유산균 발효한 현미상황버섯균사체 추출물의 β-Glucan 함량 및 주름개선 효과
β-Glucan Contents and Anti-wrinkling Effects of Brown Rice Phellinus linteus Mycelium Extracts Fermented with Lactobacillus plantarum
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목적: 본 연구는 주름개선 효과검정을 통해 현미상황버섯균사체의 주름개선 기능성 화장료 소재로서의 가능성을 확인하는데 그목 적이 있다. 방법: Lactobacillus plantarum 으로 현미상황버섯균사체를 배양하여 얻어진 추출물의 β-glucan 함량, β-glucan 분자량 측정, 세포독성평가, MMP-1 저해활성 측정, 콜라겐생합성 효과를 시험하였다. 결과: 현미상황버섯균사체의 β-glucan 함량 분석결과 미발효군, 발효군에서 각각 8.1, 12.8 g/100 g의 결과로 함량이 58% 증가하는 것을 입증하였으며, β-glucan의 분자량 측정을 실시한 결과, 2,500 m/z 이하의 저분자 β-glucan을 확인하여 발효공정이 고분자의 β-glucan을 저분자로 분해하는 것을 확인하였다. 추출물의 세포독성은 미발효군, 발효군 모두 2,000μ g/mL이하 농도에서 세포독성이 나타나지 않아 독성에 대한 안전성을 확인하였다. 자외선(UVB) 조사에 의한 세포생존률은 미발효군, 발효군 모두800 μg/mL 농도에서 가장 높은 세포생존율 효과를 나타냈다. Matrix metalloproteinase-1 (MMP-1)의 생성억제를 확인한 결과미발효군, 발효군 모두 400 μg/mL 이상의 농도에서 MMP-1의 생성억제의 효과가 나타내는 것을 확인하였으며, 발효군 1,000 μg/mL 이상의 농도에서는 adenosine 400 μg/mL 과 유사한 효과로 높은 저해활성을 나타내어 colalgenase 저해활성이 우수함을확인하였다. Pro-collagen type Ⅰ의 생합성량을 측정한 결과 대조군 대비 미발효군, 발효군 모두 3배 이상의 collagen 생합성 증가를 나타내었다. 결론: 위의 내용을 종합하여 주름개선용 기능성 화장료로서 현미상황버섯균사체 유산균 발효 배양물의 활용가능성을 제시하였다.
Abstract
Purpose: This study aimed to investigate the potential of brown rice Phellinus linteus as an anti-wrinkle material for cosmetics through examining anti-wrinkle effects. Methods: To check the anti-wrinkle effects of brown rice Phellinus linteus mycelium, we performed various experiments such as β-glucan content, β-glucan molecular weight, cell viability, matrix metalloproteinase-1 (MMP-1) inhibitory activity, and pro-collagen synthesis assay.Results: The β-glucan contents from brown rice Phellinus linteus mycelium extracts unfermented and fermented were 8.1, 12.8 g/100 g, rose up respectively and the fermented increased 58% compared with unfermented extract. The low molecular weights of the β-glucan such as less than 2500 m/z from the fermented was measured by MALDI-TOF mass spectrometry. We found that high molecular weight of β-glucan decomposed low molecular weight through fermentation. Measuring cytotoxicity of brown rice Phellinus linteus extracts in HS27 cells, cytotoxicity of both unfermented and fermented was not observed under 1,500 μg/mL concentration. After irradiating UVB treatment, HS27 cell viability of unfermented and fermented both showed the highest effect at 800 μg/mL concentration. The inhibitory activity of collagenase (MMP-1) of unfermented and fermented both showed the highest effect at a concentration of 400 μg/mL and the inhibitory activity of collagenase (MMP-1) of the fermented at a concentration of 1000 μg/mL had similar effects with adenosine at a concentration of 400 μg/mL. The amounts of biosynthesis of pro-collagen type I of the unfermented and fermented both increased more than three times compared with those of control. Conclusion: Through results, we identified anti-wrinkle effects of the brown rice Phellinus linteus and we anticipated the brown rice Phellinus linteus develop the functional cosmetic ingredient as anti-wrinkle effects
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