테트라히드로졸린이 섬유주단층세포층의 투과도에 미치는 영향
Effect of Tetrahydrozoline on the Permeability of Trabecular Meshwork Cell Monolayer
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목적: 테트라히드로졸린(tetrahydrozoline, THZ)이 섬유주세포의 생존에 미치는 영향과 섬유주단층세포층의 투과도에 미치는 영향을 알아보고자 하였다. 대상과 방법: 일차배양한 인체의 섬유주세포에 아드레날린성 제제인 THZ를 0.01, 0.1, 1.0, 10.0 μM의 농도에 1일과 3일간 각각 노출시켰다. Transwell을 이용하여 단층으로 충만하게 배양한 섬유주세포에 THZ를 노출시켜 투과된 carboxyfluorescein의 농도를 측정하였다. 세포의 생존은 MTT assay로, 일산화질소의 생성은 Griess assay로 각각 측정하였다. 결과: THZ는 섬유주세포의 생존과 일산화질소의 생성에 유의한 영향을 미치지 않았다(p>0.05). THZ는 대조군에 비하여 노출 후 1일과 3일째에 각각 농도에 비례하여 섬유주단층세포층의 투과도를 유의하게 감소시켰다(p<0.05). 결론: THZ는 섬유주세포의 생존에는 영향을 미치지 않았으나 섬유주단층세포층의 투과도를 감소시켰다. 따라서 THZ는 섬유주세포층을 통한 방수유출을 감소시킬 가능성이 있을 것으로 생각된다.
Abstract
Purpose: To investigate the effects of tetrahydrozoline (THZ) on the survival of cultured human trabecular meshwork cells (HTMC) and the permeability of HTMC monolayer. Methods: Primary cultured HTMC were exposed to an adrenergic agonist (0.01, 0.1, 1.0 or 10 μM THZ) for 1 day and 3 days. Carboxyfluorescein permeability through the HTMC monolayer was measured using Transwell. Cellular viability and nitric oxide (NO) production were assessed using MTT and Griess assays, respectively. Results: THZ did not affect the cellular survival (p > 0.05) or NO production (p > 0.05). THZ significantly increased the carboxyfluorescein permeability through the HTMC monolayer in a dose-dependent manner compared with non-exposed control (p < 0.05) after exposure for 1 and 3 days. Conclusions: THZ does not affect the survival of HTMC but decreases the permeability of HTMC monolayer in a dose-dependent manner. Thus, THZ may possibly decrease trabecular outflow.
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