The ingredients in Saengshik, a formulated health food, inhibited the activity of α-amylase and α-glucosidase as anti-diabetic function
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BACKGROUND/OBJECTIVES: We investigated total 26 ingredients of Saengshik which will be commercially produced as an anti-diabeticdietary supplement. SUBJECTS/METHODS: Thirteen vegetables, nine cereals, three legumes and one seed were extracted with aqueous ethanolfor 2 h at 60°C, and evaluated for their inhibitory effects against α-amylase and α-glucosidase and for total phenolic andflavonoid contents. RESULTS: All ingredients inhibited α-amylase activity except cabbage. Strong inhibitory activity of α-amylase was observed inleek, black rice, angelica and barley compared with acarbose as a positive control. Stronger inhibition of α-glucosidase activitywas found in small water dropwort, radish leaves, sorghum and cabbage than acarbose. All Saengshik ingredients suppressedα-glucosidase activity in the range of 0.3-60.5%. Most ingredients contained total phenols which were in the range of 1.2-229.4mg gallic acid equivalent/g dried extract. But, total phenolic contents were not observed in carrot, pumpkin and radish. Allingredients contained flavonoid in the range of 11.6-380.7 mg catechin equivalent/g dried extract. CONCLUSIONS: Our results demonstrate that Saengshik containing these ingredients would be an effective dietary supplementfor diabetes.
Abstract
BACKGROUND/OBJECTIVES: We investigated total 26 ingredients of Saengshik which will be commercially produced as an anti-diabeticdietary supplement. SUBJECTS/METHODS: Thirteen vegetables, nine cereals, three legumes and one seed were extracted with aqueous ethanolfor 2 h at 60°C, and evaluated for their inhibitory effects against α-amylase and α-glucosidase and for total phenolic andflavonoid contents. RESULTS: All ingredients inhibited α-amylase activity except cabbage. Strong inhibitory activity of α-amylase was observed inleek, black rice, angelica and barley compared with acarbose as a positive control. Stronger inhibition of α-glucosidase activitywas found in small water dropwort, radish leaves, sorghum and cabbage than acarbose. All Saengshik ingredients suppressedα-glucosidase activity in the range of 0.3-60.5%. Most ingredients contained total phenols which were in the range of 1.2-229.4mg gallic acid equivalent/g dried extract. But, total phenolic contents were not observed in carrot, pumpkin and radish. Allingredients contained flavonoid in the range of 11.6-380.7 mg catechin equivalent/g dried extract. CONCLUSIONS: Our results demonstrate that Saengshik containing these ingredients would be an effective dietary supplementfor diabetes.
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