동공 산동제와 축동제가 섬유주세포에 미치는 영향
Effects of Pupil Dilation and Constriction Agents on Trabecular Meshwork Cells
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목적: 임상에서 사용되는 동공 산동제와 축동제가 배양된 섬유주세포의 생존과 일산화질소의 생성에 미치는 영향을 알아보고자 하였다. 대상과 방법: 섬유주세포를 일차배양한 후 트로피카미드, 싸이클로펜톨레이트, 아트로핀, 필로카핀에 0, 0.01, 0.1, 1.0 mg/ml의 농도로2시간 동안 각각 노출시킨 후 MTT assay와 Griess assay를 이용하여 세포의 생존율과 일산화질소의 생성량을 각각 측정하였다. 결과: 트로피카미드, 싸이클로펜톨레이트, 아트로핀, 필로카핀 모두 0.1 mg/ml의 농도에서부터 섬유주세포의 생존을 저하시켰다. 0.01mg/ml의 농도에서 트로피카미드, 싸이클로펜톨레이트, 아트로핀은 일산화질소의 생성을 증가시키고 필로카핀은 일산화질소의 생성을저하시켰으나 통계적으로 유의하지는 않았다. 결론: 임상적으로 사용되는 동공 산동제와 축동제는 고농도 또는 단기간에 자주 점안할 경우 사용할 경우 섬유주세포의 생존을 저하시킬 수 있으나 일산화질소의 생성에는 영향을 주지 않는 것으로 보아 단시간에 섬유주를 통한 방수유출에는 영향을 끼치지 않을 것으로생각한다.
Abstract
Purpose: To investigate the effects of pupil dilation and constriction agents on the survival and production of nitric oxide (NO) in cultured human trabecular meshwork cells (HTMC). Methods: Primarily cultured HTMC were exposed to 0, 0.01, and 0.1 mg/ml of tropicamide, cyclopentolate, atropine, or pilocarpine for 2 hours. Cellular survival and production of NO were assessed using the MTT assay and Griess assay,respectively. Results: Tropicamide, cyclopentolate, atropine, and pilocarpine decreased cellular survival at the concentration of 0.1 mg. At the concentration of 0.01 mg/ml, all agents decreased production of NO to some extent, although the reduction was not statistically significant. Conclusions: Pupil dilation and constriction agents may be toxic to HTMC if used at high concentrations or if used frequently in the short-term but may not affect trabecular outflow.
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