The antioxidant and chemopreventive potentialities of Mosidae (Adenophora remotiflora) leaves
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Our study focused on the antioxidant activities of Mosidae leaf ethanol extract (MLE) and included measurements of reducing power, total phenolic compounds, DPPH radical scavenging activity, and hydroxyl radical scavenging activity. In order to determine whether or not MLE evidences any chemopreventive activities, experimental lung metastasis was induced via the i.v. inoculation of colon26-M3.1 carcinoma cells into BALB/c mice. Additionally, we attempted to characterize any possible cytotoxic effects in murine normal splenocytes and tumor cells (B16-BL6 and colon26-M3.1). The total phenolic content and reducing capacity were measured at 39 mg/100 mL and 1.24, respectively, whereas the DPPH and hydroxyl radical scavenging activities of MLE were measured to be 88.89% and 22.10%, respectively. Prophylactic i.v. treatment with MLE resulted in a dose-dependent and significant inhibition of lung metastasis. Specifically, a MLE dose of 200 ug per mouse resulted in an 88.90% inhibition of lung metastasis. For the cytotoxicity assay, MLE doses up to 100 ug/mL were not shown to affect the growth of normal murine splenocytes. Additionally, the survival of normal cells was not affected at MLE doses below 500 ug/mL. However, MLE doses up to 500 ug/mL reduced the percentage of tumor cell growth for B16BL6 (67% alive) and colon26-M3.1 (62% alive) cells.
Abstract
Our study focused on the antioxidant activities of Mosidae leaf ethanol extract (MLE) and included measurements of reducing power, total phenolic compounds, DPPH radical scavenging activity, and hydroxyl radical scavenging activity. In order to determine whether or not MLE evidences any chemopreventive activities, experimental lung metastasis was induced via the i.v. inoculation of colon26-M3.1 carcinoma cells into BALB/c mice. Additionally, we attempted to characterize any possible cytotoxic effects in murine normal splenocytes and tumor cells (B16-BL6 and colon26-M3.1). The total phenolic content and reducing capacity were measured at 39 mg/100 mL and 1.24, respectively, whereas the DPPH and hydroxyl radical scavenging activities of MLE were measured to be 88.89% and 22.10%, respectively. Prophylactic i.v. treatment with MLE resulted in a dose-dependent and significant inhibition of lung metastasis. Specifically, a MLE dose of 200 ug per mouse resulted in an 88.90% inhibition of lung metastasis. For the cytotoxicity assay, MLE doses up to 100 ug/mL were not shown to affect the growth of normal murine splenocytes. Additionally, the survival of normal cells was not affected at MLE doses below 500 ug/mL. However, MLE doses up to 500 ug/mL reduced the percentage of tumor cell growth for B16BL6 (67% alive) and colon26-M3.1 (62% alive) cells.
참고문헌 (29)
- Fiorentino A, "Isolation and structure elucidation of antioxidant polyphenols from quiunce (Cydonia vulgaris) peels", J Agric Food Chem, 56, pp.2660~2667 (2008)
- Valko M, "Free radicals, metals and antioxidants in oxidative stress-induced cancer", Chem Biol Interact, 160, pp.1~40 (2006)
- Valko M, "Free radicals and antioxidants in normal physiological functions and human disease", Int J Biochem Cell Biol, 39, pp.44~84 (2007)
- Colombo MP, "Interleukin-12 in anti-tumor immunity and immunotherapy", Cytokine Growth Factor Rev, 13, pp.155~168 (2002)
- Fisher M, "Anticancer effects and mechanisms of polysaccharide-K (PSK): Implications of cancer immuno-therapy", Anticancer Res, 22, pp.1737~1754 (2002)
- Saiki I, "Induction of tumoricidal macrophages and production of cytokines by synthetic muramyl dipeptide analogues", Vaccine, 6, pp.238~244 (1998)
- Yoon TJ, "Anti-metastatic activity of Acanthopanax senticosus extract and its possible immunological mechanism of action", J Ethnopharmacol, 93, pp.247~253 (2004)
- Efferth T, "Phytochemistry and pharmacogenomics of natural products derived from traditional Chinese medicine and Chinese materia medica with activity against tumor cells", Mol Cancer Ther, 7, pp.152~161 (2008)
- Schantz SP, "Evidence for the role of natural immunity in the control of metastatic spread of head and neck cancer", Cancer Immunol Immunother, 25, pp.141~148 (1987)
- Kim SH, "Flavor pattern and sensory characteristics of Adenophora remotiflora", Annals of Plant Resources Research, 260, pp.112~120 (2007), Duksung Women's University
- 김성향, "모시대(Adenophora remotiflora) 추출물의 휘발성 성분 및 항산화 활성", 한국식품과학회지, 39(2), pp.109~113 (2007), 한국식품과학회
- Kim SR, "Quality characteristics of Adenophora remotiflora for increasing the value as a food resource", Institute of Natural Science Research, 7, pp.117~125 (2001), Duksung Women's University
- AOAC, "Official methods of analysis of AOAC" (2005), Association of official analytical chemicals
- Oyaizu M, "Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine", Japanese Journal of Nutrition, 44, pp.307~315 (1986)
- Velioglu YS, "Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products", J Agric Food Chem, 46, pp.4113~4117 (1998)
- Huang D, "The chemistry beind antioxidant capacity assays", J Agric Food Chem, 53, pp.1841~1856 (2005)
- Chung SK, "Hydroxyl radical scavenging effects of species and scavengers from brown mustard (Brassica nigra)", Biosci Biotechnol Biochem, 61, pp.118~123 (1997)
- Yoon TJ, "Inhibitory effect Of Korean mistletoe (Viscum album coloratum) extract on tumour angiogenesis and metastasis of haematogenous and non-haematogenous tumour cells in mice", Cancer Lett, 97, pp.83~91 (1995)
- Lee SJ, "Antimetastatic and immunomodulating properties of a new herbal prescription, Bojung-bangam-tang", Int Immunopharmacol, 3, pp.147~157 (2003)
- Johnson TM, "Reactive oxygen species are downstream mediators of p53 dependent apoptosis", Proc Natl Acad Sci U S A, 통권 : 93, pp.11848~11852 (1996)
- Meir S, "Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves", J Agric Food Chem, 43, pp.1813~1817 (1995)
- Cho MJ, "A study on the antioxidant nutrients analysis and antioxidative activities of Adenophora remotiflora and Aster glehni", 석사, pp.2001
- Rice-Evan C, "Paganga. Antioxidant properties of phenolic compounds", Trends Plant Sci, 2, pp.152~159 (1997)
- Rininger JA, "Immunopharmacological activity of Echinacea preparations following simulated digestion on murine macrophages and human peripheral blood mononuclear cells", J Leukoc Biol, 68, pp.503~510 (2002)
- Schepetkin IA, "Macrophage immunomodulatory activity of polysaccharides isolated from Juniperus scopolorum", Int Immunopharmacol, 5, pp.1783~1799 (2005)
- Gillis CN, "Panax ginseng pharmacology: A nitric oxide link?", Biochem Pharmacol, 54, pp.1~20 (1997)
- O'Hara M, "A review of 12 commonly used medicinal herbs", Arch Fam Med, 7, pp.523~536 (1998)
- Timothy M, "Complementary and alternative medicine: herbs, phytochemicals and vitamins and their immunological effects", J Allergy Clin Immunol, 123, pp.283~294 (2009)
- Lee KH, "Antioxidant and anti-inflammatory activity of pine pollen extract in vitro", Phytother Res, 23, pp.41~48 (2009)