본문 바로가기
KERD
논문KCI 등재

황화수소가 섬유주단층세포층 투과도 증가에 미치는 영향과 일산화질소의 상호작용에 관한 연구

Effects of Hydrogen Sulfide and Nitric Oxide on the Permeability of Cultured Trabecular Meshwork Cells

김재우|백민주|손명서
대한안과학회 (The Korean Ophthalmological Society)|2020.03|대한안과학회지 (Journal of The Korean Ophthalmological Society)|제61권 제3호|pp. 267-273

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

Nitric oxidePermeabilityHydrogen sulfideTrabecular meshwork
주제분류안과학

초록

목적: 황화수소(hydrogen sulfide, H2S)가 섬유주단층세포층의 투과성에 미치는 영향과 일산화질소와의 상호작용에 대해 알아보고자 하였다. 대상과 방법: 섬유주세포를 sodium hydrogen sulfide (NaHS) 0, 50, 100, 500 μM에 6시간 노출시킨 다음 transwell과 carboxyfluorescein을 이용하여 섬유주 단층세포층의 투과도를 측정하였다. Griess assay로 일산화질소(nitrogen oxide, NO)의 생성을 측정하였고, reverse transcription-polymerase chain reaction로 eNOS mRNA의 발현을 측정하였다. 저농도의 H2S에서 NO와 상승 작용이 일어나는지 알아보기 위하여 0, 1, 10 μM의 NaHS와 10 μM sodium nitropruside (SN)를 함께 투여하여 실험을 시행하였다. 결과: NaHS는 100 μM 이상에서 농도에 비례하여 섬유주단층세포층의 투과도를 증가시켰으며(p<0.05), NO의 생성과 eNOS mRNA의 발현에는 유의한 영향을 미치지 않았다(p>0.05). 0, 1, 10 μM의 NaHS와 10 μM SN을 함께 투여한 경우 섬유주단층세포층의 투과도와 NO의 생성, eNOS mRNA의 발현에는 각각 유의한 영향을 미치지 않았다(p>0.05). 결론: 고농도에서 H2S는 섬유주단층세포층의 투과도를 증가시켰으며 이러한 증가는 NO와는 무관하였다. 저농도에서 H2S는 NO와 상승 작용을 나타내지 않았다. 따라서 고농도의 H2S는 섬유주를 통한 방수 유출을 증가시킬 가능성이 있으며, 이러한 방수 유출의 증가는 NO와는 관련이 적을 것으로 생각된다.

Abstract

Purpose: To investigate the effects of hydrogen sulfide (H2S) on the permeability of a cultured human trabecular meshwork cells (HTMC) monolayer and its interaction with nitric oxide (NO). Methods: After exposing primary cultured HTMCs to 0, 50, 100, and 500 μM sodium hydrogen sulfide (NaHS) for 6 hours, the permeabilities through the HTMC monolayer were measured using a Transwell assay with carboxyfluorescein. The production of NO and eNOS mRNA expression were assessed using the Griess assay and reverse transcription-polymerase chain reaction, respectively. In addition, 0, 1, and 10 μM NaHS and 10 μM sodium nitroprusside (SN) were co-exposed to evaluate the possible synergistic effect of H2S and NO. Results: Greater than 100 μM NaHS increased the permeability through the HTMC monolayer in a dose-dependent manner (p < 0.05). These increased permeabilities were not accompanied by NO production or eNOS mRNA expression (p > 0.05). When 0, 1, and 10 μM NaHS and 10 μM SN were exposed together, there was no significant change of permeability, NO production, or eNOS mRNA expression (all, p > 0.05). Conclusions: NaHS at high concentrations increased the permeability of the HTMC monolayer, which was not affected by NO. NaHS at low concentrations did not show a synergistic effect with NO. Thus, H2S at high concentrations may increase trabecular outflow, which may not be associated with NO.

참고문헌 (49)

  1. Łowicka E, "Hydrogen sulfide (H2S) - the third gas of interest for pharmacologists", Pharmacol Rep, 59, pp.4~24 (2007)
  2. Chen CQ, "Hydrogen sulfide : third gaseous transmitter, but with great pharmacological potential", Acta Pharmacol Sin, 28, pp.1709~1716 (2007)
  3. Li L, "Hydrogen sulfide and cell signaling", Annu Rev Pharmacol Toxicol, 51, pp.169~187 (2011)
  4. Kabil O, "Enzymology of H2S biogenesis, decay and signaling", Antioxid Redox Signal, 20, pp.770~782 (2014)
  5. Kimura H, "Production and physiological effects of hydrogen sulfide", Antioxid Redox Signal, 20, pp.783~793 (2014)
  6. Kimura H, "The physiological role of hydrogen sulfide and beyond", Nitric Oxide, 41, pp.4~10 (2014)
  7. Elsey DJ, "Regulation of cardiovascular cell function by hydrogen sulfide(H2S)", Cell Biochem Funct, 28, pp.95~106 (2010)
  8. Kimura Y, "Hydrogen sulfide protects neurons from oxidative stress", FASEB J, 18, pp.1165~1167 (2004)
  9. Wallace JL, "Hydrogen sulfide-releasing anti-inflammatory drugs", Trends Pharmacol Sci, 28, pp.501~505 (2007)
  10. Zhang Y, "Hydrogen sulfide, the next potent preventive and therapeutic agent in aging and age-associated diseases", Mol Cell Biol, 33, pp.1104~1113 (2013)
  11. Kulkarni, M, "Endogenous production of hydrogen sulfide in isolated bovine eye", Neurochem Res, 36, pp.1540~1545 (2011)
  12. Goldblum D, "Prospects for relevant glaucoma models with retinal ganglion cell damage in the rodent eye", Vision Res, 42, pp.471~478 (2002)
  13. Lu M, "Hydrogen sulfide protects astrocytes against H2O2-induced neural injury via enhancing glutamate uptake", Free Radic Biol Med, 45, pp.1705~1713 (2008)
  14. Sakamoto K, "Hydrogen sulfide attenuates NMDA-induced neuronal injury via its anti-oxidative activity in the rat retina", Exp Eye Res, 120, pp.90~96 (2014)
  15. Liu H, "Hydrogen sulfide protects retinal ganglion cells against glaucomatous injury in vitro and in vivo", Invest Ophthalmol Vis Sci, 58, pp.5129~5141 (2017)
  16. Huang S, "Hydrogen sulfide supplement attenuates the apoptosis of retinal ganglion cells in experimental glaucoma", Exp Eye Res, 168, pp.33~48 (2018)
  17. Salvi A, "Effect of hydrogen sulfide donors on intraocular pressure in rabbits", J Ocul Pharmacol Ther, 32, pp.371~375 (2016)
  18. Robinson J, "Effects of hydrogen sulfide-releasing compounds on aqueous humor outflow facility in porcine ocular anterior segments, Ex Vivo", J Ocul Pharmacol Ther, 33, pp.91~97 (2017)
  19. Kida M, "Hydrogen sulfide increases nitric oxide production with calcium-dependent activation of endothelial nitric oxide synthase in endothelial cells", Eur J Pharm Sci, 48, pp.211~215 (2013)
  20. Hosoki R, "The possible role of hydrogen sulfide as an endogenous smooth muscle relaxant in synergy with nitric oxide", Biochem Biophys Res Commun, 237, pp.527~531 (1997)
  21. Bruce King S, "Potential biological chemistry of hydrogen sulfide(H2S)with the nitrogen oxides", Free Radic Biol Med, 55, pp.1~7 (2013)
  22. Nagpure BV, "Interaction of hydrogen sulfide with nitric oxide in the cardiovascular system", Oxid Med Cell Longev, 2016, pp.6904327~ (2016)
  23. Mosmann T, "Rapid colorimetric assay for cellular growth and survival : application to proliferation and cytotoxicity assays", J Immunol Methods, 65, pp.55~63 (1983)
  24. assay is a fast and reliable method for colorimetric determination of fungal cell densities, "Appl Environ Microbio", 1999, 3727~3729
  25. Green LC, "Analysis of nitrites and nitrates in biological fluids", Analytical Biochem, 126, pp.131~138 (1982)
  26. Grimes PA, "Carboxyfluorescein. A probe of the blood-ocular barriers with lower membrane permeability than fluorescein", Arch Ophthalmol, 100, pp.635~639 (1982)
  27. Araie M, "Carboxyfluorescein. A dye for evaluating the corneal endothelial barrier function in vivo", Exp Eye Res, 42, pp.141~150 (1986)
  28. Grimes PA, "Carboxyfluorescein transfer across the blood-retinal barrier evaluated by quantitative fluorescence microscopy : comparison with fluorescein", Exp Eye Res, 46, pp.769~783 (1988)
  29. Nakagawa S, "Toxicity evaluation of antiglaucoma drugs using stratified human cultivated corneal epithelial sheets", Invest Ophthalmol Vis Sci, 53, pp.5154~5160 (2012)
  30. Lei Y, "Oxidative stress impact on barrier function of porcine angular aqueous plexus cell monolayers", Invest Ophthalmol Vis Sci, 54, pp.4827~4835 (2013)
  31. Abe K, "The possible role of hydrogen sulfide as an endogenous neuromodulator", J Neurosci, 16, pp.1066~1071 (1996)
  32. Gadalla MM, "Hydrogen sulfide as a gasotransmitter", J Neurochem, 113, pp.14~26 (2010)
  33. Kimura H, "Hydrogen sulfide : from brain to gut", Antioxid Redox Signal, 12, pp.1111~1123 (2010)
  34. Persa C, "The distribution of cystathionine beta-synthase(CBS)in the eye : implication of the presence of a trans-sulfuration pathway for oxidative stress defense", Exp Eye Res, 83, pp.817~823 (2006)
  35. Wiederholt M, "Effect of diuretics, channel modulators, and signal interceptors on contractility of the trabecular meshwork", Ophthalmologica, 211, pp.153~161 (1997)
  36. Wiederholt M, "Current topics in membranes. The eye's aqueous Humor: from secretion to glaucoma", pp.163~202 (1998), Academic Press
  37. Wiederholt M, "Relaxation of trabecular meshwork and ciliary muscle by release of nitric oxide", Invest Ophthalmol Vis Sci, 35, pp.2515~2520 (1994)
  38. Behar-Cohen FF, "Decreased intraocular pressure induced by nitric oxide donors is correlated to nitrite production in the rabbit eye", Invest Ophthalmol Vis Sci, 37, pp.1711~1715 (1996)
  39. Dismuke WM, "NO-induced regulation of human trabecular meshwork cell volume and aqueous humor outflow facility involve the BKCa ion channel", Am J Physiol Cell Physiol, 294, pp.C1378~C1386 (2008)
  40. Zhao W, "The vasorelaxant effect of H2S as a novel endogenous gaseous KATP channel opener", EMBO, 20, pp.6008~6016 (2001)
  41. Zhao W, "Modulation of endogenous production of H2S in rat tissues", Can J Physiol Pharmacol, 81, pp.848~853 (2003)
  42. Kubo S, "Direct inhibition of endothelial nitric oxide synthase by hydrogen sulfide : contribution to dual modulation of vascular tension", Toxicology, 232, pp.138~146 (2007)
  43. Geng B, "Hydrogen sulfide downregulates the aortic L-arginine", nitric oxide pathway in rats, 2007, pp.293, R1608~R1618
  44. Yong QC, "Hydrogen sulfide interacts with nitric oxide in the heart : possible involvement of nitroxyl", Cardiovasc Res, 88, pp.482~491 (2010)
  45. Wu D, "An update on hydrogen sulfide and nitric oxide interactions in the cardiovascular system", Oxid Med Cell Longev, 2018, pp.4579140~ (2018)
  46. Roedl JB, "Homocystein levels in aqueous humor and plasma of patients with primary open-angle glaucoma", J Neural Transm(Vienna), 114, pp.445~450 (2007)
  47. Roedl JB, "Homocysteine in tear fluid of patients with pseudoexfoliation glaucoma", J Glaucoma, 16, pp.234~239 (2007)
  48. Patil A, "Sustained-release delivery system of a slow hydrogen sulfide donor, GYY 4137, for potential application in glaucoma", AAPS PharmSciTech, 18, pp.2291~2302 (2017)
  49. Perrino E, "New prostaglandin derivative for glaucoma treatment", Bioorg Med Chem Lett, 19, pp.1639~1642 (2009)

관련 논문AI추천

일산화질소가 섬유주단층세포층의 투과성에 미치는 영향김재우, 김현연대한안과학회 · 대한안과학회지2015 · 제56권 제5호섬유주세포에서 황화수소가 산화스트레스와 세포노화에 미치는 영향김윤영, 김재우, 박현진대한안과학회 · 대한안과학회지2020 · 제61권 제4호라타노프로스텐 부노드가 섬유주세포의 투과도에 미치는 영향김재우, 박형석대한안과학회 · 대한안과학회지2024 · 제65권 제2호테트라히드로졸린이 섬유주단층세포층의 투과도에 미치는 영향김재우, 황석진대한안과학회 · 대한안과학회지2017 · 제58권 제1호섬유주세포에서 발프로익산이 일산화질소의 생성과 일산화질소합성효소의 발현에 미치는 영향김재우, 박철호, 손명서대한안과학회 · 대한안과학회지2018 · 제59권 제6호섬유주세포에서 국소 탄산탈수효소억제제가일산화질소의 생성에 미치는 영향에 대한 연구김세은, 김재우, 홍정흠대한안과학회 · 대한안과학회지2014 · 제55권 제3호

이 간행물의 다른 논문 (14건)

본태눈꺼풀연축 환자에서 Letibotulinum Toxin A의 유효성과 안전성 평가안희배, 양재욱, 우경인, 나태윤, 지미정, 정도훈, 김남주, 손준혁, 김지현, 김성은, 정호경, 이정규, 유혜린, 장재우, 윤진숙, 이성복, 백지선, 양석우, 이상언pp. 227-234안저검사상 망막이 관찰되지 않는 진행 백내장의 시력예후와 술 후 만족도유종인, 강민석, 방슬기, 진경현pp. 235-242초광각안저촬영을 통한 시간경과에 따른 망막진탕의 변화에 대한 고찰백지원, 김은철, 홍승우, 나호, 강남여, 이안나pp. 243-249만성 중심장액맥락망막병증에서 경구 스피로놀락톤의 효과이태은, 정새롬, 조남천, 유인천, 정진구, 안민pp. 250-257거대세포바이러스 양성 및 음성 고안압포도막염환자군들의 임상적 특징 비교김진호, 이지영, 최진아pp. 258-266
원문 보기