腎氣丸合蒼附導痰湯이 Estradiol Valerate로 유도된 흰쥐의 다낭성 난소에 미치는 영향
Effects of Shingihwanhapchangbudodam-Tang on the Estradiol Valerate-induced Polycystic Ovary in Rats
0
조회
0
다운로드
21
인용
0
참고문헌
키워드
초록
Purpose: Polycystic ovarian syndrome(PCOS) is a endocrine disorder and still remains as one of the common causes of anovulation in women of reproductive age. There are some evidences that nerve growth factor(NGF) is involved in the pathogenesis of PCOS. In this research, the effects of ShingihwanhapChangbudodam- Tang(SCT) on the estradiol valerate(EV)-induced polycystic ovary(PCO) were investigated in rats. Methods: PCO was induced by single intramuscular injection with EV(4㎎) in female rats. Rats in PCO control group(n=8) were EV injected and orally administrated distilled water for sixty days. Rats in SCT-treated group(n=8) were EV injected and orally administrated SCT for same duration. The weights of body, ovary and adrenal gland were measured. And also, ovarian histopathology and NGF immunohistochemistry were performed. Results: The weights of ovaries in SCT-treated group were significantly increased compared with control group. The numbers of secondly and mature follicles, and corpora lutea in SCT-treated group were significantly increased compared with control group. The numbers of atretic follicles were significantly decreased compared with control group. The expression of NGF positive reaction in the ovarian granulosa cells of SCT-treated group were lesser observed than control group. Conclusion: From the these results, we concluded that Shingihwanhap Changbudodam-Tang(SCT) has inhibitory effect on the development of EV-induced polycystic ovary. And it's effect may be related with decreased NGF activities in the ovary.
Abstract
Purpose: Polycystic ovarian syndrome(PCOS) is a endocrine disorder and still remains as one of the common causes of anovulation in women of reproductive age. There are some evidences that nerve growth factor(NGF) is involved in the pathogenesis of PCOS. In this research, the effects of ShingihwanhapChangbudodam- Tang(SCT) on the estradiol valerate(EV)-induced polycystic ovary(PCO) were investigated in rats. Methods: PCO was induced by single intramuscular injection with EV(4㎎) in female rats. Rats in PCO control group(n=8) were EV injected and orally administrated distilled water for sixty days. Rats in SCT-treated group(n=8) were EV injected and orally administrated SCT for same duration. The weights of body, ovary and adrenal gland were measured. And also, ovarian histopathology and NGF immunohistochemistry were performed. Results: The weights of ovaries in SCT-treated group were significantly increased compared with control group. The numbers of secondly and mature follicles, and corpora lutea in SCT-treated group were significantly increased compared with control group. The numbers of atretic follicles were significantly decreased compared with control group. The expression of NGF positive reaction in the ovarian granulosa cells of SCT-treated group were lesser observed than control group. Conclusion: From the these results, we concluded that Shingihwanhap Changbudodam-Tang(SCT) has inhibitory effect on the development of EV-induced polycystic ovary. And it's effect may be related with decreased NGF activities in the ovary.
참고문헌 (23)
- "임상부인과 내분비학 제2판", pp.181~187 (2001), 서울:고려의학
- "부인과학 제3판", pp.519~525 (1997), 서울: 칼빈서적
- "다낭성 난소 증후군의 최신지견", 31, pp.38~44 (2002)
- "다낭성 난소증후군에 관한 문헌적 고찰", 10(1), pp.73~91 (1997)
- "다낭성 난소증후군 환자의 한방 치료에 대한 임상적 고찰", 13(2), pp.437~451 (2000)
- "蔡小蓀治療婦科痼疾驗方拾萃", 17(1), pp.9~10 (1996)
- "健脾益腎化痰湯治療多囊卵巢綜合證30例", 7, pp.31~ (1999)
- "Effects of a single injection of estradiol valerate on the hypothalamic arcuate nucleus and on reproductive function in the female rat", endocri(logy), pp.501~512 (1978)
- "Development of the polycystic ovarian condition", pp.647~655 (1986)
- "다낭성난소증후군 치료의 최신지견", 48(8), pp.1851~1869 (2005)
- "Amenorrhoea associated with bilateral polycystic ovaries"
- "The role of hyperinsulinemia in the pathogenesis of ovarian hyperandrogenism"
- "여성생식의학", pp.219~226 (1998), 서울: 대관출판사
- "蒼附導痰湯이 Estradiol Valerate로 유발된 백서의 다낭성 난소에 미치는 影響", 15(2), pp.1~11 (2002)
- "三陰交子宮穴 전침 자극이 백서의 다낭성 난소에 미치는 영향", 16(2), pp.76~86 (2003)
- "Effects of electro-acupuncture on nerve growth factor and ovarian morphology in rats with experimentally induced polycystic ovaries", 63(5), pp.1497~1503 (2000)
- "實用婦科方劑學", pp.107~274 (1997), 北京: 人民衛生出版社
- "육미지황원과 신기환이 생쥐의 성숙도에 따른 배란과 수정란의 초기 발생에 미치는 영향", 13(1), pp.35~54 (2004)
- "Activation of ovarian sympathetic nerves in polycystic ovary syndrome", endocri(logy), pp.2690~2695 (1993)
- "Ovarian steroidal response to gonadotropins and beta-adrenergic stimulation is enhanced in polycystic ovarian syndrome role of the sympathetic innervation", endocri(logy), pp.2696~2703 (1993)
- "A role for trkA nerve growth factor receptors in mammalian ovulation", 137(1), pp.198~209 (1996)
- "Involvement of nerve growth factor in female sexual development", endocri(logy), pp.364~375 (1990)
- "Ovarian steroidal response to gonadotropins and beta-adrenergic stimulation is enhanced in polycystic ovarian syndrome role of the sympathetic innervation", endocri(logy), pp.2696~2703 (1993)