비마토프로스트가 섬유주단층세포층의 투과성에 미치는 영향
Effect of Bimatoprost on the Permeability of Trabecular Meshwork Cell Monolayer
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목적: Carboxyfluorescein을 이용하여 비마토프로스트가 섬유주단층세포층의 투과성에 미치는 영향을 알아보고자 하였다. 대상과 방법: Transwell의 내측 chamber에 인체의 섬유주세포를 단층으로 충만하게 배양한 후 투과성에 미치는 영향을 알아보기 위하여 벤잘코니움, 브리모니딘, 라타노프로스트, 비마토프로스트에 각각 1주일간 각각 노출시킨 다음 내측 chamber에 50 μMcarboxyfluorescein을 2시간 동안 노출시킨 후 외측 chamber에서 투과된 carboxyfluorescein의 농도를 측정하였다. 이때 세포의 생존은 MTT assay로 측정하였다. 결과: 각 약제는 1/1,000으로 희석한 농도에서 염류용액에 노출시킨 대조군에 비하여 세포의 생존에 유의한 영향을 미치지 않았다. 또한 브리모니딘, 라타노프로스트, 비마토프로스트는 1주일간 노출 후 대조군에 비하여 섬유주단층세포층의 투과도에 유의한 영향을미치지 않았으며(p＞0.05), 비마토프로스트와 라타노프로스트 사이에도 투과도의 차이가 나타나지 않았다(p＞0.05). 결론: 섬유주를 통한 방수유출을 촉진하는 것으로 알려진 비마토프로스트는 섬유주단층세포층에서 carboxyfluorescein의 투과도에영향을 미치지 않았다. 따라서 비마토프로스트가 섬유주세포를 통한 방수유출에 미치는 영향은 유의하지 않을 가능성이 있다.
Abstract
Purpose: To investigate the effects of bimatoprost on the permeability of cultured human trabecular meshwork cells (HTMC)monolayer. Methods: HTMCs were cultured until confluency in the inner Transwell chamber and then exposed to benzalkonium chloride, brimonidine,latanoprost or bimatoprost for 1 week. Carboxyfluorescein permeability through the HTMC monolayer was measuredusing a spectrofluorometer after 2 hours in the outer chamber. Cellular viability was assessed using the MTT assay. Results: Each drug diluted at 1/1000X did not affect the cellular survival (p > 0.05). Brimonidine, latanoprost and bimatoprost didnot affect the carboxyfluorescein permeability through the HTMC monolayer (p > 0.05). The carboxyfluorescein permeability wasnot different between latanoptost and bimatoprost after 1 week of exposure (p > 0.05). Conclusions: Bimatoprost, a drug known to increase trabecular outflow, does not affect the carboxyfluorescein permeabilitythrough the HTMC monolayer. Thus, the effect on the trabecular outflow of bimatoprost may not be significant.
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