에스트로겐 수용체-알파 녹아웃 마우스에 발생한 난소 출혈다낭의 병리학적 특징과 출혈다낭에서의 에스트로겐 수용체-알파의 역할
Pathologic Characteristics of Ovarian Hemorrhagic Polycyst in Estrogen Receptor-alpha (ERα) Knockout Mice and Roles of ERα in Hemorrhagic Polycyst
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Background : Polycystic ovary syndrome (PCOS) is the most common endocrinopathy causing anovulation in women of childbearing age. It has been well established that estrogen receptor-alpha knockout (ERαKO) mice display several pathologic ovarian phenotypes of PCOS. The aims of this study were to determine ovarian pathology in new ERαKO mice using a CreloxP approach and intra-ovarian ERα function as regulating key aspects of PCOS. Methods : ERαKO mice, which were deficient in exon 3 of the ERα gene, were used. Immunohistochemical studies were done on ovaries of control and ERαKO mice using antibodies specific to ERα, ERβ, inhibin-α, and α-smooth muscle actin (SMA), as well as histochemical staining using Sudan black-B. Results : All ovaries of ERαKO mice were larger than control mouse ovaries and displayed a disrupted theca-interstitial tissue organization, multiple atretic follicles and multiple hemorrhagic cysts. None of the ERαKO mouse ovaries showed a corpus luteum. In addition, heavy deposition of Sudan black-B positive foamy cells was seen. The theca externa of preantral immature follicles and hemorrhagic cysts showed strong expression of α-SMA. Conclusions : ERαKO mice show hemorrhagic polycystic ovaries and hyperplasia of the theca externa. This study demonstrates that the ERa is the functional key to the pathogenesis of PCOS.
Abstract
Background : Polycystic ovary syndrome (PCOS) is the most common endocrinopathy causing anovulation in women of childbearing age. It has been well established that estrogen receptor-alpha knockout (ERαKO) mice display several pathologic ovarian phenotypes of PCOS. The aims of this study were to determine ovarian pathology in new ERαKO mice using a CreloxP approach and intra-ovarian ERα function as regulating key aspects of PCOS. Methods : ERαKO mice, which were deficient in exon 3 of the ERα gene, were used. Immunohistochemical studies were done on ovaries of control and ERαKO mice using antibodies specific to ERα, ERβ, inhibin-α, and α-smooth muscle actin (SMA), as well as histochemical staining using Sudan black-B. Results : All ovaries of ERαKO mice were larger than control mouse ovaries and displayed a disrupted theca-interstitial tissue organization, multiple atretic follicles and multiple hemorrhagic cysts. None of the ERαKO mouse ovaries showed a corpus luteum. In addition, heavy deposition of Sudan black-B positive foamy cells was seen. The theca externa of preantral immature follicles and hemorrhagic cysts showed strong expression of α-SMA. Conclusions : ERαKO mice show hemorrhagic polycystic ovaries and hyperplasia of the theca externa. This study demonstrates that the ERa is the functional key to the pathogenesis of PCOS.
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