In Vitro Assay on Antioxidant Activity and Cytotoxicity of MethanolExtracts from Young Sprouts of Several Korean Salad Plants
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Antioxidant activity, total phenolics level and cytotoxicity of the methanol extracts from the young sprouts of 5 Korean woody salad plants were determined. Methanol extracts of Kalopanax pictus had the highest DPPH radical scavenging activity, with an IC50 value of 23.5mg 100g-1, and followed by Valeriana fauriei (43.1mg 100g-1), and Morus alba (>100mg 100g-1). Total phenolic content showed the highest amount in methanol extracts from Kalopanax pictus (23.7mg 100g-1), and followed by Valeriana fauriei (22.7mg 100g-1), Aralia elata (16.8mg 100g-1) and Morus alba (14.2mg 100g-1). In a MTT assay, methanol extracts of Aralia elata with IC50 values of 151.0 and 140.7ɠ mL-1 showed the most potent cytotoxicity on Calu-6 and MCF-7, respectively. On the other hand, methanol extracts of Kalopanax pictus (IC50 = 96.5ɠ mL-1) showed the highest activity against HCT-116, and followed by those of Aralia elata (123.3ɠ mL-1), and Actinidia arguta (162.0ɠ mL-1). Total phenolic content of the tested plant extracts was correlated with the DPPH radical scavenging activity, suggesting the phenolic compounds may contribute to the antioxidant properties of Korean salad plants.
Abstract
Antioxidant activity, total phenolics level and cytotoxicity of the methanol extracts from the young sprouts of 5 Korean woody salad plants were determined. Methanol extracts of Kalopanax pictus had the highest DPPH radical scavenging activity, with an IC50 value of 23.5mg 100g-1, and followed by Valeriana fauriei (43.1mg 100g-1), and Morus alba (>100mg 100g-1). Total phenolic content showed the highest amount in methanol extracts from Kalopanax pictus (23.7mg 100g-1), and followed by Valeriana fauriei (22.7mg 100g-1), Aralia elata (16.8mg 100g-1) and Morus alba (14.2mg 100g-1). In a MTT assay, methanol extracts of Aralia elata with IC50 values of 151.0 and 140.7ɠ mL-1 showed the most potent cytotoxicity on Calu-6 and MCF-7, respectively. On the other hand, methanol extracts of Kalopanax pictus (IC50 = 96.5ɠ mL-1) showed the highest activity against HCT-116, and followed by those of Aralia elata (123.3ɠ mL-1), and Actinidia arguta (162.0ɠ mL-1). Total phenolic content of the tested plant extracts was correlated with the DPPH radical scavenging activity, suggesting the phenolic compounds may contribute to the antioxidant properties of Korean salad plants.
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