Determination of Dimethyl Disulfide, Diallyl Disulfide, and Diallyl Trisulfide in Biopesticides Containing Allium Sativum Extract by Gas Chromatography
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초록
BACKGROUND: Garlic (Allium sativum) extract has beenallowed as commercial biopesticide material for pesticidalactivity in the Environmentally-friendly AgriculturePromotion Act. Nine commercial biopesticides containingA. sativum extract have been marketed in Korea. However,the analytical method of the active substances in thesematerials has not been studied. METHODS AND RESULTS: Cartridge clean-up methodfor the determination of dimethyl disulfide(DMDS), diallyldisulfide(DADS), and diallyl trisulfide(DATS) inbiopesticides containing A. sativum extract was developedand validated by gas chromatography(GC). The clean-upmethod was optimized using hydrophilic lipophilic balance(HLB) solid phase extraction(SPE) cartridges for thebioactive sulfides in biopesticides containing A. sativumextract, and the eluate was analyzed to quantify the DMDS,DADS, and DATS using the GC. The developed methodwas validated, and the LOQ and recovery rates of DMDS,DADS, and DATS were 0.226, 0.063, and 0.051 mg L-1 and80.6, 84.8, and 73.1%, respectively. From the ninecommercial biopesticide samples, contents of DMDS,DADS, and DATS were analyzed using the developedmethod and results showed <LOQ, <LOQ-113.4, and<LOQ-2.3 mg L-1, respectively. CONCLUSION: The developed method could be used indetermining the quality of biopesticides for themanufacture of commercial biopesticides containing A. sativum extract.
Abstract
BACKGROUND: Garlic (Allium sativum) extract has beenallowed as commercial biopesticide material for pesticidalactivity in the Environmentally-friendly AgriculturePromotion Act. Nine commercial biopesticides containingA. sativum extract have been marketed in Korea. However,the analytical method of the active substances in thesematerials has not been studied. METHODS AND RESULTS: Cartridge clean-up methodfor the determination of dimethyl disulfide(DMDS), diallyldisulfide(DADS), and diallyl trisulfide(DATS) inbiopesticides containing A. sativum extract was developedand validated by gas chromatography(GC). The clean-upmethod was optimized using hydrophilic lipophilic balance(HLB) solid phase extraction(SPE) cartridges for thebioactive sulfides in biopesticides containing A. sativumextract, and the eluate was analyzed to quantify the DMDS,DADS, and DATS using the GC. The developed methodwas validated, and the LOQ and recovery rates of DMDS,DADS, and DATS were 0.226, 0.063, and 0.051 mg L-1 and80.6, 84.8, and 73.1%, respectively. From the ninecommercial biopesticide samples, contents of DMDS,DADS, and DATS were analyzed using the developedmethod and results showed <LOQ, <LOQ-113.4, and<LOQ-2.3 mg L-1, respectively. CONCLUSION: The developed method could be used indetermining the quality of biopesticides for themanufacture of commercial biopesticides containing A. sativum extract.
참고문헌 (25)
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