인삼 캘러스의 Polyacetylene 생산에 미치는 여러 가지 화학물질의 효과
The Effects of Various Chemicals on the Production of Polyacetylene in Ginseng Callus in vitro Culture
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인삼 callus의 기내배양에 의해서 항암물질을 대량생산하기 위한 연구의 일환으로 인삼 callus의 polyacetylene생산에 미치는 전구물질 및 elicitor의 효과를 조사하였다. 5mg/l의 L-phenylalanine첨가배지에서 인삼 callus의 생장과 polyacethylene합성이 가장 양호하였으며 panaxynol뿐만 아니라 panaxydol도 형성되었다. 반면에 α-methyl-D.L.methionine과 D.L.- norleucine 첨가구에서는 polyacetylene이 전혀 생성되지 않았다. Elicitor로 사용되는 nigeran은 농도가 높아질수록 인삼 callus의 생장을 억제시키지만 polyacetylene생성에는 전혀 영향을 미치지 못했다. 그러나 chitosan은 0.5mg/l의 농도에서는 인삼 callus의 생장과 polyacetylene의 생성에 영향을 주지 못하였으나, 1mg/l 이상 처리구에서는 panaxynol은 검출되지 않았지만 panaxydol은 검출되었다.
Abstract
In order to develop the mass production method of anticancer compound-polyacetylenefrom tissue culture of Panax ginseng C.A. Mayer, these studies were carried out for theeffects of various chemicals used as precursor and elicitor in vitro. Ginseng callus culturedon the growth medium containing 5mg/l L-phenylalanine was well grown and detectedpolyacetylene compounds as well as panaxydol and panxynol. But same media containing α-methyl-D.L.methionine and D.L.-norleucine was was not detected any polyacetylene.Panaxydol, one of polyacetylene and active anticancer compound, was detected in callicultured on media with upper 1mg/l chitosan used as elicitor, but panaxynol was notdetected. Nigeran used as active elicitor, caused to decrease the growth of ginseng callus, anddont work as elictor on the biosynthesis of polyacetylene from ginseng callus.
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