UPLC를 활용한 데리스 추출물 함유 유기농자재 중 Rotenone과 Deguelin 정량분석
Quantitative Analysis of Rotenone and Deguelin in Biopesticides Containing Derris Extract by Ultra performance Liquid Chromatography
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초록
BACKGROUND: Three commercial biopesticides containingderris extract, which is permitted as a commercialbiopesticide substances by the Environmentally-friendlyAgriculture Promotion Act, have been marketed in Korea. But, the quantitative analytical method of active substancesfor crop protection in biopesticides containing derrisextract has not known. METHODS AND RESULTS: Solid phase extraction(SPE) cartridge clean-up method for the quantitativeanalysis of rotenone and deguelin in biopesticidescontaining derris extract was developed and validated byultra-performance liquid chromatography (UPLC). Theclean-up method was established using hydrophiliclipophilic balance (HLB) SPE cartridges for the bioactivesubstances in biopesticides containing derris extract, andthe eluate was analyzed to quantify the rotenone anddeguelin by the UPLC. LOQ and recovery rates of rotenoneand deguelin were 0.085 and 0.044 mg/L, 95.7 and 93.3%,respectively. The content of rotenone and deguelin in threebiopesticides containing derris extract were analyzed bythe developed method, the results showed 0.001-0.236 and<LOQ-0.258%, respectively. CONCLUSION: The developed method could beapplicable to determine the active substances and to controlthe quality of commercial biopesticide containing derrisextract.
Abstract
BACKGROUND: Three commercial biopesticides containingderris extract, which is permitted as a commercialbiopesticide substances by the Environmentally-friendlyAgriculture Promotion Act, have been marketed in Korea. But, the quantitative analytical method of active substancesfor crop protection in biopesticides containing derrisextract has not known. METHODS AND RESULTS: Solid phase extraction(SPE) cartridge clean-up method for the quantitativeanalysis of rotenone and deguelin in biopesticidescontaining derris extract was developed and validated byultra-performance liquid chromatography (UPLC). Theclean-up method was established using hydrophiliclipophilic balance (HLB) SPE cartridges for the bioactivesubstances in biopesticides containing derris extract, andthe eluate was analyzed to quantify the rotenone anddeguelin by the UPLC. LOQ and recovery rates of rotenoneand deguelin were 0.085 and 0.044 mg/L, 95.7 and 93.3%,respectively. The content of rotenone and deguelin in threebiopesticides containing derris extract were analyzed bythe developed method, the results showed 0.001-0.236 and<LOQ-0.258%, respectively. CONCLUSION: The developed method could beapplicable to determine the active substances and to controlthe quality of commercial biopesticide containing derrisextract.
참고문헌 (20)
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