gunsan fisheries center were analyzed for the presence of virulence genes and susceptibility to 30 different antimicrobials. All 83 isolates were examined for the presence of two virulence genes (tdh or trh) using polymerase chain reaction; however, neither gene was found in any of the isolates. A disk diffusion susceptibility test, showed that all of the strains studied were resistant to clindamycin, oxacillin, ticarcillin, and vancomycin, and also revealed varying levels of resistance to ampicillin (98.8%), penicillin G (95.2%), streptomycin (20.5%), cefoxitin (14.5%), amikacin (6.0%), cephalothin (4.8%), and erythromycin (3.6%). However, all of the strains were susceptible to 19 other antimicrobial agents, including cefepime, cefotaxime, chloramphenicol, gentamycin, nalidixic acid, sulfamethoxazole/trimethoprim, and trimethoprim. All 83 isolates (100%) were resistant to five or more classes of antimicrobials, and two strains exhibited resistance to ten antimicrobial agents. The average minimum inhibitory concentrations against V. parahaemolyticus of clindamycin, oxacillin, ticarcillin, and vancomycin were 55.9, 98.3, 499.3, and 44.3 μg/mL, respectively. These results provide new insight into the necessity for seawater sanitation in Gomso Bay and commercial fisheries, and provide evidence to help reduce the risk of contamination by antimicrobial- resistant bacteria.

Abstract

Eighty-three Vibrio parahaemolyticus isolates from surface seawater in Gomso Bay on the west coast of Korea, and commercial fisheries from Gunsan fisheries center were analyzed for the presence of virulence genes and susceptibility to 30 different antimicrobials. All 83 isolates were examined for the presence of two virulence genes (tdh or trh) using polymerase chain reaction; however, neither gene was found in any of the isolates. A disk diffusion susceptibility test, showed that all of the strains studied were resistant to clindamycin, oxacillin, ticarcillin, and vancomycin, and also revealed varying levels of resistance to ampicillin (98.8%), penicillin G (95.2%), streptomycin (20.5%), cefoxitin (14.5%), amikacin (6.0%), cephalothin (4.8%), and erythromycin (3.6%). However, all of the strains were susceptible to 19 other antimicrobial agents, including cefepime, cefotaxime, chloramphenicol, gentamycin, nalidixic acid, sulfamethoxazole/trimethoprim, and trimethoprim. All 83 isolates (100%) were resistant to five or more classes of antimicrobials, and two strains exhibited resistance to ten antimicrobial agents. The average minimum inhibitory concentrations against V. parahaemolyticus of clindamycin, oxacillin, ticarcillin, and vancomycin were 55.9, 98.3, 499.3, and 44.3 μg/mL, respectively. These results provide new insight into the necessity for seawater sanitation in Gomso Bay and commercial fisheries, and provide evidence to help reduce the risk of contamination by antimicrobial- resistant bacteria.

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곰소만 해역 해수에서 분리한 장염비브리오(Vibrio parahaemolyticus)의 항균제 내성 및 최소발육억제농도의 규명김태옥, 김희대, 박권삼, 엄인선한국수산과학회 · 한국수산과학회지2016 · 제49권 제5호완도해역 해수에서 분리한 장염비브리오(Vibrio parahaemolyticus)의 항균제 내성 및 병원성 유전자의 특징김태옥, 김회대, 박권삼, 엄인선, 조상만한국수산과학회 · 한국수산과학회지2014 · 제47권 제3호금강 하구 해역의 해수에서 분리한 장염비브리오(Vibrio parahaemolyticus) 균의 특성 및 항균제 내성김희대, 박권삼, 이신혜한국수산과학회 · 한국수산과학회지2022 · 제55권 제6호시판수산물에서 분리한 Vibrio parahaemolyticus의 병원성 인자와 항균제 내성 현황김은희, 이예지한국수산과학회 · 한국수산과학회지2019 · 제52권 제6호남해안 패류양식장에서 분리한 Vibrio parahaemolyticus의 병원인자분포 및 항균제내성권지영, 김지회, 김풍호, 박용수, 유홍식, 이태식, 이희정, 박큰바위한국수산과학회 · 한국수산과학회지2016 · 제49권 제4호양식 굴(Crassostrea gigas)에서 분리된 장염비브리오균의독소 유전자 보유 및 항균제 감수성김수경, 김정완, 박보미, 송기철, 안세라, 오은경, 유홍식한국수산과학회 · 한국수산과학회지2016 · 제49권 제2호

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