벤잘코니움, 미토마이신 C, 덱사메타존이 섬유주세포의 스트레스에 미치는 영향
Effect of Benzalkonium, Mitomycin-C and Dexamethasone on Stress in Trabecular Meshwork Cells
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목적: 벤잘코니움, 미토마이신 C, 덱사메타존이 섬유주세포의 스트레스에 미치는 영향을 알아보고자 하였다. 대상과 방법: Transwell의 상층 내부 chamber에 인체의 섬유주세포를 단층으로 충만하게 배양한 후 벤잘코니움과 미토마이신 C, 그리고 덱사메타존에 6시간 동안 각각 노출시킨 다음 상층 내부 chamber에 50 μm carboxyfluorescein을 2시간 동안 노출시킨 후 하층외부 chamber에 투과된 carboxyfluorescein의 농도를 532 nm에서 측정하였다. 이때 세포의 생존은 MTT assay로 측정하였다. 결과: 벤잘코니움, 미토마이신 C는 저농도에서는 세포의 생존에 영향을 미치지 않았으나 각각 농도에 비례하여 섬유주 단층세포층의투과성을 증가시켰으니 고농도에서는 세포의 생존을 감소시켰다. 0.1, 1.0 μm 덱사메타존은 세포의 생존이나 투과도에 영향을 미치지않았다. 결론: 벤잘코니움, 미토마이신 C는 섬유주세포의 생존에 영향을 미치지 않는 저농도에 노출된 경우에도 세포 독성을 나타내어 섬유주세포에서 스트레스를 유발할 수 있을 것으로 생각한다.
Abstract
Purpose: To investigate the effects of benzalkonium chloride (BAC), mitomycin C (MMC) and dexamethasone (DEX) on cellularstress in cultured human trabecular meshwork cell (HTMC) monolayers. Methods: HTMCs were cultured in the inner Transwell chamber until confluence and then were exposed to BAC, MMC or DEXfor 6 hours. The carboxyfluorescein permeability through the HTMC monolayer was measured using a spectrofluorometer at 532nm after 2 hours in the outer chamber. The 3-[4, 5 -dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay was usedto evaluate cellular viabilities. Results: The carboxyfluorescein permeability through the HTMC monolayer increased and cell survival decreased with 0.002%BAC (p < 0.05). Increased permeability without decreasing cell survival occurred with 0.05 μg/mL MMC. No effect on the permeabilityor cell survival was observed at 0.1 or 1.0 μm DEX (p > 0.05). Conclusions: BAC and MMC induced cellular toxicity and stress at lower concentrations but did not affect survival of culturedHTMCs.
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