면마과 3종 메탄올 추출물 및 분획물의 α-Glucosidase 억제 활성
α-Glucosidase Inhibition Activity of Methanol Extracts and Fractions Obtained from Three Dryopteridaceae Species
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초록
This study was performed to select adequate plant materials for developing a natural α-glucosidase inhibitor by analyzing α-glucosidase inhibition activity in fronds and rhizomes of three Dryopteridaceae species: Cyrtomium fortunei,Polystichum polyblepharum, and P. lepidocaulon. The highest α-glucosidase inhibitor obtained from frond of P. lepidocaulon (4.16㎍ • mL-1), and rhizome of C. fortunei (1.84㎍ • mL-1), showed much higher inhibition activity than acarbose (1413.70㎍ • mL-1). The biomass required to inhibit α-glucosidase by 50% was 0.04 ~ 0.35㎎ for frond and 0.03 ~ 0.10㎎for rhizome, and P. lepidocaulon required the least amount of fronds and P. lepidocaulon the least rhizomes. In frond, α-glucosidase inhibition activity was the highest in water fraction of C. fortunei (20.2㎍ • mL-1), and n-butanol fraction of P. lepidocaulon (9.33㎍ • mL-1) and P. polyblepharum (5.10㎍ • mL-1). In rhizome, it was the highest in n-butanol fractions of C. fortunei (19.76㎍ • mL-1) and P. polyblepharum (4.47㎍ • mL-1), and ethylacetate fraction of P. lepidocaulon (5.46㎍ • mL-1). The frond biomass required for 50% α-glucosidase inhibition was the lowest in the water fraction of C. fortunei (1.43㎎),and n-butanol fractions of P. lepidocaulon (1.10㎎) and P. polyblepharum (0.66㎎). The required biomass of rhizome was the lowest in the water fraction of C. fortunei (1.59㎎), and n-hexane fractions of P. lepidocaulon (0.04㎎) and P. polyblepharum (0.15㎎). The result of this study suggested that the three Dryopteridaceae species had high α-glucosidase inhibition activity with small biomass, which might have high value as materials for economical anti-diabetic medication.
Abstract
This study was performed to select adequate plant materials for developing a natural α-glucosidase inhibitor by analyzing α-glucosidase inhibition activity in fronds and rhizomes of three Dryopteridaceae species: Cyrtomium fortunei,Polystichum polyblepharum, and P. lepidocaulon. The highest α-glucosidase inhibitor obtained from frond of P. lepidocaulon (4.16㎍ • mL-1), and rhizome of C. fortunei (1.84㎍ • mL-1), showed much higher inhibition activity than acarbose (1413.70㎍ • mL-1). The biomass required to inhibit α-glucosidase by 50% was 0.04 ~ 0.35㎎ for frond and 0.03 ~ 0.10㎎for rhizome, and P. lepidocaulon required the least amount of fronds and P. lepidocaulon the least rhizomes. In frond, α-glucosidase inhibition activity was the highest in water fraction of C. fortunei (20.2㎍ • mL-1), and n-butanol fraction of P. lepidocaulon (9.33㎍ • mL-1) and P. polyblepharum (5.10㎍ • mL-1). In rhizome, it was the highest in n-butanol fractions of C. fortunei (19.76㎍ • mL-1) and P. polyblepharum (4.47㎍ • mL-1), and ethylacetate fraction of P. lepidocaulon (5.46㎍ • mL-1). The frond biomass required for 50% α-glucosidase inhibition was the lowest in the water fraction of C. fortunei (1.43㎎),and n-butanol fractions of P. lepidocaulon (1.10㎎) and P. polyblepharum (0.66㎎). The required biomass of rhizome was the lowest in the water fraction of C. fortunei (1.59㎎), and n-hexane fractions of P. lepidocaulon (0.04㎎) and P. polyblepharum (0.15㎎). The result of this study suggested that the three Dryopteridaceae species had high α-glucosidase inhibition activity with small biomass, which might have high value as materials for economical anti-diabetic medication.
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