가미신선불로단이 알츠하이머병 진단지표인 아밀로이드 단백독성과 APPr에 미치는 영향
Effects of KSM on the Cytotoxicity of Amyloid β Protein and the APP's Molecular Weight
1
조회
0
다운로드
1
인용
0
참고문헌
초록
Abstract
In order to evaluate the neuroprotective effects of Kamisinsunbulo-dan(KSM), the cytotoxicity of amyloid β and the recovering effect of KSM were checked at first. Then the viability of C6 cells was tested in comparison with each concentration of KSM. The cytotoxicity of amyloid β(31-35) showed from 5 μM higher to 100 μM. And the recovering effect by KSM showed significantly at 100㎍/㎖ concentration. And the cell viability was shown significantly over 200 ㎍/㎖ of KSM. This is thought that the viability has some relation to length of culturing duration, 6 to 12 hrs. Lastly in the western blotting of APP, the amount of low molecule's APP was decreased. So the APP form ratio(APPr) changed to increase, and it meant that KSM can be used to lower the toxic APP, and can be a candidate for Alzheimer's disease.
참고문헌 (16)
- "Amyloid precursor protein(APP) and the biology of proteolytic processing relevance to Alzheimer's disease", 35(11), pp.1505~1535 (2003)
- "New frontiers in Alzheimer's disease genetics", 181(184) (2001)
- "The amyloid deposits in Alzheimer's disease", pp.2 357~369 (1984)
- "Alzheimer's disease: Genes, proteins, and therapy", 81, pp.741~766 (2001)
- "Early stages of probable Alzheimer disease are associated with changes in platelet amyloid precursor protein forms", 23, pp.207~210 (2002)
- "가미신선불로단이 ibotenic acid손상에 의해 유도된 치매 백서의 학습 및 기억장애에 미치는 영향", 16(6), pp.1152~ (2002)
- "The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor. Nature 325" (1987)
- "Genomic organization of the human amyloid beta-protein precursor gene. Gene 87" (1990)
- "Isolation and quantitation of soluble Alzheimer's-peptide from biological fluids. Nature 359" (1992)
- "Amyloid-β peptide is produced by cultured cells during normal metabolism. Nature359" (1992)
- "Oligomeric and fibrillar species of amyloid-β peptides differentially affect neuronal viability. The Journal of Biological Chemistry The Journal of Biological Chemistry", 277, pp.32046~32053 (2002)
- "Correlation between beta-amyloid peptide production and human APP-induced neuronal death.", 23, pp.1199~1204 (2002)
- "Early stages of probable Alzheimer disease are associated with changes in platelet amyloid precursor protein forms", 23(5), pp.207~210 (2002)
- "ApoE genotypeinfluences the biological effect of donepezil on APP metabolism in Alzheimer disease evidence from a peripheral model.", 12, pp.195~200 (2002)
- "The impact of risk factors of Alzheimer's disease in the Down syndrome.", 11, pp.267~270 (2003)
- "The impact of risk factors of Alzheimer's disease in the Down syndrome.", 11, pp.267~270 (2003)