Estradiol valerate와 letrozole에 의해 유발되는 다낭성난소증후군의 난소변화 비교
HISTOLOGIC COMPARISON OF POLYCYSTIC OVARY SYNDROME INDUCED BY ESTRADIOL VALERATE AND LETROZOLE
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목적다낭성난소증후군(polycystic ovary syndrome, PCOS)을 estradiol valerate (EV)와 letrozole (LE)로 유발할 수 있는데 인간의 다낭성난소증후군과 가장 유사한 질환동물 모델이 무엇인가를 알아보고자 하였다. 연구방법EV와 LE를 각각 랫드에 처리하여 PCOS를 유발시킨 후 희생시켜 난소를 적출하였다. 난소를 조직학적으로 관찰하여 다낭성 난소 변화 유발유무, 난소의 크기, 난포낭의 크기 및 수를 측정하고 난포 및 간질세포의 황체화 등을 비교 관찰하였다. 결과LE처리군은 EV처리군에 비하여 난소의 크기 및 난포낭의 수가 유의하게 증가하고 난포막세포의 황체화가 뚜렷하여 인간의 PCOS와 더유사한 소견을 보였다. 결론랫드에서 LE처리에 의한 PCOS는 PCOS질환유발 동물 모델로 더 적합한 것으로 평가된다.
Abstract
Objective To compare ovarian morphology of the estradiol valerate (EV)- and the letrozole (LE)-induced polycystic ovary syndrome (PCOS) in rat models. Methods EV were injected (IM) to 10 Sprague-Dawley rats and LE were administered (PO) to the other 10 Sprague-Dawley rats for 21days. Sixty days after single injection of EV and 21 days after LE administration, the animals were sacrifi ced and the ovaries were sectioned, measured and analyzed histopathologically. Results LE-treated group showed larger ovary bearing more abundant cystic follicles with luteinized theca cells. These fi ndings were more similar to human PCOS ovarian morphology. Conclusion LE-induced PCOS model is recommended to conduct PCOS-related animal experiments.
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